On the Penetration of Insecticides Through the Insect Cuticle
1. Using Melophagus ovinus, the sheep ked, as test insect, it was found that certain organic solvents of diphenylamine, such as the cresols, benzyl alcohol and 4-methyl-cyclohexanol, greatly increase the rate of action of this insecticide. Others, such as carbitol and methyl benzoate, gave little or no improvement in the time of kill. The degree to which a solvent induces rapid penetration of an insecticide is referred to as its ‘carrier efficiency’. 2. The influence of the physical properties of the solvents on carrier efficiency was investigated. It was found that a high carrier efficiency could be correlated with a high rate of penetration through beeswax, a high partition coefficient of the solvent between beeswax and water and a high solubility of insecticide in a solution of the solvent in water. The volatility of the solvent and the solubility of insecticide in solvent were also contributory factors. 3. Mixtures of two solvents, each showing no carrier efficiency but together possessing all the essential physical properties, were tested and showed a carrier efficiency considerably higher than that of either constituent. This is taken as supporting evidence that carrier efficiency depends on certain physical properties of a solvent. 4. Using a range of solvents shown to exhibit various degrees of carrier efficiency with diphenyl-amine, comparable results were obtained with dixanthogen, ω-nitrostyrene dibromide and rotenone and showed that the synergy could be extended to other insecticides. 5. It is suggested that certain solvents increase the rate of penetration of contact insecticides through the insect cuticle: (a) By transporting the insecticide through the lipoid elements of the epicuticle to the interface between this layer and the water permeating the exocuticle. (b) By concentrating the insecticide at the interface between the epicuticle and the exocuticle, as the solvent passes into the exocuticle, and thus increasing the diffusion gradient of the insecticide across that interface. (c) By increasing the solubility of the insecticide in the water permeating exo- and endo-cuticles and thus, by raising its partition coefficient between solvent in the epicuticle and water in the exocuticle, further increasing its rate of diffusion, not only across this interface, but also through exo- and endo-cuticles to the hypodermis.