The Effect of Environmental Calcium and Ovine Prolactin on Sodium Balance in Fundulus Kansae

1971 ◽  
Vol 55 (1) ◽  
pp. 63-75
Author(s):  
W. T. W. POTTS ◽  
W. R. FLEMING

1. The sodium contents and rates of turnover of sodium have been determined in both intact and hypophysectomized Fundulus kansae in a variety of media. 2. A reduction of the calcium content of fresh water from 1.0 to 0.1 mM/l increased the rate of sodium efflux by half while transter to calcium-free water increased the rate several fold. 3. The rate of sodium efflux increased fourfold in sea water containing no magnesium and only 1 mm/l calcium. 4. Hypophysectomy increased the rate of efflux in fresh water but reduced it in sea water. 5. Intact fish adapted to sea water and treated with prolactin showed a net gain of sodium probably due to a reduction in the activity of the sodium efflux pump. 6. Both intact and hypophysectomized fish on transfer from sea water to fresh water reduced the rate of efflux of sodium in a series of stages. 7. Studies of the rate of efflux of sodium before and after transfer between various media showed no evidence of exchange diffusion or of a linked sodium-potassium efflux pump, but did show evidence or interaction between sodium and chloride fluxes probably as a consequence of potential changes. 8. The rate of efflux of sodium from F. kansae doubled following hypophysectomy. The ability of hypophysectomized F. kansae to survive in fresh water after hypophysectomy, unlike F. heteroclitus, is due to a more effective uptake system.

2000 ◽  
Vol 203 (2) ◽  
pp. 389-396 ◽  
Author(s):  
S. Morris ◽  
P. Greenaway ◽  
A.M. Adamczewska ◽  
M.D. Ahern

The terrestrial robber crab Birgus latro L. regulates the composition of its final excretory product (termed P) depending on the availability of dietary salt by reabsorbing ions from urine passed over the gills. Laboratory and field-based studies investigated the nature of the mechanisms of control of this branchial ion uptake. B. latro were prepared such that their branchial chambers could be perfused with artificial urine, and the rate of ion transport from the artificial urine was determined. For B. latro acclimated to drinking fresh water, the rates of Na(+) and Cl(−) uptake were more than four times those of crabs drinking 70 % sea water. Crabs were injected with either saline carrier or the same solution containing either dopamine or dibutyryl cyclic AMP (db-cAMP) (final concentration 8.7×10(−)(7)mol l(−)(1)haemolymph). Dopamine and db-cAMP inhibited Na(+) and Cl(−) uptake in animals acclimated to fresh water and markedly reduced their gill Na(+)/K(+)-ATPase activity. Dopamine stimulated the production of cyclic AMP within the branchial epithelial cells. Dopamine, released from the pericardial organs, acts as a primary messenger, and cyclic AMP acts as a second messenger most likely promoting phosphorylation of membrane proteins. In contrast to aquatic brachyuran crabs, ion transport in B. latro, an anomuran, is controlled via an inhibitory effect. Terrestrial crabs normally have access to fresh water and must salvage salt from their urine, and a mechanism to down-regulate a normally active uptake system seems more appropriate to their ecology. Whether the control is stimulatory or inhibitory in the various air-breathing crabs may depend on the osmoregulatory abilities of their aquatic ancestors, but in either case has significant implications for the evolution of crustaceans to life on land. Further work must establish whether terrestrial brachyuran crabs are similar to B. latro and whether this crab is unique amongst the anomuran crabs.


