On-demand, cell-free biomanufacturing of conjugate vaccines at the point-of-care

2019 ◽  
Author(s):  
Zhang-He Goh
Keyword(s):  
Point Of Care ◽  
Conjugate Vaccines ◽  
On Demand

Printed Iontophoretic‐Integrated Wearable Microfluidic Sweat‐Sensing Patch for On‐Demand Point‐Of‐Care Sweat Analysis

2021 ◽  
Vol 6 (4) ◽  
pp. 2000910
Author(s):  
Brince Paul ◽  
Silvia Demuru ◽  
Céline Lafaye ◽  
Mathieu Saubade ◽  
Danick Briand
Keyword(s):  
Point Of Care ◽  
Demand Point ◽  
On Demand

scholarly journals On-demand, cell-free biomanufacturing of conjugate vaccines at the point-of-care

2019 ◽  
Author(s):  
Jessica C. Stark ◽  
Thapakorn Jaroentomeechai ◽  
Tyler D. Moeller ◽  
Rachel S. Dubner ◽  
Karen J. Hsu ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Point Of Care ◽  
Cold Chain ◽  
Conjugate Vaccines ◽  
Accelerate Development ◽  
Freeze Dried ◽  
Modular Technology ◽  
Independent Distribution ◽  
Schu S4 ◽  
Engineered Bacteria

SummaryConjugate vaccines are among the most effective methods for preventing bacterial infections, representing a promising strategy to combat drug-resistant pathogens. However, existing manufacturing approaches limit access to conjugate vaccines due to centralized production and cold chain distribution requirements. To address these limitations, we developed a modular technology forin vitrobioconjugatevaccine expression (iVAX) in portable, freeze-dried lysates from detoxified, nonpathogenicEscherichia coli. Upon rehydration, iVAX reactions synthesize clinically relevant doses of bioconjugate vaccines against diverse bacterial pathogens in one hour. We show that iVAX synthesized vaccines against the highly virulent pathogenFranciscella tularensissubsp.tularensis(type A) strain Schu S4 elicited pathogen-specific antibodies in mice at significantly higher levels compared to vaccines produced using engineered bacteria. The iVAX platform promises to accelerate development of new bioconjugate vaccines with increased access through refrigeration-independent distribution and point-of-care production.

scholarly journals A Nontoxic Battery with 3D-Printed Housing for On-Demand Operation of Microcontrollers in Microfluidic Sensors

Micromachines ◽  
2019 ◽  
Vol 10 (9) ◽  
pp. 588 ◽  
Author(s):  
Kai Sachsenheimer ◽  
Christiane Richter ◽  
Dorothea Helmer ◽  
Frederik Kotz ◽  
Bastian Ernst Rapp
Keyword(s):  
Data Collection ◽  
Field Experiments ◽  
Point Of Care ◽  
Operation Mode ◽  
Lab On A Chip ◽  
Capacitive Sensor ◽  
Liquid Mixtures ◽  
On Demand ◽  
Care Systems ◽  
3D Printed

Microcontrollers have a low energy consumption and are convenient tools for the operation and readout of small lab-on-a-chip devices. The operation of microcontrollers for data collection and analysis is key for measurements and statistics in field experiments. However, for portable lab-on-a-chip or point-of-care systems in low-resource settings, the availability of energy sources is a bottleneck. Here, we present a simple, nontoxic aluminum/air redox battery with a 3D-printed housing for on-demand operation of a sensor using a microcontroller for data collection. The battery is stored in a dry state and can be manufactured conveniently using off-the-shelf components and a simple 3D printer. It can be quickly assembled and operates a microcontroller for at least one hour in continuous operation mode. We demonstrate its performance by collecting data from a capacitive sensor capable of determining the conductivity of liquid samples. Such sensors can be used for, e.g., determining the water quality or phase formation in liquid mixtures. The sensor performance in determining different conductivities of nonconductive and conductive liquids in droplets is demonstrated.

scholarly journals Magnetic Microdimer as Mobile Meter for Measuring Plasma Glucose and Lipids

2021 ◽  
Vol 9 ◽  
Author(s):  
Shimin Yu ◽  
Zhongqi Sun ◽  
Zhanxiang Zhang ◽  
Haoran Sun ◽  
Lina Liu ◽  
...  
Keyword(s):  
Plasma Glucose ◽  
Point Of Care ◽  
Testing System ◽  
Point Of Care Testing ◽  
Time Data ◽  
On Demand ◽  
Sensing Applications ◽  
Designed Materials ◽  
The Relationship ◽  
Surrounding Liquid

With the development of designed materials and structures, a wide array of micro/nanomachines with versatile functionalities are employed for specific sensing applications. Here, we demonstrated a magnetic propelled microdimer-based point-of-care testing system, which can be used to provide the real-time data of plasma glucose and lipids relying on the motion feedback of mechanical properties. On-demand and programmable speed and direction of the microdimers can be achieved with the judicious adjustment of the external magnetic field, while their velocity and instantaneous postures provide estimation of glucose, cholesterol, and triglycerides concentrations with high temporal accuracy. Numerical simulations reveal the relationship between motility performance and surrounding liquid properties. Such technology presents a point-of-care testing (POCT) approach to adapt to biofluid measurement, which advances the development of microrobotic system in biomedical fields.

“Aspirin - resistance”? A few critical considerations on definition, terminology, diagnosis, clinical value, natural course of atherosclerotic disease, and therapeutic consequences

VASA ◽  
2011 ◽  
Vol 40 (6) ◽  
pp. 429-438 ◽  
Author(s):  
Berent ◽  
Sinzinger
Keyword(s):  
Platelet Function ◽  
Point Of Care ◽  
Aspirin Resistance ◽  
Point Of View ◽  
Evidence Based ◽  
Platelet Function Tests ◽  
Clinical Value ◽  
Vascular Events ◽  
Therapeutic Consequences ◽  
Function Tests

Based upon various platelet function tests and the fact that patients experience vascular events despite taking acetylsalicylic acid (ASA or aspirin), it has been suggested that patients may become resistant to the action of this pharmacological compound. However, the term “aspirin resistance” was created almost two decades ago but is still not defined. Platelet function tests are not standardized, providing conflicting information and cut-off values are arbitrarily set. Intertest comparison reveals low agreement. Even point of care tests have been introduced before appropriate validation. Inflammation may activate platelets, co-medication(s) may interfere significantly with aspirin action on platelets. Platelet function and Cox-inhibition are only some of the effects of aspirin on haemostatic regulation. One single test is not reliable to identify an altered response. Therefore, it may be more appropriate to speak about “treatment failure” to aspirin therapy than using the term “aspirin resistance”. There is no evidence based justification from either the laboratory or the clinical point of view for platelet function testing in patients taking aspirin as well as from an economic standpoint. Until evidence based data from controlled studies will be available the term “aspirin resistance” should not be further used. A more robust monitoring of factors resulting in cardiovascular events such as inflammation is recommended.

Psychology 101, on demand

2008 ◽  
Author(s):  
Jamie Chamberlin
Keyword(s):  
On Demand
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