scholarly journals Strain specific differences in muscle Ca2+ transport and mitochondrial electron transport proteins between FVB/N and C57BL/6J mice

2020 ◽  
pp. jeb.238634
Author(s):  
Sushant Singh ◽  
Muthu Periasamy ◽  
Naresh C. Bal
Keyword(s):  
Electron Transport ◽  
Inbred Mice ◽  
Muscle Metabolism ◽  
Transport Proteins ◽  
Genetically Engineered ◽  
Mouse Strains ◽  
Future Studies ◽  
Genetically Engineered Mouse Models ◽  
Electron Transport Proteins

Genetically engineered mouse models have been used to determine the role of sarcolipin (SLN) in muscle. However, few studies had difficulty in detecting SLN in FBV/N mice and questioned its relevance to muscle metabolism. It is known that genetic alteration of proteins in different inbred mice strains produce dissimilar functional outcome. Therefore, here we compared the expression of SLN and key proteins involved in Ca2+-handling and mitochondrial metabolism between FVB/N and C57BL/6J mouse strains. Data suggests that, SLN expression is less abundant in the skeletal muscles of FVB/N mice compared to C57BL/6J strain. The expression of Ca2+-transporters in the mitochondrial membranes, were also lower in FVB/N compared to C57BL/6J. Similarly the electron transport proteins in the mitochondria were less abundant in FVB/N mice which may contribute to differences in energy metabolism. Future studies using different mouse strains should take these differences into account while interpreting their data.

The role of electrostatic interactions in the process of diffusional encounter and docking of electron transport proteins

2016 ◽  
Vol 468 (1) ◽  
pp. 183-186 ◽  
Author(s):  
I. B. Kovalenko ◽  
S. S. Khrushchev ◽  
V. A. Fedorov ◽  
G. Yu. Riznichenko ◽  
A. B. Rubin
Keyword(s):  
Electron Transport ◽  
Electrostatic Interactions ◽  
Transport Proteins ◽  
Electron Transport Proteins

The alternative oxidases: simple oxidoreductase proteins with complex functions

2013 ◽  
Vol 41 (5) ◽  
pp. 1305-1311 ◽  
Author(s):  
Luke Young ◽  
Tomoo Shiba ◽  
Shigeharu Harada ◽  
Kiyoshi Kita ◽  
Mary S. Albury ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Electron Transport ◽  
Alternative Oxidase ◽  
Structural Features ◽  
Transport Proteins ◽  
Integral Membrane Proteins ◽  
Complex Functions ◽  
Membrane Bound ◽  
Iron Carboxylate ◽  
Electron Transport Proteins

The alternative oxidases are membrane-bound monotopic terminal electron transport proteins found in all plants and in some agrochemically important fungi and parasites including Trypansoma brucei, which is the causative agent of trypanosomiasis. They are integral membrane proteins and reduce oxygen to water in a four electron process. The recent elucidation of the crystal structure of the trypanosomal alternative oxidase at 2.85 Å (1 Å=0.1 nm) has revealed salient structural features necessary for its function. In the present review we compare the primary and secondary ligation spheres of the alternative oxidases with other di-iron carboxylate proteins and propose a mechanism for the reduction of oxygen to water.

scholarly journals Role of Genetic Resistance in Invasive Pneumococcal Infection: Identification and Study of Susceptibility and Resistance in Inbred Mouse Strains

2001 ◽  
Vol 69 (1) ◽  
pp. 426-434 ◽  
Author(s):  
Neill A. Gingles ◽  
Janet E. Alexander ◽  
Aras Kadioglu ◽  
Peter W. Andrew ◽  
Alison Kerr ◽  
...  
Keyword(s):  
Inbred Mice ◽  
Inbred Mouse ◽  
Genetic Resistance ◽  
Pneumococcal Infection ◽  
Mouse Strains ◽  
Inflammatory Lesions ◽  
Invasive Pneumococcal Infection ◽  
Susceptibility And Resistance