1959 ◽  
Vol 36 (1) ◽  
pp. 157-176 ◽  
Author(s):  
J. SHAW

1. The mechanisms of salt and water balance in the East African fresh-water crab, Potamon niloticus, have been investigated. 2. The freezing-point depression of the blood is equivalent to that of a 271 mM./l. NaCl solution. 3. The animals cannot survive in solutions more concentrated than 75% sea water. Above the normal blood concentration, the blood osmotic pressure follows that of the medium. 4. The urine is iso-osmotic with the blood and is produced at a very slow rate. The potassium content is only half that of the blood. 5. The animal loses sodium at a rate of 8 µM./10 g./hr. mainly through the body surface. Potassium loss occurs at one-sixteenth of this rate. 6. Sodium balance can be maintained at a minimum external concentration of 0.05 mM./l. Potassium requires a concentration of 0.07 mM./l. 7. Active absorption of both sodium and potassium occurs. The rate of uptake of sodium depends on the extent of previous sodium loss. The rate of sodium uptake may be affected by such environmental factors as the salt content of the water, temperature and oxygen tension. 8. The normal oxygen consumption rate is 0.72 mg./10 g./hr. A minimum of 2.3% is used in doing osmotic work to maintain salt balance. 9. The salt and water balance in Potamon is discussed in relation to the adaptation of the Crustacea to fresh water. The importance of permeability changes is stressed.


1978 ◽  
Vol 235 (5) ◽  
pp. F444-F450 ◽  
Author(s):  
K. A. Kirchner ◽  
T. A. Kotchen ◽  
J. H. Galla ◽  
R. G. Luke

To evaluate the contribution of chloride to acute renin inhibition by sodium chloride, plasma renin activity (PRA) was measured before and after peripheral venous infusion of NaCl, NaHCO3, NaBr, NaNO3, lysine monohydrochloride, or lysine glutamate in NaCl-deprived rats. In contrast to controls and animals infused with other sodium salts, PRA decreased (P less than 0.01) after infusion with NaCl [from 28.3 +/- 2.8 to 13.3 +/- 1.8 ng/ml per h (SE)] and NaBr (from 40.6 +/- 6.2 to 21.8 +/- 3.9 ng/ml per h), and renal tubular halide reabsorption increased (P less than 0.05). Arterial pressure, plasma volume, inulin clearance, net sodium balance, serum Na+ and K+, and pH were not different among sodium-loaded groups. PRA was also suppressed (P less than 0.01) by infusion with lysine monohydrochloride (from 51.6 +/- 5.4 to 32.4 +/- 5.1 ng/ml per h) but not with lysine glutamate. These results suggest that inhibition of renin by sodium is dependent on an intrarenal effect of chloride. During infusion with sodium salts which suppressed renin, negative free water clearance (TcH2O) increased, whereas infusion with sodium salts that did not inhibit renin resulted in either no change or decreased TcH2O. The association of renin inhibition and increased TcH2O indirectly supports the hypothesis that renin suppression by chloride is related to the magnitude of absorptive chloride transport in the thick ascending limb of the loop of Henle.


Author(s):  
Olga Mashukova ◽  
Olga Mashukova ◽  
Yuriy Tokarev ◽  
Yuriy Tokarev ◽  
Nadejda Kopytina ◽  
...  

We studied for the first time luminescence characteristics of the some micromycetes, isolated from the bottom sediments of the Black sea from the 27 m depth. Luminescence parameters were registered at laboratory complex “Svet” using mechanical and chemical stimulations. Fungi cultures of genera Acremonium, Aspergillus, Penicillium were isolated on ChDA medium which served as control. Culture of Penicillium commune gave no light emission with any kind of stimulation. Culture of Acremonium sp. has shown luminescence in the blue – green field of spectrum. Using chemical stimulation by fresh water we registered signals with luminescence energy (to 3.24 ± 0.11)•108 quantum•cm2 and duration up to 4.42 s, which 3 times exceeded analogous magnitudes in a group, stimulated by sea water (p < 0.05). Under chemical stimulation by ethyl alcohol fungi culture luminescence was not observed. Culture of Aspergillus fumigatus possessed the most expressed properties of luminescence. Stimulation by fresh water culture emission with energy of (3.35 ± 0.11)•108 quantum•cm2 and duration up to 4.96 s. Action of ethyl alcohol to culture also stimulated signals, but intensity of light emission was 3–4 times lower than under mechanical stimulation. For sure the given studies will permit not only to evaluate contribution of marine fungi into general bioluminescence of the sea, but as well to determine places of accumulation of opportunistic species in the sea.


