scholarly journals Refinements in Polarized Light Microscopy

1950 ◽  
Vol 27 (2) ◽  
pp. 226-237
Author(s):  
M. M. SWANN ◽  
J. M. MITCHISON
Keyword(s):  
Light Microscopy ◽  
Wave Length ◽  
Polarized Light ◽  
Bright Light ◽  
Visual Observation ◽  
Polarized Light Microscopy ◽  
Light Sources ◽  
Photographic Method ◽  
Biological Objects ◽  
Mica Plate

Various measures to increase the sensitivity of the polarizing microscope for visual observation are discussed. These include choice of polarizing apparatus, reducing condenser and objective aperture, reducing lamp iris, blooming lenses, use of mica plate compensators and use of bright light sources. By such means as these it is possible to detect retardations down to 0.28 A., or 1/20,000th of a wave-length. A photographic method for measuring retardations down to the same limit in small biological objects, is also described. We are deeply indebted to Mr E. W. Taylor of Messrs Cooke, Troughton and Simms Ltd., for his great help in fitting our microscope with double Polaroids and a graduated compensator mount, as well as for blooming our lenses and making special low retardation mica plate compensators. We are also indebted to Dr L. E. R. Picken and Dr A. F. Hallimond for helpful suggestions at various stages of the work.

Ferroelastic domains and phase transitions in organic–inorganic hybrid perovskite CH3NH3PbBr3

2021 ◽  
Author(s):  
Maryam Bari ◽  
Alexei A. Bokov ◽  
Zuo-Guang Ye
Keyword(s):  
Phase Transitions ◽  
Electric Field ◽  
Light Microscopy ◽  
Domain Structure ◽  
Polarized Light ◽  
External Stress ◽  
Polarized Light Microscopy ◽  
Inorganic Hybrid ◽  
Hybrid Perovskite ◽  
Ferroelastic Domains

Polarized light microscopy reveals twin domains and symmetry of the phases in CH3NH3PbBr3 crystal; domain structure remains unresponsive to electric field but changes under external stress, confirming ferroelasticity while ruling out ferroelectricity.

scholarly journals Exploiting the Binocular Head in Polarized Light Microscopy

1994 ◽  
Vol 2 (4) ◽  
pp. 16-16
Author(s):  
Walter C. McCrone
Keyword(s):  
Light Microscopy ◽  
Polarized Light ◽  
Polarized Light Microscopy ◽  
The Other ◽  
Monocular Viewing ◽  
Area Of Interest

Having been brought up on monocular microscopes I find the omnipresent binocular systems a luxury. To support this viewpoint I'd like to suggest some benefits you may not have considered.Because I'm used to monocular viewing I sometimes use two different oculars, say 10X and 25X, in order to scan quickly to find an area of interest and then to examine the detail with higher magnification. Occasionally I use both oculars simultaneously and “concentrate” on either image to the exclusion of the other. A better way is to set the interocular distance at the extreme setting most different from your own interocular distance. By moving your head about a centimeter either way you can use either ocular.

Structural Morphology of Rabbit Patella and Suprapatella Cartilage by Microscopic MRI and Polarized Light Microscopy

Cartilage ◽  
2021 ◽  
pp. 194760352199088
Author(s):  
Hannah Mantebea ◽  
Syeda Batool ◽  
Mouhamad Hammami ◽  
Yang Xia
Keyword(s):  
Articular Cartilage ◽  
Light Microscopy ◽  
Design Method ◽  
Strong Dependence ◽  
Polarized Light ◽  
Polarized Light Microscopy ◽  
Structural Morphology ◽  
Pixel Resolution ◽  
Patellar Cartilage ◽  
Quantitative Results

Objective In order to appreciate the roles articular cartilage of sesamoid bones and sesamoid fibrocartilage play in anatomy and pathology, the articular cartilage of the patella ( n = 4) and suprapatella ( n = 4) (a sesamoid fibrocartilage) of 12 to 14 weeks old New Zealand rabbits were studied qualitatively and quantitatively. Design/Method The intact knee joints and block specimens from the joints were imaged using microscopic magnetic resonance imaging (µMRI) at a 97.6-µm pixel resolution for the former and 19.5-µm resolution for the latter. Histological sections were made out of the µMRI-imaged specimens, which were imaged using polarized light microscopy (PLM) at 0.25-, 1-, and 4-µm pixel resolutions. Results The patella cartilage varied in thickness across the medial to lateral ends of the sesamoid bone with the central medial aspect slightly thicker than the lateral aspect. The suprapatella fibrocartilage decreased proximally away from the knee joint. Quantitative results of patellar cartilage showed strong dependence of fiber orientation with the tissue depth. Three histological zones can be clearly observed, which are similar to articular cartilage from other large animals. The sesamoid fibrocartilage has one thin surface layer (10 µm thick) of parallel-arranged structured fibers followed immediately by the majority of random fibers in bulk tissue. T2 relaxation time anisotropy was observed in the patellar cartilage but not in the bulk fibrocartilage. Conclusion Given the different functions of these 2 different types of cartilages in joint motion, these quantitative results will be beneficial to future studies of joint diseases using rabbits as the animal model.

High-Resolution Fiber and Fiber Tract Imaging Using Polarized Light Microscopy in the Human, Monkey, Rat, and Mouse Brain

2016 ◽  
pp. 369-389 ◽  
Author(s):  
Karl Zilles ◽  
Nicola Palomero-Gallagher ◽  
David Gräßel ◽  
Philipp Schlömer ◽  
Markus Cremer ◽  
...  
Keyword(s):  
High Resolution ◽  
Light Microscopy ◽  
Mouse Brain ◽  
Polarized Light ◽  
Polarized Light Microscopy ◽  
Fiber Tract

An in vitro Evaluation of Artificial Caries-like Lesions on Restored Overdenture Abutments

1988 ◽  
Vol 67 (3) ◽  
pp. 582-584 ◽  
Author(s):  
P.P. Kambhu ◽  
R.L. Ettinger ◽  
J.S. Wefel
Keyword(s):  
Light Microscopy ◽  
Polarized Light ◽  
Polarized Light Microscopy ◽  
The Other ◽  
Type Ii ◽  
Restorative Materials ◽  
Abutment Teeth ◽  
The Mean ◽  
Materials Used

An acidified dialyzed gelatin gel system was used to determine the caries resistance of a variety of restorative materials used to obturate the canal orifice of overdenture abutment teeth. The restorative materials used were Tytin, Tytin + Copalite, P30 + Scotchbond, Fuji Ionomer-Type II, and Miracle Mix. Polarized light microscopy and microradiography were used to examine the caries-like lesions adjacent to the restorations. The lesions formed in the Fuji Ionomer-Type II and Miracle Mix groups appeared arrested at the wall adjacent to the restoration, and did not penetrate apically down the wall as did those associated with the other restorative materials. The mean depths of lesions adjacent to Fuji Ionomer-Type II and Miracle Mix restorations were significantly less than those of Tytin, Tytin + Copalite, or P30 + Scotchbond.

Sign in / Sign up

Export Citation Format

Share Document