scholarly journals Role of the G protein-coupled receptors GPR84 and GPR119 in the central regulation of food intake in rainbow trout

2021 ◽  
Author(s):  
Cristina Velasco ◽  
Marta Conde-Sieira ◽  
Sara Comesaña ◽  
Mauro Chivite ◽  
Jesús M. Míguez ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Food Intake ◽  
Mrna Abundance ◽  
Signalling Pathways ◽  
Fish Brain ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Food Intake Regulation ◽  
Intake Regulation ◽  
The Impact

We evaluated the role of GPR84 and GPR119 in food intake regulation in fish using rainbow trout (Oncorhynchus mykiss) as a model. In a first experiment, we assessed the effects on food intake of intracerebroventricular treatment with agonists of these receptors. In a second experiment, we assessed in hypothalamus and hindbrain the impact of the same treatments on mRNA abundance of neuropeptides involved in the metabolic control of food intake (npy, agrp1, pomca1, and cartpt) as well as in changes in parameters related to signalling pathways and transcription factors involved in the integrative response leading to neuropeptide production. Treatment with both agonists elicited an anorectic response in rainbow trout attributable to changes observed in the mRNA abundance of the four neuropeptides. Changes in neuropeptides relate to changes observed in mRNA abundance and phosphorylation status of the transcription factor Foxo1. These changes occurred in parallel with changes in phosphorylation status of Ampkα and Akt, mRNA abundance of mTOR as well as in signalling pathways related to PLCβ and IP3. These results allow us to suggest that 1) at least part of the capacity of fish brain to sense MCFA like octanoate depends on the function of GPR84, and 2) the capacity of fish brain to sense NAE or triglyceride-derived molecules through binding of these ligands to GPR119.

scholarly journals The long-chain fatty acid receptors FFA1 and FFA4 are involved in food intake regulation in fish brain

2020 ◽  
Vol 223 (17) ◽  
pp. jeb227330
Author(s):  
Cristina Velasco ◽  
Marta Conde-Sieira ◽  
Sara Comesaña ◽  
Mauro Chivite ◽  
Adrián Díaz-Rúa ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Free Fatty Acid ◽  
Food Intake ◽  
Mrna Abundance ◽  
Fish Brain ◽  
Protein Levels ◽  
Food Intake Regulation ◽  
Free Fatty Acid Receptors ◽  
Intake Regulation ◽  
Long Chain

ABSTRACTWe hypothesized that the free fatty acid receptors FFA1 and FFA4 might be involved in the anorectic response observed in fish after rising levels of long-chain fatty acids (LCFAs) such as oleate. In one experiment we demonstrated that intracerebroventricular (i.c.v.) treatment of rainbow trout with FFA1 and FFA4 agonists elicited an anorectic response 2, 6 and 24 h after treatment. In a second experiment, the same i.c.v. treatment resulted after 2 h in an enhancement in the mRNA abundance of anorexigenic neuropeptides pomca1 and cartpt and a decrease in the values of orexigenic peptides npy and agrp1. These changes occurred in parallel with those observed in the mRNA abundance and/or protein levels of the transcription factors Creb, Bsx and FoxO1, protein levels and phosphorylation status of Ampkα and Akt, and mRNA abundance of plcb1 and itrp3. Finally, we assessed in a third experiment the response of all these parameters after 2 h of i.c.v. treatment with oleate (the endogenous ligand of both free fatty acid receptors) alone or in the presence of FFA1 and FFA4 antagonists. Most effects of oleate disappeared in the presence of FFA1 and FFA4 antagonists. The evidence obtained supports the involvement of FFA1 and FFA4 in fatty acid sensing in fish brain, and thus involvement in food intake regulation through mechanisms not exactly comparable (differential response of neuropeptides and cellular signalling) to those known in mammals.

Role of paraventricular hypothalamic dopaminergic D1 receptors in food intake regulation of food-deprived rats

2018 ◽  
Vol 818 ◽  
pp. 43-49 ◽  
Author(s):  
Zahra. Mirmohammadsadeghi ◽  
Masoud. Shareghi Brojeni ◽  
Abbas. Haghparast ◽  
Afsaneh. Eliassi
Keyword(s):  
Food Intake ◽  
D1 Receptors ◽  
Food Intake Regulation ◽  
Intake Regulation

Food intake regulation in late pregnancy and early lactation

1999 ◽  
Vol 24 ◽  
pp. 37-54 ◽  
Author(s):  
K. L. Ingvartsen ◽  
N. C. Friggens ◽  
P. Faverdin
Keyword(s):  
Food Intake ◽  
Late Pregnancy ◽  
Reproductive Hormones ◽  
Early Lactation ◽  
Physical Constraints ◽  
Body Reserves ◽  
Food Intake Regulation ◽  
Periparturient Cows ◽  
Intake Regulation

AbstractThe dip in food intake, which starts in late pregnancy and continues into early lactation, has traditionally been interpreted as a depression in intake due to physical constraints. However, the rôle of physical constraints on intake has been overemphasized, particularly in early lactation. There is mounting evidence that the presence and mobilization of body reserves in early lactation play an important rôle in regulating intake at this time.Conceptually, the dip in intake in early lactation observed when cows have access to non-limiting foods can be accounted for by assuming that the cow has a desired level of body reserves. When the cow is not compromised, the changes with time in body reserves and the dip in intake represent the normal case and provide the basis against which to assess true depressions in intake which may occur when the cow is compromised by limiting nutrition or environment.The regulation of body reserves and intake in the periparturient cow is orchestrated through nervous and hormonal signals. Likely factors that are involved in intake regulation are reproductive hormones, neuropeptides, adrenergic signals, insulin and insulin resistance and leptin. Furthermore, oxidation of NEFA in the liver may result in feedback signals that reduce intake. The relative importance of these is discussed. A better understanding of the physiological signals involved in intake regulation and their interrelations with body weight regulation may provide important indicators of the degree of compromise that periparturient cows may experience.