2018 ◽  
Vol 3 (2) ◽  
pp. 38-47
Author(s):  
Muhammad Abdul Azis ◽  
Nuryake Fajaryati

This research aims to create a Reosquido desalination tool for evaporation methods using a microcontroller. This tool can control the temperature to speed up the evaporation process in producing fresh water. The method applied to Reosquido desalination uses Evaporation. The first process before evaporation is the detection of temperature in sea water that will be heated using an element heater. The second process of temperature measurement is to turn off and turn on the Arduino Uno controlled heater, when the temperature is less than 80 ° then the heater is on. The third process is evaporation during temperatures between 80 ° to 100 °, evaporation water sticks to the glass roof which is designed by pyramid. Evaporated water that flows into the reservoir is detected by its solubility TDS value. The fourth process is heater off when the temperature is more than 100 °. Based on the results of the testing, the desalination process using a microcontroller controlled heater can speed up the time up to 55% of the previous desalination process tool, namely manual desalination prsoes without using the heater element controlled by the temperature and controlled by a microcontroller which takes 9 hours. Produces fresh water as much as 30ml from 3000ml of sea water, so that it can be compared to 1: 100.


2014 ◽  
Vol 592-594 ◽  
pp. 2409-2415 ◽  
Author(s):  
S. Naga Sarada ◽  
Banoth Hima Bindu ◽  
Sri Rama R. Devi ◽  
Ravi Gugulothu

In recent years with the exacerbation of energy shortage, water crisis increases around the world. With the continuous increase in the level of greenhouse gas emissions, the use of various sources of renewable energy is increasingly becoming important for sustainable development. Due to the rising oil price and environmental regulations, the demand of utilizing alternative power sources increased dramatically. Alternative energy and its applications have been heavily studied for the last decade. Energy and water are essential for mankind that influences the socioeconomic development of any nation. Pure water resources become more and more scarce every day as rivers, lakes wells and even seawater pollution rapidly increases. Solar energy is one promising solution to secure power and potable water to future generation. The process of distillation can be used to obtain fresh water from salty, brackish or contaminated water. Water is available in different forms such as sea water, underground water, surface water and atmospheric water. Clean water is essential for good health. The search for sustainable energy resources has emerged as one of the most significant and universal concerns in the 21st century. Solar energy conversion offers a cost effective alternative to our traditional usages. Solar energy is a promising candidate in many applications. Among the alternative energy sources used for electricity production, wind and solar energy systems have become more attractive in recent years. For areas where electricity was not available, stand alone wind and solar systems have been increasingly used. The shortage of drinking water in many countries throughout the world is a serious problem. Humankind has depended for ages on river, sea water and underground water reservoirs for its fresh water needs. But these sources do not always prove to be useful due to the presence of excessive salinity in the water. To resolve this crisis, different methods of solar desalination have been used in many countries. Distillation is a well known thermal process for water purification, most importantly, water desalination. Most of the conventional water distillation processes are highly energy consuming and require fossil fuels as well as electric power for their operation. Single basin solar still is a popular solar device used for converting available brackish or waste water into potable water. Because of its lower productivity, it is not popularly used. Numbers of works are under taken to improve the productivity and efficiency of the solar still. There are large numbers of PCMs that melt and solidify at wide range of temperatures, making them attractive in a number of applications. PCMs have been widely used in latent heat thermal storage systems for heat pumps, solar engineering and spacecraft thermal control applications. The use of PCMs for heating and cooling applications for buildings has been investigated within the past decade. The experimental results computed in the field of water distillation process using solar energy in the presence of energy storage materials sodium sulphate and sodium acetate are discussed in this paper. Keywords: solar energy, saline water, distillation, phase change material.


1996 ◽  
Vol 199 (11) ◽  
pp. 2467-2475 ◽  
Author(s):  
M Vijayan ◽  
J Morgan ◽  
T Sakamoto ◽  
E Grau ◽  
G Iwama

