GenEPi: Piezo1-based fluorescent reporter for visualizing mechanical stimuli with high spatiotemporal resolution

2019 ◽  
Author(s):  
Ramona Jühlen
Keyword(s):  
Mechanical Stimuli ◽  
Spatiotemporal Resolution ◽  
Fluorescent Reporter ◽  
High Spatiotemporal Resolution

scholarly journals GenEPi: Piezo1-based fluorescent reporter for visualizing mechanical stimuli with high spatiotemporal resolution

2019 ◽  
Author(s):  
Sine Yaganoglu ◽  
Nordine Helassa ◽  
Benjamin M. Gaub ◽  
Maaike Welling ◽  
Jian Shi ◽  
...  
Keyword(s):  
Ion Channel ◽  
High Specificity ◽  
Mechanical Stimuli ◽  
Spatiotemporal Resolution ◽  
Fluorescent Reporter ◽  
Non Invasive ◽  
Live Tissue ◽  
Biological Responses ◽  
Mechanosensitive Ion Channel ◽  
Cellular Mechanosensing

AbstractMechanosensing is a ubiquitous process to translate external mechanical stimuli into biological responses during development, homeostasis, and disease. However, non-invasive investigation of cellular mechanosensing in complex and intact live tissue remains challenging. Here, we developed GenEPi, a genetically-encoded fluorescent intensiometric reporter for mechanical stimuli based on Piezo1, an essential mechanosensitive ion channel found in vertebrates. We show that GenEPi has high specificity and spatiotemporal resolution for Piezo1-dependent mechanical stimuli, exemplified by resolving repetitive mechanical stimuli of spontaneously contracting cardiomyocytes within microtissues, in a non-invasive manner.

Imaging collagen type I fibrillogenesis with high spatiotemporal resolution

2015 ◽  
Vol 149 ◽  
pp. 86-94 ◽  
Author(s):  
Dimitar R Stamov ◽  
Erik Stock ◽  
Clemens M Franz ◽  
Torsten Jähnke ◽  
Heiko Haschke
Keyword(s):  
Collagen Type ◽  
Collagen Type I ◽  
Type I ◽  
Spatiotemporal Resolution ◽  
High Spatiotemporal Resolution

scholarly journals Assessing PM2.5Exposures with High Spatiotemporal Resolution across the Continental United States

2016 ◽  
Vol 50 (9) ◽  
pp. 4712-4721 ◽  
Author(s):  
Qian Di ◽  
Itai Kloog ◽  
Petros Koutrakis ◽  
Alexei Lyapustin ◽  
Yujie Wang ◽  
...  
Keyword(s):  
United States ◽  
Spatiotemporal Resolution ◽  
High Spatiotemporal Resolution

Deep-learning on-chip light-sheet microscopy enabling video-rate volumetric imaging of dynamic biological specimens

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Xiaopeng Chen ◽  
Junyu Ping ◽  
Yixuan Sun ◽  
Chengqiang Yi ◽  
Sijian Liu ◽  
...  
Keyword(s):  
Deep Learning ◽  
Light Scattering ◽  
Light Sheet ◽  
High Contrast ◽  
Spatiotemporal Resolution ◽  
Volumetric Imaging ◽  
Light Sheet Microscopy ◽  
High Spatiotemporal Resolution ◽  
On Chip

Volumetric imaging of dynamic signals in a large, moving, and light-scattering specimen is extremely challenging, owing to the requirement on high spatiotemporal resolution and difficulty in obtaining high-contrast signals. Here...

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