scholarly journals Morphological colour adaptation during development in fish: involvement of growth hormone receptor 1

2020 ◽  
Vol 223 (24) ◽  
pp. jeb230375
Author(s):  
Tomás Horacio Delgadin ◽  
Diana Carolina Castañeda-Cortés ◽  
Clara Sacks ◽  
Andrés Breccia ◽  
Juan Ignacio Fernandino ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Hormone Receptor ◽  
Growth Hormone Receptor ◽  
Skin Pigmentation ◽  
Dual Function ◽  
Pituitary Hormone ◽  
Similar Response ◽  
Pituitary Cells ◽  
Larval Stages ◽  
Background Adaptation

ABSTRACTMorphological background adaptation is both an endocrine and a nervous response, involving changes in the amount of chromatophores and pigment concentration. However, whether this adaptation takes place at early developmental stages is largely unknown. Somatolactin (Sl) is a pituitary hormone present in fish, which has been associated to skin pigmentation. Moreover, growth hormone receptor type 1 (Ghr1) has been suggested to be the Sl receptor and was associated with background adaptation in adults. In this context, the aim of this work was to evaluate the ontogeny of morphological adaptation to background and the participation of ghr1 in this process. We found in larval stages of the cichlid Cichlasoma dimerus that the number of head melanophores and pituitary cells immunoreactive to Sl was increased in individuals reared with black backgrounds compared with that in fish grown in white tanks. In larval stages of the medaka Oryzias latipes, a similar response was observed, which was altered by ghr1 biallelic mutations using CRISPR/Cas9. Interestingly, melanophore and leucophore numbers were highly associated. Furthermore, we found that somatic growth was reduced in ghr1 biallelic mutant O. latipes, establishing the dual function of this growth hormone receptor. Taken together, these results show that morphological background adaptation is present at early stages during development and that is dependent upon ghr1 during this period.

scholarly journals Morphological colour adaptation during development: Involvement of Growth Hormone Receptor 1

2020 ◽  
Author(s):  
Tomás Horacio Delgadin ◽  
Diana Carolina Castañeda-Cortés ◽  
Clara Sacks ◽  
Andrés Breccia ◽  
Juan Ignacio Fernandino ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Hormone Receptor ◽  
Growth Hormone Receptor ◽  
Skin Pigmentation ◽  
Dual Function ◽  
Pituitary Hormone ◽  
Similar Response ◽  
Pituitary Cells ◽  
Larval Stages ◽  
Background Adaptation

AbstractMorphological background adaptation is both an endocrine and a nervous response, involving changes in the amount and shape of chromatophores. However, if this adaptation takes place at early developmental stages is largely unknown. Somatolactin (SL) is a pituitary hormone present in fish, which has been associated to skin pigmentation. Moreover, growth hormone receptor type 1 (ghr1) has been suggested to be the SL receptor and was associated to background adaptation in adults. In this context, the aim of this work was to evaluate the ontogeny of morphological adaptation to background and the participation of ghr1 in this process. We found in larval stages of Cichlasoma dimerus that the number of head melanophores and ir-SL pituitary cells were increased in individuals reared in black backgrounds compared to fish grown in white tanks. In medaka (Oryzias latipes) larval stages a similar response was observed that is altered by a ghr1 biallelic mutations using CRISPR/cas9. Interestingly, melanophore and leucophore numbers are highly associated. Furthermore, we found that somatic growth is reduced in ghr1 biallelic mutant medaka, establishing the dual function of this growth hormone receptor. Taken together, these results show that morphological background adaptation is present at early stages during development and that is dependent upon ghr1 unless during this period.

scholarly journals Preparation of a Growth Hormone Receptor/Prolactin Receptor Bispecific Antibody Antagonist Which Exhibited Anti-Cancer Activity

2020 ◽  
Vol 11 ◽  
Author(s):  
Xin Chen ◽  
Di Wu ◽  
Yan Zheng ◽  
Xingxing Liu ◽  
Jianmeng Wang
Keyword(s):  
Breast Cancer ◽  
Growth Hormone ◽  
Cancer Cell ◽  
Hormone Receptor ◽  
Intracellular Signaling ◽  
Growth Hormone Receptor ◽  
Prolactin Receptor ◽  
Dual Function ◽  
Internal Image ◽  
Idiotypic Antibody

Prolactin receptor (PRLR) and growth hormone receptor (GHR) are closely related to the occurrence and development of breast cancer, and breast cancer cell endogenously express GHR, PRLR and GHR-PRLR heterodimer. In this case, the combined use of PRLR or GHR inhibitors may produce better anti-breast cancer potential than PRLR or GHR inhibitors alone. In this case, it is necessary to develop the dual-function GHR/PRLR antagonists with anti-breast cancer potential. For this, we used hybridoma technology to generate an anti-idiotypic antibody (termed H53). We then used various techniques, including competitive ELISA, competitive receptor binding analysis, and indirect immunofluorescence assay to identify H53, and the results show that H53 behaves as a typical internal image anti-idiotypic antibody (Ab2β). Further experiments indicate that H53 is a dual-function inhibitor, which not only inhibited PRLR-mediated intracellular signaling, but also blocked GHR-mediated intracellular signaling in a dose-dependent manner. Furthermore, H53 could inhibit PRL/GH-driven cancer cell proliferation in vivo and in vitro. This study indicates that H53 exhibits potential biological activity against breast tumors, which implies that internal image anti-idiotypic antibodies may be a useful strategy for the development of PRLR/GHR dual-function antagonists for breast cancer therapy.

Laron syndrome – A case Report

JMS SKIMS ◽  
2017 ◽  
Vol 20 (2) ◽  
pp. 104-106
Author(s):  
Javaid Ahmad Bhat ◽  
Moomin Hussain Bhat ◽  
Hilal Bhat ◽  
Mona Sood ◽  
Shariq Rashid Masoodi
Keyword(s):  
Growth Hormone ◽  
Case Report ◽  
Hormone Receptor ◽  
Growth Hormone Receptor ◽  
Genetic Disorder ◽  
Female Child ◽  
Receptor Signaling ◽  
Laron Syndrome ◽  
Rare Genetic Disorder ◽  
Igf I

Background : Laron & colleagues (1966) reported a rare genetic disorder in Israliei Jewish sublings which was characterized by insensitivity to growth hormone due to abnormality in growth hormone receptor or post receptor signaling pathway.Case Report: We hereby report a case of a 5 year old female child who presented to us with features similar to Laron syndrome. The diagnosis was made & confirmed by various Lab. investigations like low IGF-I levels and managed accordingly. JMS 2017; 20 (2):104-106  

Three novel Growth Hormone Receptor (GHR) splicing mutations causing a spectrum of Growth Hormone Insensitivity

2019 ◽  
Author(s):  
Emily Cottrell ◽  
Avinaash Maharaj ◽  
Tasneem Ladha ◽  
Sumana Chatterjee ◽  
Anna Grandone ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Hormone Receptor ◽  
Growth Hormone Receptor ◽  
Splicing Mutations ◽  
Growth Hormone Insensitivity

Isolation of two types of cDNA encoding growth hormone receptor-like genes in the Japanese eel, Anguilla japonica

2002 ◽  
Vol 68 (sup1) ◽  
pp. 959-960 ◽  
Author(s):  
YUICHI OZAKI ◽  
HARUHISA FUKADA ◽  
YUKINORI KAZETO ◽  
SHINJI ADACHI ◽  
AKIHIKO HARA ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Hormone Receptor ◽  
Growth Hormone Receptor ◽  
Anguilla Japonica ◽  
Japanese Eel
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