A Study on the Mechanism of Cleavage in the Amphibian Egg

1963 ◽  
Vol 40 (1) ◽  
pp. 7-14
Author(s):  
K. DAN ◽  
M. KUNO KOJIMA

1. In the eggs of the salamander, Triturus pyrrhogaster, there is an area extending about 1 mm. ahead of the advancing tip of the cleavage furrow where preparation for furrow formation is going on. 2. Severance of this area ahead of an advancing furrow tip does not stop the progress of the furrow. The furrow continues from this area. 3. The cleavage furrow of Triturus pyrrhogaster eggs advances strictly along a straight line. 4. A cut made across its path ahead of a furrow is healed as the furrow tip approaches sufficiently to allow its straight passage. 5. An excised piece of cortex which includes material taken from within 1 mm. of an advancing furrow tip will form a furrow and divide.

1974 ◽  
Vol 60 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Tsuyoshi Sawai ◽  
Mitsuki Yoneda

In the eggs of the newt, Cynops (Triturus) pyrrhogaster, change in stiffness of the cortex was measured in various regions at the time of the cleavage. Measurements were performed by Mitchison and Swann's cell elastimeter method with a modification, in which two fine pipettes were attached to the surface of one egg at the same time, in order to compare the rigidity of two regions. The stiffness of the cortex changed very little before the start of the first cleavage. However, just before the appearance of the first cleavage furrow, the stiffness increased rapidly at the animal pole region, which later returned to the former level. As the cleavage furrow progressed, a wave of high stiffness travelled meridionally as a belt along the surface from the animal pole region toward the vegetal region. At second cleavage, the cycle of change in stiffness was repeated.


1972 ◽  
Vol 11 (2) ◽  
pp. 543-556
Author(s):  
TSUYOSHI SAWAI

In the eggs of the newt, Triturus pyrrhogaster, 2 separate factors are recognized which take part in cleavage furrow formation. (1) The inductive capacity for the furrow formation by the cytoplasm lying under the cortex along the cleavage furrow (FIC); and (2) the reactivity of the overlying cortex to form a furrow in response to FIC. (1) FIC. The inductive capacity is shown by the fact that FIC induces a furrow on whichever part of the surface under which FIC is transplanted. FIC is distributed along the cleavage furrow and even extends along the future furrow plane ahead of the furrow tip. The distance FIC precedes the furrow tip is about 1.0 mm in the animal hemisphere and is less in the vegetal hemisphere. In the direction at right angles to the furrow plane, FIC does not spread more than 0.1 mm. FIC is also present in the eggs of Xenopus laevis. Species specificity of FIC for induction is not found between Triturus and Xenopus. (2) Surface layer. At the onset of the first cleavage, the reactivity of the cortex to form the furrow in answer to FIC induction is localized on the animal pole region. The reactivity of the cortex propagates medially as a belt along the surface towards the vegetal pole with the advancing tip of the cleavage furrow. After the furrow is completed, the reactivity begins to be lost from the animal pole region, and eventually over the entire surface. The reactivity, however, reappears on the animal pole region simultaneously with the second cleavage.


Author(s):  
D.R. Ensor ◽  
C.G. Jensen ◽  
J.A. Fillery ◽  
R.J.K. Baker

Because periodicity is a major indicator of structural organisation numerous methods have been devised to demonstrate periodicity masked by background “noise” in the electron microscope image (e.g. photographic image reinforcement, Markham et al, 1964; optical diffraction techniques, Horne, 1977; McIntosh,1974). Computer correlation analysis of a densitometer tracing provides another means of minimising "noise". The correlation process uncovers periodic information by cancelling random elements. The technique is easily executed, the results are readily interpreted and the computer removes tedium, lends accuracy and assists in impartiality.A scanning densitometer was adapted to allow computer control of the scan and to give direct computer storage of the data. A photographic transparency of the image to be scanned is mounted on a stage coupled directly to an accurate screw thread driven by a stepping motor. The stage is moved so that the fixed beam of the densitometer (which is directed normal to the transparency) traces a straight line along the structure of interest in the image.


Author(s):  
Joseph A. Zasadzinski

At low weight fractions, many surfactant and biological amphiphiles form dispersions of lamellar liquid crystalline liposomes in water. Amphiphile molecules tend to align themselves in parallel bilayers which are free to bend. Bilayers must form closed surfaces to separate hydrophobic and hydrophilic domains completely. Continuum theory of liquid crystals requires that the constant spacing of bilayer surfaces be maintained except at singularities of no more than line extent. Maxwell demonstrated that only two types of closed surfaces can satisfy this constraint: concentric spheres and Dupin cyclides. Dupin cyclides (Figure 1) are parallel closed surfaces which have a conjugate ellipse (r1) and hyperbola (r2) as singularities in the bilayer spacing. Any straight line drawn from a point on the ellipse to a point on the hyperbola is normal to every surface it intersects (broken lines in Figure 1). A simple example, and limiting case, is a family of concentric tori (Figure 1b).To distinguish between the allowable arrangements, freeze fracture TEM micrographs of representative biological (L-α phosphotidylcholine: L-α PC) and surfactant (sodium heptylnonyl benzenesulfonate: SHBS)liposomes are compared to mathematically derived sections of Dupin cyclides and concentric spheres.


Author(s):  
Norman L. Dockum ◽  
John G. Dockum

Ultrastructural characteristics of fractured human enamel and acid-etched enamel were compared using acetate replicas shadowed with platinum and palladium. Shadowed replications of acid-etched surfaces were also obtained by the same method.Enamel from human teeth has a rod structure within which there are crystals of hydroxyapatite contained within a structureless organic matrix composed of keratin. The rods which run at right angles from the dentino-enamel junction are considered to run in a straight line perpendicular to the perimeter of the enamel, however, in many areas these enamel rods overlap, interlacing and intertwining with one another.


Author(s):  
Akane SETO ◽  
Aleksandar SHURBEVSKI ◽  
Hiroshi NAGAMOCHI ◽  
Peter EADES

1979 ◽  
Vol 7 (1) ◽  
pp. 31-39
Author(s):  
G. S. Ludwig ◽  
F. C. Brenner

Abstract An automatic tread gaging machine has been developed. It consists of three component systems: (1) a laser gaging head, (2) a tire handling device, and (3) a computer that controls the movement of the tire handling machine, processes the data, and computes the least-squares straight line from which a wear rate may be estimated. Experimental tests show that the machine has good repeatability. In comparisons with measurements obtained by a hand gage, the automatic machine gives smaller average groove depths. The difference before and after a period of wear for both methods of measurement are the same. Wear rates estimated from the slopes of straight lines fitted to both sets of data are not significantly different.


1979 ◽  
Vol 7 (1) ◽  
pp. 3-13
Author(s):  
F. C. Brenner ◽  
A. Kondo

Abstract Tread wear data are frequently fitted by a straight line having average groove depth as the ordinate and mileage as the abscissa. The authors have observed that the data points are not randomly scattered about the line but exist in runs of six or seven points above the line followed by the same number below the line. Attempts to correlate these cyclic deviations with climatic data failed. Harmonic content analysis of the data for each individual groove showed strong periodic behavior. Groove 1, a shoulder groove, had two important frequencies at 40 960 and 20 480 km (25 600 and 12 800 miles); Grooves 2 and 3, the inside grooves, had important frequencies at 10 240, 13 760, and 20 480 km (6400, 8600, and 12 800 miles), with Groove 4 being similar. A hypothesis is offered as a possible explanation for the phenomenon.


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