Some Observations on Water-Soluble Factors Which Accelerate the Growth in Aseptic Culture of Larvae of Drosophila Melanogaster

1956 ◽  
Vol 33 (1) ◽  
pp. 142-154
Author(s):  
M. BEGG
Keyword(s):  
Drosophila Melanogaster ◽  
Basal Medium ◽  
Water Soluble ◽  
Soluble Form ◽  
Active Components ◽  
Reduced Pressure ◽  
Aseptic Culture ◽  
Heat Stable ◽  
Anion Exchange Resins ◽  
Exchange Resins

1. An effective technique is described for the aseptic culture of larvae of Drosophila melanogaster. 2. Factors which accelerate the growth of Drosophila in aseptic culture on a basal medium containing casein as a source of amino acids can be extracted from both yeast and pancreas in water-soluble form. 3. Part of the activity of such fractions can be removed by adsorption on anion exchange resins, or on charcoal; and eluted with ammonia. 4. The whole activity is dialysable and recoverable from the dialysate by evaporation under reduced pressure. 5. The active components are heat stable. 6. The active fraction is insoluble in N-butanol, ethanol, acetone, pyridine and ether. 7. Some evidence is presented for the presence of at least two components. 8. The possibility that one of the factors may be a peptide is considered. It is thought unlikely that it is an amino acid.

Properties of conjugation hormones (erogens) from the basidiomycete Tremella mesenteria

1974 ◽  
Vol 52 (3) ◽  
pp. 521-524 ◽  
Author(s):  
Ian D. Reid
Keyword(s):  
Mating Type ◽  
Active Material ◽  
The Other ◽  
Active Components ◽  
Small Peptides ◽  
Isolation And Identification ◽  
Polar Solvents ◽  
Anion Exchange Resins ◽  
Polystyrene Resin ◽  
Exchange Resins

Hormones produced by haploid cells of one mating type of T. mesenterica and inducing conjugation tubes in the other mating type have been studied in preparation for isolation and identification. The active material can be extracted from aqueous solution with n-butanol, but not with less polar solvents, and is adsorbed on cation and anion exchange resins, charcoal, and neutral polystyrene resin. The molecular weight is probably less than 1000. Three active components can be separated by silica gel column chromatography with a gradient of water in ethanol, or by paper chromatography. The conjugation hormones may be non-polar amino acids or small peptides.

scholarly journals Characteristics of Heat Stable Salts Treatment Using Anion Exchange Resins in CO2Absorption Process

2015 ◽  
Vol 21 (1) ◽  
pp. 22-32 ◽  
Author(s):  
Kyung-Bin Park ◽  
Jun-Hyoung Cho ◽  
Soo-Bin Jeon ◽  
You-Young Lim ◽  
Kwang-Joong OH
Keyword(s):  
Anion Exchange ◽  
Heat Stable ◽  
Anion Exchange Resins ◽  
Exchange Resins

Water-soluble constituents of fruit. II. The separation of acids on anion-exchange resins: the isolation of l-auinic acid from Apricots

1955 ◽  
Vol 8 (2) ◽  
pp. 267
Author(s):  
EFLJ Anet ◽  
TM Reynolds
Keyword(s):  
Organic Acids ◽  
Silver Nitrate ◽  
Anion Exchange ◽  
Water Soluble ◽  
Hydroxy Acids ◽  
Anion Exchange Resins ◽  
Rf Values ◽  
Solvent Systems ◽  
Basic Anion ◽  
Exchange Resins

Displacement chromatography on columns of strongly basic anion-exchange resins has been used to separate, isolate, and purify some water-soluble organic acids. The order of emergence of 27 acids from these columns is recorded. Using this method pure l-quinic, succinic, L-malic, and citric acids were isolated from the flesh of the apricot fruit. The method was also used for the purification of hydroxy-acids. The detection of acids on paper chromatograms with a silver nitrate reagent is described ; silver nitrate-sodium hydroxide was used to detect acids having a vic.-glycol or α-keto group. The RF values for 30 acids in two solvent systems are recorded.

Water-soluble constituents of fruit. IV. The organic acids in peaches

1955 ◽  
Vol 8 (2) ◽  
pp. 280 ◽  
Author(s):  
EFLJ Anet ◽  
TM Reynolds
Keyword(s):  
Citric Acid ◽  
Organic Acids ◽  
Anion Exchange ◽  
Galacturonic Acid ◽  
Water Soluble ◽  
Mucic Acid ◽  
Immature Fruit ◽  
Anion Exchange Resins ◽  
Basic Anion ◽  
Exchange Resins

The acids in several varieties of peaches were separated by displacement chromatography on strongly basic anion-exchange resins. l-Quinic, L-malic, and citric acids were the three main acids, any one of them being the predominant acid depending on the variety, season, and maturity of the fruit. Mucic acid was found in small quantity in all samples ; galacturonic acid was only present in fruit picked at commercial maturity and ripened at 20 �C. The effect of maturity on the three major acids was studied for one crop of Blackburn Elberta peaches; the immature fruit contained only traces of citric acid.

