Active and electrogenic absorption of Na + and Cl- across posterior gills of Eriocheir sinensis: influence of short-term osmotic variations

1996 ◽  
Vol 199 (4) ◽  
pp. 901-910 ◽  
Author(s):  
H Onken
Keyword(s):  
Electromotive Force ◽  
Marked Decrease ◽  
Short Circuit ◽  
Short Circuit Current ◽  
Eriocheir Sinensis ◽  
Induced Current ◽  
Na Current ◽  
Short Term ◽  
External Barrier ◽  
Short Circuit Currents

Split lamellae of posterior gills of Chinese crabs (Eriocheir sinensis) acclimated to fresh water were mounted in a modified Ussing-type chamber, and the transepithelial short-circuit current and conductance were measured. The epithelium shows independent active and electrogenic absorption mechanisms for Na+ and Cl- that can be measured as positive and negative short-circuit currents, respectively, in the absence of the counter ion. Increasing the osmolarity of the haemolymph-side saline by addition of sucrose resulted in a marked decrease in active uptake of both Na+ and Cl-. In contrast, increasing the internal osmolarity by addition of urea or moderately decreasing the haemolymph-side osmolarity resulted in a marked increase in Na+ as well as Cl- transport. Circuit analysis revealed that Na+ current changes are mostly due to alterations in the apical amiloride-sensitive Na+ conductance, while Cl- current changes are caused not only by alterations in the transcellular conductance but also by changes in the electromotive force for Cl- absorption. Osmotic perturbations in the external bath induced current changes in the same directions, but the magnitudes of the effects were smaller than those after internal osmotic variations, indicating that the external barrier has a lower water permeability than the internal barrier. Short-term osmotic perturbations did not significantly affect the leak conductance, which is not associated with active transport and which may mostly reflect the paracellular conductance.

Active NaCl absorption across split lamellae of posterior gills of the chinese crab Eriocheir sinensis: stimulation by eyestalk extract

2000 ◽  
Vol 203 (8) ◽  
pp. 1373-1381 ◽  
Author(s):  
H. Onken ◽  
A. Schobel ◽  
J. Kraft ◽  
M. Putzenlechner
Keyword(s):  
Electromotive Force ◽  
Single Channel ◽  
Short Circuit ◽  
Noise Analysis ◽  
Eriocheir Sinensis ◽  
Dialysis Tubing ◽  
Channel Current ◽  
Dose Dependent ◽  
Cl Conductance ◽  
Stimulation Of

Split lamellae of the posterior gills of freshwater-adapted Chinese crabs (Eriocheir sinensis) were mounted in a modified Ussing-type chamber, and active and electrogenic absorption of Na(+) and Cl(−) were measured as positive (I(Na)) or negative (I(Cl)) short-circuit currents. Haemolymph-side addition of eyestalk extract stimulated I(Cl) by increasing both the transcellular Cl(−) conductance and the electromotive force for Cl(−) absorption. The effect was dose-dependent. Boiling the eyestalk extract did not change its effectiveness. The stimulating factor passed through dialysis tubing, indicating that it has a molecular mass of less than 2 kDa. R(p)cAMPS, a blocker of protein kinase A, reduced the stimulated I(Cl). Eyestalk extract stimulated I(Na) by increasing the transcellular Na(+) conductance at constant electromotive force. Amiloride-induced current-noise analysis revealed that stimulation of I(Na) was accompanied by an increase in the apparent number of open apical Na(+) channels at a slightly reduced single-channel current. In addition to the electrophysiological experiments, whole gills were perfused in the presence and in the absence of putative transport stimulators, and the specific activities of the V-ATPase and the Na(+)/K(+)-ATPase were measured. Eyestalk extract, theophylline or dibutyryl-cyclic AMP stimulated the activity of the V-ATPase, whereas the activity of the Na(+)/K(+)-ATPase was unaffected. The simultaneous presence of R(p)cAMPS prevented the stimulation of V-ATPase by eyestalk extract or theophylline.