ABSTRACT From a panel of nine inbred mice strains intranasally infected withStreptococcus pneumoniae type 2 strain, BALB/c mice were resistant and CBA/Ca and SJL mice were susceptible to infection. Further investigation revealed that BALB/c mice were able to prevent proliferation of pneumococci in the lungs and blood, whereas CBA/Ca mice showed no bacterial clearance. Rapidly increasing numbers of bacteria in the blood was a feature of CBA/Ca but not BALB/c mice. In the lungs, BALB/c mice recruited significantly more neutrophils than CBA/Ca mice at 12 and 24 h postinfection. Inflammatory lesions in BALB/c mice were visible much earlier than in CBA/Ca mice, and there was a greater cellular infiltration into the lung tissue of BALB/c mice at the earlier time points. Our data suggest that resistance or susceptibility to intranasal pneumococci may have an association with recruitment and/or function of neutrophils.

Neuropathology of Mowat–Wilson Syndrome

2020 ◽  
Vol 23 (4) ◽  
pp. 322-325
Author(s):  
Miriam R. Conces ◽  
Anna Hughes ◽  
Christopher R. Pierson
Keyword(s):  
Mouse Models ◽  
Hirschsprung Disease ◽  
Genetically Engineered ◽  
Facial Dysmorphism ◽  
Genetically Engineered Mouse Models ◽  
Brain Malformations ◽  
Neuroimaging Data ◽  
The Brain ◽  
Correlate Data

Mowat–Wilson syndrome (MWS) is a syndromic form of Hirschsprung disease that is characterized by variable degrees of intellectual disability, characteristic facial dysmorphism, and a diverse set of other congenital malformations due to haploinsufficiency of ZEB2. A variety of brain malformations have been described in neuroimaging studies of MWS patients, and the role of ZEB2 in the brain has been studied in a multitude of genetically engineered mouse models that are now available. However, a paucity of autopsy information limits our ability to correlate data from neuroimaging studies and animal models with actual MWS patient tissues. Here, we report the autopsy neuropathology of a 19-year-old male patient with MWS. Autopsy neuropathology findings correlated well with the reported MWS neuroimaging data and are in keeping with data from genetically engineered MWS mouse models. This autopsy enhances our understanding of ZEB2 function in human brain development and demonstrates the reliability of MWS murine models.

Differentiation of the Gastric Mucosa II. Role of gastrin in gastric epithelial cell proliferation and maturation

2006 ◽  
Vol 291 (5) ◽  
pp. G762-G765 ◽  
Author(s):  
Renu N. Jain ◽  
Linda C. Samuelson
Keyword(s):  
Genetically Engineered ◽  
Gastric Epithelial Cells ◽  
Balanced Growth ◽  
Cellular Targets ◽  
Genetically Engineered Mouse Models ◽  
Gastric Physiology ◽  
Ecl Cell ◽  
Acid Secreting ◽  
And Function

Gastrin is the principal hormonal inducer of gastric acid secretion. The cellular targets for gastrin in the stomach are the acid-secreting parietal cell and histamine-producing enterochromaffin-like (ECL) cell. Gastrin is also a growth factor, with hypergastrinemia resulting in increased proliferation of gastric progenitor cells and a thickened mucosa. This review presents insights into gastrin function revealed by genetically engineered mouse models, demonstrating a new role for gastrin in the maturation of parietal and ECL cells. Thus, gastrin regulates many aspects of gastric physiology, with tight regulation of gastrin levels required to maintain balanced growth and function of gastric epithelial cells.

Topography of the 27-and 31-kDa Electron Transport Proteins in the Onion Root Plasma Membrane

1995 ◽  
Vol 216 (3) ◽  
pp. 1054-1059 ◽  
Author(s):  
M.C. Cordoba ◽  
A. Serrano ◽  
F. Cordoba ◽  
J.A. Gonzalezreyes ◽  
P. Navas ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Electron Transport ◽  
Transport Proteins ◽  
Onion Root ◽  
Electron Transport Proteins ◽  
Root Plasma Membrane
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