Removal of Hydrophobic and Hydrophilic Constituents by Anion Exchange Resin

1999 ◽  
Vol 40 (9) ◽  
pp. 207-214 ◽  
Author(s):  
J.-P. Croué ◽  
D. Violleau ◽  
C. Bodaire ◽  
B. Legube
Keyword(s):  
Molecular Weight ◽  
Anion Exchange ◽  
Water Source ◽  
Atomic Ratio ◽  
Size Exclusion ◽  
Salting Out ◽  
Anion Exchange Resins ◽  
Hydrophilic Character ◽  
Exchange Resins

The objective of this work was to compare the affinity of well characterized NOM fractions isolated from two surface waters with strong (gel matrix and macroporous matrix) and weak anion exchange resins (AER) using batch experiment conditions. The structural characterization of the fraction of NOM has shown that the higher the hydrophilic character, the lower the C/O atomic ratio, the lower the SUVA, the lower the aromatic carbon content and the lower the molecular weight. In general (not always), strong AER was more efficient to remove DOC than weak AER. For the same water source (Suwannee River), the higher the molecular weight of the NOM fraction, the lower the affinity with AER. Increasing the ionic strength favored the removal of the hydrophobic NOM fraction (“salting out” effect) while increasing the pH apparently reduced the removal of the hydrophilic NOM fraction. Results were discussed in terms of size exclusion, adsorption, anion exchange and also hydrophobic/hydrophilic repulsion.

scholarly journals A GENETICALLY DISTINCT FORM OF CYCLIC AMP PHOSPHODIESTERASE ASSOCIATED WITH CHROMOMERE 3D4 IN DROSOPHILA MELANOGASTER

Genetics ◽  
1979 ◽  
Vol 91 (3) ◽  
pp. 521-535
Author(s):  
John A Kiger ◽  
Eric Golanty
Keyword(s):  
Drosophila Melanogaster ◽  
Cyclic Amp ◽  
Structural Gene ◽  
Regulatory Gene ◽  
Heat Stability ◽  
Normal Female ◽  
Dependent Manner ◽  
Cyclic Amp Phosphodiesterase ◽  
Heat Stable ◽  
Camp Levels

ABSTRACT Two cyclic AMP phosphodiesterase enzymes (E.C.3.1.4.17) are present in homogenates of adult Drosophila melanogaster. The two enzymes differ from one another in heat stability, affinity for Mg++, Ca++ activation and molecular weight. They do not differ markedly in their affinities for cyclic AMP, and both exhibit anomalous Michaelis-Menten kinetics. The more heatlabile enzyme is controlled in a dosage-dependent manner by chromomere 3D4 of the X chromosome and is absent in flies that are deficient for chromomere 3D4. Chromomere 3D4 is also necessary for the maintenance of normal cAMP levels, for male fertility, and for normal female fertility and oogenesis. The structural gene(s) for the more heat-stable enzyme is located outside of chromomeres 3C12-3D4. Whether 3D4 contains a structural gene, or a regulatory gene necessary for the presence of the labile enzyme, remains to be determined.

scholarly journals Analyzing the Carotenoid Composition of Melilot (Melilotus officinalis (L.) Pall.) Extracts and the Effects of Isolated (All-E)-lutein-5,6-epoxide on Primary Sensory Neurons and Macrophages

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 503
Author(s):  
Györgyi Horváth ◽  
Eszter Csikós ◽  
Eichertné Violetta Andres ◽  
Tímea Bencsik ◽  
Anikó Takátsy ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Sensory Neurons ◽  
Biological Activities ◽  
Water Soluble ◽  
Soluble Form ◽  
Primary Sensory Neurons ◽  
Isolation And Identification ◽  
Melilotus Officinalis ◽  
Carotenoid Composition ◽  
Anti Inflammatory

Melilotus officinalis is known to contain several types of secondary metabolites. In contrast, the carotenoid composition of this medicinal plant has not been investigated, although it may also contribute to the biological activities of the drug, such as anti-inflammatory effects. Therefore, this study focuses on the isolation and identification of carotenoids from Meliloti herba and on the effect of isolated (all-E)-lutein 5,6-epoxide on primary sensory neurons and macrophages involved in nociception, as well as neurogenic and non-neurogenic inflammatory processes. The composition of the plant extracts was analyzed by high performance liquid chromatography (HPLC). The main carotenoid was isolated by column liquid chromatography (CLC) and identified by MS and NMR. The effect of water-soluble lutein 5,6-epoxide-RAMEB (randomly methylated-β-cyclodextrin) was investigated on Ca2+-influx in rat primary sensory neurons induced by the activation of the transient receptor potential ankyrin 1 receptor agonist to mustard-oil and on endotoxin-induced IL-1β release from isolated mouse peritoneal macrophages. (all-E)-Lutein 5,6-epoxide significantly decreased the percent of responsive primary sensory neurons compared to the vehicle-treated stimulated control. Furthermore, endotoxin-evoked IL-1β release from macrophages was significantly decreased by 100 µM lutein 5,6-epoxide compared to the vehicle-treated control. The water-soluble form of lutein 5,6-epoxide-RAMEB decreases the activation of primary sensory neurons and macrophages, which opens perspectives for its analgesic and anti-inflammatory applications.

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