Na and Cl Transport across the Isolated Anterior Intestine of the Plaice Pleuronectes Platessa

1981 ◽  
Vol 90 (1) ◽  
pp. 123-142
Author(s):  
M. M. P. RAMOS ◽  
J. C. ELLORY
Keyword(s):  
Chloride Transport ◽  
Short Circuit ◽  
Short Circuit Current ◽  
Negative Potential ◽  
Loop Diuretics ◽  
Significant Part ◽  
Cl Transport ◽  
Anterior Intestine ◽  
Short Circuit Currents ◽  
Na Uptake

1. The tissue was found to have a serosa negative potential, and short-circuit currents equivalent to the net Cl transport. 2. A significant part of the Cl uptake was Na dependent and a similar fraction of the Na uptake was Cl dependent. 3. Short-circuit current and uptake of both ions were inhibited by loop diuretics and analogues. 4. I80 and P.D. were abolished by ouabain. 5. The observations are consistent with the idea of a coupled NaCl entry into the cell, using the energy inherent in the Na gradient; Na being pumped out of the cells by the Na pump and followed electrically by Cl−. Net chloride transport and the serosa negative potential would be a consequence of the permselective properties of the junctions allowing Na but not Cl to recycle back to the mucosal solution.

CYCLIC AMP STIMULATION OF ELECTROGENIC UPTAKE OF Na+ AND Cl- ACROSS THE GILL EPITHELIUM OF THE CHINESE CRAB ERIOCHEIR SINENSIS

1994 ◽  
Vol 188 (1) ◽  
pp. 159-174 ◽  
Author(s):  
S Riestenpatt ◽  
W Zeiske ◽  
H Onken
Keyword(s):  
Cyclic Amp ◽  
Electromotive Force ◽  
Single Channel ◽  
Short Circuit ◽  
Ussing Chamber ◽  
Eriocheir Sinensis ◽  
Na Channel ◽  
Before And After ◽  
Gill Lamellae ◽  
Stimulation Of

Split gill lamellae (epithelium plus cuticle) of hyperregulating Chinese crabs acclimated to fresh water were mounted in a modified Ussing chamber. Active and electrogenic absorption of sodium and chloride were measured as positive amiloride-sensitive and negative Cl--dependent short-circuit currents (INa, ICl), respectively. Both currents were characterized before and after treatment of the tissue with theophylline or dibutyryl cyclic AMP. Both drugs increased INa and ICl. A simple circuit analysis showed that INa stimulation reflected a marked increase in the transcellular Na+ conductance, whereas the respective electromotive force was unchanged. The Michaelis constant (KNa) for Na+ current saturation was decreased after INa stimulation, indicating an increased affinity of the transport mechanism for its substrate. Consequently, the affinity for the Na+ channel blocker amiloride decreased as expected for a competitive interaction between substrate and inhibitor. Analysis of the amiloride-induced current-noise revealed a marked increase in the number of apical Na+ channels after INa stimulation with theophylline, whereas there was little change in the single-channel current. Stimulation of Cl- absorption was accompanied by a substantial increase in both transcellular conductance and electromotive force, indicating an activation of the apical H+ pump that provides the driving force for active Cl- uptake via apical Cl-/HCO3- exchange and basolateral Cl- channels.

Analytical and Experimental Studies on Induced Current Switching by a Fault-Proof Gas Insulated Earthing Switch

2010 ◽  
Vol 11 (5) ◽  
Author(s):  
Mandava Mohana Rao ◽  
Moutusi Paul ◽  
H.S. Jain
Keyword(s):  
Short Circuit ◽  
Experimental Studies ◽  
Short Circuit Current ◽  
Design Parameters ◽  
Induced Current ◽  
Current Switching ◽  
Test Circuit ◽  
Reliable Performance ◽  
Gas Insulated Substation ◽  
The Impact

Fault-proof earthing switches are one of the important modules of a gas insulated substation, as it enables make at 100 percent short circuit current, which is functionally different from maintenance earthing switches. The fault-proof earthing switch shall be designed to make and break electro-magnetically and electro-statically induced currents as per IEC-62271-102. The paper discusses the impact of “test circuit configurations and voltage” on test parameters for gas insulated fault-proof earthing switch utilizing simulation with PSCAD software. Authors record the development of a 145 kV gas insulated fault proof earthing switch by considering novel design features like minimum arcing/pre-arcing time, effective current transfer from arcing contact to ground terminal, etc. The development has been evaluated successfully for electro-magnetically and electro-statically induced current duties as per IEC. Finally, design parameters to be considered for ensuring reliable performance during induced current switching from fault-proof earthing switches are also discussed.

