scholarly journals Hypoxia acclimation alters reactive oxygen species homeostasis and oxidative status in estuarine killifish (Fundulus heteroclitus)

2020 ◽  
Vol 223 (13) ◽  
pp. jeb222877 ◽  
Author(s):  
Brittney G. Borowiec ◽  
Graham R. Scott
Keyword(s):  
Reactive Oxygen Species ◽  
Fundulus Heteroclitus ◽  
Reactive Oxygen ◽  
Oxidative Status ◽  
Oxygen Species ◽  
Hypoxia Acclimation

scholarly journals Thalassemic DNA-Containing Red Blood Cells Are under Oxidative Stress

Anemia ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Mutaz Dana ◽  
Eugenia Prus ◽  
Eitan Fibach
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Flow Cytometry ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Reactive Oxygen ◽  
Oxidative Status ◽  
Oxygen Species ◽  
Dna Breakage ◽  
Erythroid Precursors

We studied the nature of enucleated RBCs containing DNA remnants, Howell-Jolly (HJ) RBCs and reticulocytes (retics), that are characteristically present in the circulation of thalassemic patients, especially after splenectomy. Using flow cytometry methodology, we measured oxidative status parameters of these cells in patients withβ-thalassemia. In each patient studied, these cells had higher content of reactive oxygen species and exposed phosphatidylserine compared with their DNA-free counterparts. These results suggest that oxidative stress in thalassemic developing erythroid precursors might, through DNA-breakage, generate HJ-retics and HJ-RBCs and that oxidative stress-induced externalization of phosphatidylserine is involved in the removal of these cells from the circulation by the spleen, a mechanism similar to that of the removal of senescent RBCs.

A rapid method to assess reactive oxygen species in yeast using H2DCF-DA

2015 ◽  
Vol 7 (20) ◽  
pp. 8572-8575 ◽  
Author(s):  
Joel James ◽  
Nikita Fiji ◽  
Debasish Roy ◽  
Daniel Andrew MG ◽  
Mohamed Sham Shihabudeen ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Rapid Method ◽  
Reactive Oxygen ◽  
Oxidative Status ◽  
Oxygen Species

A rapid method to extract DCF from yeast to measure its oxidative status by using a fluorescence spectrophotometer.

scholarly journals Spectrophotometric assays for evaluation of Reactive Oxygen Species (ROS) in serum: general concepts and applications in dogs and humans

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Camila Peres Rubio ◽  
José Joaquin Cerón
Keyword(s):  
Reactive Oxygen Species ◽  
Oxidative Damage ◽  
One Health ◽  
Reactive Oxygen ◽  
Oxidative Status ◽  
Biological Macromolecules ◽  
Oxygen Species ◽  
Limited Information ◽  
Practical Applications ◽  
Spectrophotometric Methods

AbstractReactive oxygen species (ROS) are reactive compounds derived from oxygen. In biological systems, an excessive amount of ROS can cause oxidative damage to biological macromolecules being involved in different diseases. Several assays have been developed in the last 30 years for ROS evaluation. The objective of this article will be to provide an update about the spectrophotometric methods currently used in the assessment of ROS in serum. The chemical basis of four different techniques will be reviewed, and examples of their possible applications will be provided. A particular emphasis about the practical applications of these assays in the dog will be made, but selected information about their use in humans will also be presented for comparative purposes, following a One-Health approach. The information about the spectrophotometric assays presented in this paper should be interpreted with caution once limited information about them is available yet, and further studies should be performed to clarify what they measure and their clinical application. Ideally, when applied to evaluate a sample’s oxidative status, they should be incorporated in a panel of analytes where other oxidants, antioxidants, and biomarkers of inflammation were also included.

scholarly journals Modulation of Adipogenesis and Oxidative Status by Quercetin and Ochratoxin A: Positive or Negative Impact on Rat Adipocyte Metabolism?

Molecules ◽  
2019 ◽  
Vol 24 (20) ◽  
pp. 3726 ◽  
Author(s):  
Viktoria Dobrocsyova ◽  
Katarina Krskova ◽  
Marcela Capcarova ◽  
Stefan Zorad
Keyword(s):  
Reactive Oxygen Species ◽  
Adipose Tissue ◽  
Glucose Uptake ◽  
Ochratoxin A ◽  
Negative Impact ◽  
Adipogenic Differentiation ◽  
Reactive Oxygen ◽  
Oxidative Status ◽  
Ros Production ◽  
Oxygen Species

