Enhanced Chromogenic Sensitivity of Horseradish Peroxidase-Catalyzed Oxidative Reactions in the Presence of Betaine-Type Metabolite Analogs

2015 ◽  
Vol 88 (8) ◽  
pp. 1074-1082 ◽  
Author(s):  
Kotomi Takagi ◽  
Yasuhiro Kashima ◽  
Satoshi Fujii ◽  
Kazuya Koumoto
Keyword(s):  
Horseradish Peroxidase ◽  
Oxidative Reactions

scholarly journals QSPR Analysis of Peroxidase Substrates Reactivity

2009 ◽  
Vol 3 (4) ◽  
pp. 255-261
Author(s):  
Irina Romanovskaya ◽  
◽  
Victor Kuz’min ◽  
Olga Oseychuk ◽  
Eugeniy Muratov ◽  
...  
Keyword(s):  
Molecular Structure ◽  
Linear Regression ◽  
Horseradish Peroxidase ◽  
Quantitative Structure ◽  
Structure Property ◽  
Substituted Phenols ◽  
Phenol Derivatives ◽  
Oxidative Reactions ◽  
Structure Property Relationship ◽  
Electronic Parameters

Quantitative structure-property relationship (QSPR) analysis of phenol derivatives reactivity in the horseradish peroxidase catalyzed oxidative reactions was carried out. The statistic models, which describe the substituted phenols reactivity (Кm-1, Vmax) quite adequately, were obtained by multiple linear regression and partial least squares (PLS) methods. The electronic parameters of molecules, their lipophylicity, molecular refraction, and form parameters were used as descriptors for molecular structure. The obtained models allow to predict the reactivity of the new phenolic substrates with satisfactory reliability.

Effects of Vincristine on Endothelial Microtubules in the Spinal Cord

1976 ◽  
Vol 34 ◽  
pp. 58-59
Author(s):  
John L. Beggs ◽  
John D. Waggener ◽  
Wanda Miller
Keyword(s):  
Spinal Cord ◽  
Biological Activity ◽  
Horseradish Peroxidase ◽  
Transport Mechanism ◽  
Vasogenic Edema ◽  
Vesicular Transport ◽  
Close Proximity

Microtubules (MT) are versatile organelles participating in a wide variety of biological activity. MT involvement in the movement and transport of cytoplasmic components has been well documented. In the course of our study on trauma-induced vasogenic edema in the spinal cord we have concluded that endothelial vesicles contribute to the edema process. Using horseradish peroxidase as a vascular tracer, labeled endothelial vesicles were present in all situations expected if a vesicular transport mechanism was in operation. Frequently,labeled vesicles coalesced to form channels that appeared to traverse the endothelium. The presence of MT in close proximity to labeled vesicles sugg ested that MT may play a role in vesicular activity.

Comparative strengths and weaknesses of peroxidase and colloidal gold in pre- and post-embedding immunolabeling techniques

1987 ◽  
Vol 45 ◽  
pp. 1002-1005
Author(s):  
D. R. Abrahamson ◽  
P. L. St.John ◽  
E. W. Perry
Keyword(s):  
Electron Microscopy ◽  
Horseradish Peroxidase ◽  
Light Microscopy ◽  
Colloidal Gold ◽  
Light Microscope ◽  
Ultrastructural Localization ◽  
The Other ◽  
Quantitative Studies ◽  
Dense Suspension ◽  
Antigen Localization

Antibodies coupled to tracers for electron microscopy have been instrumental in the ultrastructural localization of antigens within cells and tissues. Among the most popular tracers are horseradish peroxidase (HRP), an enzyme that yields an osmiophilic reaction product, and colloidal gold, an electron dense suspension of particles. Some advantages of IgG-HRP conjugates are that they are readily synthesized, relatively small, and the immunolabeling obtained in a given experiment can be evaluated in the light microscope. In contrast, colloidal gold conjugates are available in different size ranges and multiple labeling as well as quantitative studies can therefore be undertaken through particle counting. On the other hand, gold conjugates are generally larger than those of HRP but usually can not be visualized with light microscopy. Concern has been raised, however, that HRP reaction product, which is exquisitely sensitive when generated properly, may in some cases distribute to sites distant from the original binding of the conjugate and therefore result in spurious antigen localization.

Unlabeled Antibody Immunocytochemistry Applied to Murine Mammary Tumor Cells

1975 ◽  
Vol 33 ◽  
pp. 390-391
Author(s):  
Wm. J. Arnold ◽  
J. Russo ◽  
H. D. Soule ◽  
M. A. Rich
Keyword(s):  
Horseradish Peroxidase ◽  
Mammary Tumor ◽  
Covalent Binding ◽  
Petri Dish ◽  
Gamma Chain ◽  
Mammary Tumor Virus ◽  
Mammary Tumor Cells ◽  
Murine Mammary Tumor ◽  
Tumor Virus ◽  
Unlabeled Antibody

Our studies of mammary tumor virus have included the application of the unlabeled antibody enzyme method of Sternberger to mammary tumor derived mouse cells in culture and observation with an electron microscope. The method avoids the extravagance of covalent binding of indicator molecules (horseradish peroxidase) with precious antibody locator molecules by relying instead upon specific antibody-antigen linkages. Our reagents included: Primary Antibody, rabbit anti-murine mammary tumor virus (MuMTV) which was antiserum 113 AV-2; Secondary Antibody, goat anti-rabbit IgG gamma chain (Cappel Laboratories); andthe Indicator, rabbit anti-horseradish peroxidase - horseradish peroxidase complex (PAP) (Cappel Labs.). Dilutions and washes were made in 0.05 M Tris 0.15 M saline buffered to pH 7.4. Cell monolayers, after light fixation in glutaraldehyde, were incubated in place by a protocol adapted from Sternberger and Graham and Karnovsky, then embedded by our usual method for monolayers. Reagents were confined to specific areas by neoprene 0-rings (Parker Seal Co.) reducing the amount of reagent needed to 50 microliters, 1/6th of that required to wet a 35 mm petri dish.

Oxidative Halogenation of Arenes, Olefins and Alkynes Mediated by Iodine(III) Reagents

2020 ◽  
Vol 17 ◽  
Author(s):  
Luis A. Segura-Quezada ◽  
Karina R. Torres-Carbajal ◽  
Yuvraj Satkar ◽  
Kevin A. Juárez Ornelas ◽  
Narendra Mali ◽  
...  
Keyword(s):  
Functional Groups ◽  
Oxidative Reactions ◽  
Recent Advances ◽  
Oxidative Halogenation ◽  
Synthetic Transformations

: Iodine(III)-based reagents has been broadly used in oxidative reactions for the structural functionalization with several functional groups. Among the more relevant and useful synthetic transformations using these hypervalent γ 3 -reagents, it can be found the fluorination, chlorination, bromination as well as the iodination protocols. Herein, we present some of the most representatives oxidative halogenation procedures of arenes, olefins and alkynes dating from the oldest to the more recent advances in the area, highlighting the discovery and application of new iodine(III)-based halogenating species.

Enhancement of the horseradish peroxidase-catalyzed chemiluminescent oxidation of cyclic diacyl hydrazides by 6-hydroxybenzothiazoles

1985 ◽  
Vol 145 (1) ◽  
pp. 96-100 ◽  
Author(s):  
Gary H.G. Thorpe ◽  
Larry J. Kricka ◽  
Eileen Gillespie ◽  
Susan Moseley ◽  
Robert Amess ◽  
...  
Keyword(s):  
Horseradish Peroxidase
Sign in / Sign up

Export Citation Format

Share Document