Metabolic and mitogenic effects of IGF-II in rainbow trout (Oncorhynchus mykiss) myocytes in culture and the role of IGF-II in the PI3K/Akt and MAPK signalling pathways

2008 ◽  
Vol 157 (2) ◽  
pp. 116-124 ◽  
Author(s):  
Marta Codina ◽  
Daniel García de la serrana ◽  
Joan Sánchez-Gurmaches ◽  
Núria Montserrat ◽  
Oxana Chistyakova ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Signalling Pathways ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Mapk Signalling

scholarly journals Hypothalamic mechanisms linking fatty acid sensing and food intake regulation in rainbow trout

2017 ◽  
Vol 59 (4) ◽  
pp. 377-390 ◽  
Author(s):  
Cristina Velasco ◽  
Cristina Otero-Rodiño ◽  
Sara Comesaña ◽  
Jesús M Míguez ◽  
José L Soengas
Keyword(s):  
Fatty Acids ◽  
Transcription Factors ◽  
Fatty Acid ◽  
Rainbow Trout ◽  
Food Intake ◽  
Food Intake Regulation ◽  
Intake Regulation ◽  
First Time ◽  
Specific Inhibitors ◽  
Fatty Acid Sensing

We assessed in rainbow trout hypothalamus the effects of oleate and octanoate on levels and phosphorylation status of two transcription factors, FoxO1 and CREB, possibly involved in linking activation of fatty acid sensing with modulation of food intake through the expression of brain neuropeptides. Moreover, we assessed changes in the phosphorylation status of three proteins possibly involved in modulation of these transcription factors such as Akt, AMPK and mTOR. In a first experiment, we evaluated, in pools of hypothalamus incubated for 3 h and 6 h at 15°C in a modified Hanks’ medium containing 100 or 500 µM oleate or octanoate, the response of fatty acid sensing, neuropeptide expression and phosphorylation status of proteins of interest. The activation of fatty acid sensing and enhanced anorectic potential occurred in parallel with the activation of Akt and mTOR, and the inhibition of AMPK. The changes in these proteins would relate to a neuropeptide expression through changes in the phosphorylation status of transcription factors under their control, such as CREB and FoxO1, which displayed inhibitory (CREB) or activatory (FoxO1) responses when tissues were incubated with oleate or octanoate. In a second experiment, we incubated hypothalamus for 6 h with 500 µM oleate or octanoate alone or in the presence of specific inhibitors of Akt, AMPK, mTOR, CREB or FoxO1. The presence of inhibitors counteracted the effects of oleate or octanoate on the phosphorylation status of the proteins of interest. The results support, for the first time in fish, the involvement of these proteins in the regulation of food intake by fatty acids.

scholarly journals Bile acid composition regulates GPR119-dependent intestinal lipid sensing and food intake regulation in mice

Gut ◽  
2020 ◽  
Vol 69 (9) ◽  
pp. 1620-1628 ◽  
Author(s):  
Sei Higuchi ◽  
Tiara R Ahmad ◽  
Donovan A Argueta ◽  
Pedro A Perez ◽  
Chen Zhao ◽  
...  
Keyword(s):  
Gastric Emptying ◽  
Food Intake ◽  
Dietary Lipid ◽  
Chow Diet ◽  
Distal Intestine ◽  
Gut Hormone ◽  
Lipid Sensing ◽  
Food Intake Regulation ◽  
Intake Regulation

ObjectivesLipid mediators in the GI tract regulate satiation and satiety. Bile acids (BAs) regulate the absorption and metabolism of dietary lipid in the intestine, but their effects on lipid-regulated satiation and satiety are completely unknown. Investigating this is challenging because introducing excessive BAs or eliminating BAs strongly impacts GI functions. We used a mouse model (Cyp8b1–/– mice) with normal total BA levels, but alterations in the composition of the BA pool that impact multiple aspects of intestinal lipid metabolism. We tested two hypotheses: BAs affect food intake by (1) regulating production of the bioactive lipid oleoylethanolamide (OEA), which enhances satiety; or (2) regulating the quantity and localisation of hydrolysed fat in small intestine, which controls gastric emptying and satiation.DesignWe evaluated OEA levels, gastric emptying and food intake in wild-type and Cyp8b1–/– mice. We assessed the role of the fat receptor GPR119 in these effects using Gpr119–/– mice.ResultsCyp8b1–/– mice on a chow diet showed mild hypophagia. Jejunal OEA production was blunted in Cyp8b1–/– mice, thus these data do not support a role for this pathway in the hypophagia of Cyp8b1–/– mice. On the other hand, Cyp8b1 deficiency decreased gastric emptying, and this was dependent on dietary fat. GPR119 deficiency normalised the gastric emptying, gut hormone levels, food intake and body weight of Cyp8b1–/– mice.ConclusionBAs regulate gastric emptying and satiation by determining fat-dependent GPR119 activity in distal intestine.

Sign in / Sign up

Export Citation Format

Share Document