(1) Background: Impaired adipose tissue function leads to the development of metabolic disorders. Reactive oxygen species play a key role in the regulation of adipogenesis and insulin-stimulated glucose uptake by adipocytes. Quercetin (QCT) regulates adipogenesis by affecting the redox state of preadipocytes. Ochratoxin A (OTA) is one of the most prevalent mycotoxins contaminating food. It has cytotoxic, genotoxic, pro-inflammatory, and anti-adipogenic effects. Antioxidants are believed to protect cells from the cytotoxicity and genotoxicity induced by OTA. The aim of this study was to investigate the effect of QCT and OTA application on preadipocyte differentiation, oxidative status, and adipocyte metabolism. (2) Methods: Primary rat preadipocytes were isolated from subcutaneous adipose tissue of Wistar rats. Gene expressions were determined by qPCR. Cell viability, reactive oxygen species (ROS) production, glucose uptake, and lipid accumulation were determined using commercially available kits. (3) Results: A dose-dependent inhibitory effect of QCT on adipogenic differentiation was observed, which was accompanied by a decrease in ROS production. Reduced ROS formation is closely related to impaired glucose uptake by adipocytes. (4) Conclusions: The results of this study indicate a key role of ROS in regulating adipogenesis and metabolic pathways, which is affected by the application of QCT and/or OTA.

scholarly journals Cocoa and Cocoa Fibre Intake Modulate Reactive Oxygen Species and Immunoglobulin Production in Rats Submitted to Acute Running Exercise

Proceedings ◽  
2020 ◽  
Vol 61 (1) ◽  
pp. 30
Author(s):  
Patricia Ruiz-Iglesias ◽  
Raquel Gómez-Bris ◽  
Malén Massot-Cladera ◽  
María J. Rodríguez-Lagunas ◽  
Francisco J. Pérez-Cano ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Acute Exercise ◽  
Reactive Oxygen ◽  
Oxidative Status ◽  
Ros Production ◽  
Oxygen Species ◽  
Single Session ◽  
Immunoglobulin Production ◽  
Immune Functioning ◽  
Running Exercise

Acute high-intensity exercise can impair the immune system, and lead to oxidative stress. Cocoa intake might help in protecting against oxidative damage and impaired immune functioning. The aim of this study was to establish the effect of cocoa and cocoa fibre on the oxidative status and the immunoglobulin (Ig) production of rats following a bout of acute exercise on a treadmill. The production of reactive oxygen species (ROS) by macrophages and the concentration of serum and mucosal Ig was assessed 16 h after the running session. Exercise increased ROS production and decreased the serum IgG concentration and the salivary gland IgM content. A cocoa fibre-enriched diet prevented the increased ROS production and the reduction in salivary IgM induced by exercise, although it decreased the IgA content in serum and the salivary glands. Overall, cocoa, by means of its fibre content, can partially prevent the alterations in ROS and Ig production induced by a single session of intensive running exercise.

Mitochondria as a source of reactive oxygen species

2009 ◽  
pp. c3 ◽  
Author(s):  
Helena M. Cochemé ◽  
Michael P. Murphy
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Oxygen Species

Clinical aspects of reactive oxygen and nitrogen species

2004 ◽  
Vol 71 ◽  
pp. 121-133 ◽  
Author(s):  
Ascan Warnholtz ◽  
Maria Wendt ◽  
Michael August ◽  
Thomas Münzel
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Risk Factor ◽  
Endothelial Dysfunction ◽  
Vascular Tissue ◽  
Rapid Development ◽  
Reactive Oxygen ◽  
Clinical Aspects ◽  
No Production ◽  
Oxygen Species

Endothelial dysfunction in the setting of cardiovascular risk factors, such as hypercholesterolaemia, hypertension, diabetes mellitus and chronic smoking, as well as in the setting of heart failure, has been shown to be at least partly dependent on the production of reactive oxygen species in endothelial and/or smooth muscle cells and the adventitia, and the subsequent decrease in vascular bioavailability of NO. Superoxide-producing enzymes involved in increased oxidative stress within vascular tissue include NAD(P)H-oxidase, xanthine oxidase and endothelial nitric oxide synthase in an uncoupled state. Recent studies indicate that endothelial dysfunction of peripheral and coronary resistance and conductance vessels represents a strong and independent risk factor for future cardiovascular events. Ways to reduce endothelial dysfunction include risk-factor modification and treatment with substances that have been shown to reduce oxidative stress and, simultaneously, to stimulate endothelial NO production, such as inhibitors of angiotensin-converting enzyme or the statins. In contrast, in conditions where increased production of reactive oxygen species, such as superoxide, in vascular tissue is established, treatment with NO, e.g. via administration of nitroglycerin, results in a rapid development of endothelial dysfunction, which may worsen the prognosis in patients with established coronary artery disease.

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