The Feeding Response of Rhodnius Prolixus to Blood Fractions, and the Role of ATP

1979 ◽  
Vol 78 (1) ◽  
pp. 225-232
Author(s):  
J.B. J. SMITH
Keyword(s):  
Adenosine Triphosphate ◽  
Rhodnius Prolixus ◽  
Human Erythrocytes ◽  
Erythrocyte Ghosts ◽  
Feeding Response ◽  
Sense Organs

Second-instar larvae of Rhodnius prolixus will gorge on washed human erythrocytes suspended in Ringer, but not on plasma, either with or without platelets. The ED 50 of erythrocytes is 4.5 vol of packed cells per 1000 vol of Ringer. Erythrocyte ‘ghosts’ are non-stimulatory, whereas the haemolysate induces gorging. Phagostimulation by ‘ghosts’ is restored by resealing them in 1 mM adenosine triphosphate. Evidence is presented that Rhodnius responds to blood via a release of ATP from a few erythrocytes close to the epipharyngeal sense organs.

scholarly journals Self-digestion of human erythrocyte membranes. Role of adenosine triphosphate and glutathione

1977 ◽  
Vol 164 (2) ◽  
pp. 469-472 ◽  
Author(s):  
A Brovelli ◽  
M Suhail ◽  
G Pallavicini ◽  
F Sinigaglia ◽  
C Balduini
Keyword(s):  
Chemical Composition ◽  
Adenosine Triphosphate ◽  
Human Erythrocyte ◽  
Incubation Medium ◽  
Reduced Glutathione ◽  
Human Erythrocytes ◽  
Erythrocyte Membranes ◽  
Digestion Process ◽  
Human Erythrocyte Membranes

Intact human erythrocytes incubated at 37 degrees C, pH7.4, release a sialoglycopeptide similar in its chemical composition, immunological and aggregation properties to the glycopeptide released by isolated ‘ghost’ membranes. The presence of ATP or reduced glutathione at physiological concentrations in the incubation medium of ‘ghost’ membranes inhibits this self-digestion process.

scholarly journals The Central Role of cAMP in Regulating Plasmodium falciparum Merozoite Invasion of Human Erythrocytes

2014 ◽  
Vol 10 (12) ◽  
pp. e1004520 ◽  
Author(s):  
Amrita Dawn ◽  
Shailja Singh ◽  
Kunal R. More ◽  
Faiza Amber Siddiqui ◽  
Niseema Pachikara ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Human Erythrocytes ◽  
Merozoite Invasion ◽  
Falciparum Merozoite

Lead and calcium transport in human erythrocyte

1999 ◽  
Vol 18 (5) ◽  
pp. 327-332 ◽  
Author(s):  
J V Calderón-Salinas ◽  
M A Quintanar-Escorcia ◽  
M T González-Martínez ◽  
C E Hernández-Luna
Keyword(s):  
Transport System ◽  
Human Erythrocyte ◽  
Calcium Transport ◽  
Human Erythrocytes ◽  
The Other ◽  
Erythrocyte Ghosts ◽  
Passive Transport ◽  
Ca Uptake

In this paper we report the lead (Pb) and calcium (Ca) uptake by erythrocyte ghosts. In both cases the transport was carried out by a passive transport system with two kinetic components (Michaelis-Menten and Hill). Pb and Ca were capable of inhibiting the transport of the other metal in a non-competitive way. Under hyperpolarization, the uptakes of Ca and Pb were enhanced and the Michaelis-Menten component prevailed. Both Ca and Pb uptakes were inhibited by N-ethyl-maleimide to the same extent. These results indicate that Pb and Ca share the same permeability pathway in human erythrocytes and that this transport system is electrogenic.

Insect peripheral nerves: accessibility of neurohaemal regions to lanthanum

1975 ◽  
Vol 18 (1) ◽  
pp. 179-197 ◽  
Author(s):  
N.J. Lane ◽  
R.A. Leslie ◽  
L.S. Swales
Keyword(s):  
Blood Brain Barrier ◽  
Peripheral Nerves ◽  
Rhodnius Prolixus ◽  
Ionic Lanthanum ◽  
Neurosecretory Axon ◽  
Thin Part ◽  
Free Media ◽  
Exogenous Substances

During incubation in vivo, exogenously applied ionic lanthanum comes to surround the numerous neurosecretory terminals which are found lying within or immediately beneath the acellular neural lamella ensheathing the nerves from fifth instar and adult specimens of Rhodnius prolixus. The lanthanum does not penetrate beyond the cellular perineurium, which completely surrounds the non-neurosecretory axons in these nerves and constitutes a form of ‘blood-brain barrier’. In some cases, however, lanthanum is found in the vicinity of a neurosecretory axon lying beneath the perineurium, where it can be assumed to have leaked in from the neurosecretory terminal lying free in the neural lamella. When nerves are incubated in calcium-free media, regions with an attenuated perineurium become ‘leaky’, in that lanthanum is found lying in those extracellular spaces between axons and glia which lie immediately below the thin part of the perineurial layer. Bathing solutions made slightly hyperosmotic to the haemolymph with sucrose have no apparent disruptive effects on the barrier. When the tissues are incubated in more hypertonic solutions, the perineurial barrier becomes ‘leaky’ throughout, and tracer pervades beyond its cells into all the intercellular spaced between glia and axons. The possible role of the zonulae occludentes in both the maintenance of the perineurial barrier and in the formation of interglial occlusions to local penetration of exogenous substances is considered.

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