scholarly journals Frequency and time fault diagnosis methods of power transformers

2018 ◽  
Vol 18 (4) ◽  
pp. 162-167 ◽  
Author(s):  
Miroslav Gutten ◽  
Daniel Korenciak ◽  
Matej Kucera ◽  
Richard Janura ◽  
Adam Glowacz ◽  
...  
Keyword(s):  
Fault Diagnosis ◽  
High Voltage ◽  
Impact Test ◽  
Power Transformer ◽  
Short Circuit ◽  
Small Deformation ◽  
Short Circuit Current ◽  
Diagnostic Methods ◽  
Response Analysis ◽  
Short Circuit Currents

Abstract The authors describe experimental and theoretical analyses of faults of power transformer winding. Faults were caused by mechanical effect of short-circuit currents. Measurements of transformer were carried out in high-voltage laboratory. Frequency and time diagnostic methods (method SFRA - Sweep Frequency Response Analysis, impact test) were used for the analyses. Coils of transformer windings were diagnosed by means of the SFRA method and the time impact test. The analyzed methods had a significant sensitivity to a relatively small deformation of coil. In the analysis a new technique for analyzing the effects of short-circuit currents is introduced. This technique is developed for high-voltage transformers (different types of power). The proposed analyses show that it is necessary to analyze the value of short-circuit current. Short-circuit current represents a danger for the operation of the power transformer. The proposed approach can be used for other types of transformers. Moreover, the presented techniques have a potential application for fault diagnosis of electrical equipment such as: transformers and electrical machines.

Cholinergic modulation of apical Na+ channels in turtle colon: analysis of CDPC-induced fluctuations

1990 ◽  
Vol 259 (4) ◽  
pp. C668-C674 ◽  
Author(s):  
D. J. Wilkinson ◽  
D. C. Dawson
Keyword(s):  
Single Channel ◽  
Short Circuit ◽  
Basolateral Membrane ◽  
Short Circuit Current ◽  
Rate Coefficients ◽  
Corner Frequency ◽  
Na Channel ◽  
Fluctuation Analysis ◽  
Na Channels ◽  
Na Current

Current fluctuation analysis was used to investigate the properties of apical Na+ channels during muscarinic inhibition of active Na+ absorption. A reversible Na+ channel blocker, 6-chloro-3,5-diaminopyrazine-2-carboxamide (CDPC), was used to induce fluctuations in the short-circuit current (I(sc)). Power density spectra of the CDPC-induced fluctuations exhibited a clearly discernible Lorentzian component, characterized by a corner frequency that was linearly related to CDPC concentration between 20 and 100 microM. The on (k'on) and off (k(off)) rate coefficients for the CDPC blocking reaction were k'on = 11.1 +/- 0.8 rad.s-1.microM-1 and k(off) = 744 +/- 53 rad/s, and the microscopic inhibition constant was 67 microM (n = 11). CDPC blocking kinetics were not significantly different after inhibition of Isc by 5 microM serosal carbachol. Single-channel Na+ current (iNa) and the density of open and blocked Na+ channels (N(ob)) were estimated from the fluctuations induced by 40 microM CDPC. Under control conditions, iNa was 0.43 +/- 0.05 pA and N(ob) was 251 +/- 42 X 10(6)/cm2 (n = 10). After exposure to serosal carbachol (2-10 microM) for 60 min, Na+ current and N(ob) were reduced by approximately 50%, but iNa was not changed significantly. These results indicate that muscarinic inhibition of electrogenic Na+ absorption was associated with a reduction in the number of open Na+ channels in the apical membrane. They also suggest that this downregulation of transport involved a coordinated decrease in both apical and basolateral membrane conductances.