We tested the hypothesis that nutritional state affects seawater acclimation by transferring either fed or food-deprived (2 weeks) male tilapia (Oreochromis mossambicus) from fresh water to full-strength sea water. Food-deprivation resulted in a significant increase in plasma concentrations of Na+, Cl-, cortisol, glucose, total amino acid, glutamate, serine and alanine, and in hepatic pyruvate kinase (PK) and lactate dehydrogenase (LDH) activities, whereas the prolactin-188 to prolactin-177 ratio (tPRL188:tPRL177) and plasma prolactin-188 (tPRL188), lactate, arginine and hepatic glycogen content and hepatic alanine aminotransferase (AlaAT) and 3-hydroxyacyl-Coenzyme A dehydrogenase (HOAD) activities were lower than in the fed group. Seawater transfer significantly increased the tPRL188:tPRL177 ratio and plasma concentrations of Na+, Cl-, K+, growth hormone (GH), glucose, aspartate, tyrosine, alanine, methionine, phenylalanine, leucine, isoleucine and valine levels as well as gill Na+/K+-ATPase activity and hepatic PK and LDH activities, whereas plasma tPRL177, tPRL188, glycine and lysine concentrations were significantly lower than in fish retained in fresh water. There was a significant interaction between nutritional state and salinity that affected the tPRL188:tPRL177 ratio and plasma concentrations of Cl-, GH, glucose, aspartate, tyrosine, serine, alanine, glycine, arginine and hepatic PK, LDH, AlaAT, aspartate aminotransferase, glutamate dehydrogenase and HOAD activities. These results, taken together, indicate that food-deprived fish did not regulate their plasma Cl- levels, despite an enhancement of plasma hormonal and metabolic responses in sea water. Our study also suggests the possibility that plasma prolactin and essential amino acids may be playing an important role in the seawater acclimation process in tilapia.


Author(s):  
OO Sinitsyna ◽  
VV Turbinsky ◽  
TM Ryashentseva ◽  
EP Lavrik

Background. Uneven distribution of fresh water sources on the land surface encourages a search for effective techniques of potable water preparation by desalination of seawater. Hygienic issues of such desalination methods as distillation, reverse osmosis, electrodialysis, and ion exchange have been investigated by now and appropriate limitations, requirements, and additional measures to ensure safety of desalinated drinking water have been established. Objective. To summarize and systematize the results of studying characteristics of various methods of seawater desalination for its further use for drinking and household purposes. Materials and methods. We conducted a systematic review of studies published in Russian and in English, found in the PubMed and Web of Science databases, and selected 40 literary sources containing an empirical assessment of effectiveness of seawater desalination and preparation of drinking water. We also scrutinized regulatory documents and guidelines of domestic sanitary legislation. The research results were systematized by the main desalination methods. Results and discussion. We established that the use of seawater for the preparation of fresh water for drinking and household purposes is becoming increasingly widespread around the world. Drinking water obtained from seawater, in all cases, requires additional treatment and measures to optimize its mineral composition and protect against microorganisms. Conclusion. The main challenges of ensuring sanitary and epidemiological wellbeing of the population when using desalinated seawater for drinking and household purposes include selection of a source, arrangement of sites of water intake properly protected from natural and man-made pollution, substantiation of techniques and modes of preliminary preparation of source seawater adequate to its composition, basic desalination, ensuring safety of products of destruction and migration of toxic substances from reagents and materials of desalination plants, additional conditioning with the necessary elements and disinfection of the prepared water, as well as environmental protection from desalination waste.


1968 ◽  
Vol 48 (2) ◽  
pp. 359-380
Author(s):  
D. W. SUTCLIFFE

1. Sodium uptake and loss rates are given for three gammarids acclimatized to media ranging from fresh water to undiluted sea water. 2. In Gammarus zaddachi and G. tigrinus the sodium transporting system at the body surface is half-saturated at an external concentration of about 1 mM/l. and fully saturated at about 10 mM/l. sodium. In Marinogammarus finmarchicus the respective concentrations are six to ten times higher. 3. M. finmarchicus is more permeable to water and salts than G. zaddachi and G. tigrinus. Estimated urine flow rates were equivalent to 6.5% body weight/hr./ osmole gradient at 10°C. in M. finmarchicus and 2.8% body weight/hr./osmole gradient in G. zaddachi. The permeability of the body surface to outward diffusion of sodium was four times higher in M. finmarchicus, but sodium losses across the body surface represent at least 50% of the total losses in both M. finmarchicus and G. zaddachi. 4. Calculations suggest that G. zaddachi produces urine slightly hypotonic to the blood when acclimatized to the range 20% down to 2% sea water. In fresh water the urine sodium concentration is reduced to a very low level. 5. The process of adaptation to fresh water in gammarid crustaceans is illustrated with reference to a series of species from marine, brackish and freshwater habitats.


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