Suppression of Short-Circuit Current with Embedded Source Resistance in SiC-MOSFET

2018 ◽  
Vol 924 ◽  
pp. 727-730 ◽  
Author(s):  
Hideyuki Hatta ◽  
Takaaki Tominaga ◽  
Shiro Hino ◽  
Naruhisa Miura ◽  
Shingo Tomohisa ◽  
...  
Keyword(s):  
Short Circuit ◽  
Short Circuit Current ◽  
Trade Off ◽  
Channel Resistance ◽  
Source Resistance ◽  
Short Circuit Currents

This work reports an SiC-MOSFET which replaces a part of the channel resistance with an additional embedded resistance, called a source resistance (Rs). MOSFETs with Rs have higher resistance during short circuit compared with MOSFETs without Rs and suppress short-circuit currents. An improvement of the trade-off relationship between short-circuit capability and on-resistance was obtained with MOSFETs including embedded Rs.

scholarly journals A V-ATPase drives active, electrogenic and Na+-independent Cl- absorption across the gills of Eriocheir sinensis

1995 ◽  
Vol 198 (3) ◽  
pp. 767-774 ◽  
Author(s):  
H Onken ◽  
M Putzenlechner
Keyword(s):  
Atpase Activity ◽  
Specific Activity ◽  
Short Circuit ◽  
Specific Inhibitor ◽  
Membrane Vesicles ◽  
Short Circuit Current ◽  
Eriocheir Sinensis ◽  
Similar Distribution ◽  
Fluorescence Studies ◽  
F1fo Atpase

Using biochemical and electrophysiological techniques, we have examined the proposal that an H+-ATPase is involved in Cl- uptake across the gills of the Chinese crab Eriocheir sinensis. Bafilomycin A1 (1 µmol l-1), a specific inhibitor of V-ATPases, was used to investigate the importance of this H+-translocating enzyme in Cl- transport across the gill. In homogenates of ion-transporting posterior gills, we found the activity of a bafilomycin-sensitive V-ATPase to be markedly higher than in the anterior gills, which are not involved in ion transport. A similar distribution was found for the Na+/K+- and the mitochondrial F1Fo-ATPase. After differential and density centrifugation, the specific activity of the V-ATPase was enriched by a factor of 5. Neither Na+/K+- and F1Fo-ATPase activities nor acid phosphatase activity copurified with the bafilomycin-sensitive ATPase activity, indicating that at least the major portion of V-ATPase activity is not of basolateral, mitochondrial or lysosomal origin. In fluorescence studies, using Acridine Orange or Oxonol V as dyes, membrane vesicles displayed ATP-dependent proton transport and membrane potential generation, which were markedly reduced in the presence of bafilomycin. In addition to these biochemical studies, we mounted split lamellae of posterior gills in an Ussing-type chamber and measured the negative short-circuit current (Isc), which was shown to reflect active, electrogenic, Na+-independent and ouabain-insensitive Cl- absorption. After the addition of 1 µmol l-1 bafilomycin to the external bath, this Isc was reduced to about 50­60 % of its original value. Concomitantly, the conductance of the preparation decreased by about 13 %. From these results, we conclude that an apical V-ATPase drives electrogenic Cl- uptake across the posterior gills of the Chinese crab.

scholarly journals Nerve Stimulation and Electrical Properties of Frog Skin

1969 ◽  
Vol 53 (4) ◽  
pp. 427-449 ◽  
Author(s):  
Barry D. Lindley
Keyword(s):  
Electrical Properties ◽  
Electromotive Force ◽  
Frog Skin ◽  
Short Circuit ◽  
Ionic Composition ◽  
Short Circuit Current ◽  
Exocrine Glands ◽  
Skin Glands ◽  
Chord Conductance ◽  
Frog Skin Glands

The suitability of frog skin glands as a model for the study of secretory mechanisms in exocrine glands was explored. Periodic voltage clamp was used to determine continually the short-circuit current, chord conductance, and electromotive force of frog skin during neural and pharmacological activation of the skin glands. Both the chord conductance and the short-circuit current increased with glandular activation; the temporal dissociation of these increases suggests that there are at least two separate components to the secretory response. The sensitivity of the secretory electrical changes to changes in the ionic composition of the bathing solutions supports the notion of electrogenic chloride active transport as being basic to the activity of the exocrine glands.

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