scholarly journals Latching of the click beetle (Coleoptera: Elateridae) thoracic hinge enabled by the morphology and mechanics of conformal structures

2019 ◽  
Vol 222 (12) ◽  
pp. jeb196683 ◽  
Author(s):  
Ophelia Bolmin ◽  
Lihua Wei ◽  
Alexander M. Hazel ◽  
Alison C. Dunn ◽  
Aimy Wissa ◽  
...  
Keyword(s):  
Click Beetle ◽  
Conformal Structures

scholarly journals Estimation of abundance and dispersal distance of the sugarcane click beetle Melanotus sakishimensis Ohira (Coleoptera: Elateridae) on Kurima Island, Okinawa, by mark-recapture experiments

2008 ◽  
Vol 43 (3) ◽  
pp. 409-419 ◽  
Author(s):  
Norio Arakaki ◽  
Atsushi Nagayama ◽  
Aya Kobayashi ◽  
Kazuhiko Tarora ◽  
Mitsunobu Kishita ◽  
...  
Keyword(s):  
Dispersal Distance ◽  
Mark Recapture ◽  
Click Beetle

scholarly journals Dimension rigidity in conformal structures

2017 ◽  
Vol 308 ◽  
pp. 1127-1186 ◽  
Author(s):  
Tushar Das ◽  
David Simmons ◽  
Mariusz Urbański
Keyword(s):  
Conformal Structures

scholarly journals A novel thermostable beetle luciferase based cytotoxicity assay

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sunju Choi ◽  
Hittu Matta ◽  
Ramakrishnan Gopalakrishnan ◽  
Venkatesh Natarajan ◽  
Songjie Gong ◽  
...  
Keyword(s):  
Membrane Integrity ◽  
Cytotoxicity Assay ◽  
Luciferase Assay ◽  
Target Cells ◽  
Over Expression ◽  
Click Beetle ◽  
In Vivo Bioluminescence Imaging ◽  
Cost Efficient ◽  
Dying Cells

AbstractCytotoxicity assays are essential for the testing and development of novel immunotherapies for the treatment of cancer. We recently described a novel cytotoxicity assay, termed the Matador assay, which was based on marine luciferases and their engineered derivatives. In this study, we describe the development of a new cytotoxicity assay termed ‘Matador-Glo assay’ which takes advantage of a thermostable variant of Click Beetle Luciferase (Luc146-1H2). Matador-Glo assay utilizes Luc146-1H2 and D-luciferin as the luciferase-substrate pair for luminescence detection. The assay involves ectopic over-expression of Luc146-1H2 in the cytosol of target cells of interest. Upon damage to the membrane integrity, the Luc146-1H2 is either released from the dead and dying cells or its activity is preferentially measured in dead and dying cells. We demonstrate that this assay is simple, fast, specific, sensitive, cost-efficient, and not labor-intensive. We further demonstrate that the Matador-Glo assay can be combined with the marine luciferase-based Matador assay to develop a dual luciferase assay for cell death detection. Finally, we demonstrate that the Luc146-1H2 expressing target cells can also be used for in vivo bioluminescence imaging applications.

scholarly journals Comparison of different pheromone traps for monitoring of click beetle Agriotes ustulatus (Coleoptera Elateridae)

2017 ◽  
pp. 155-159
Author(s):  
Tímea Szalárdi ◽  
Katalin Szentannai ◽  
Antal Nagy
Keyword(s):  
Sex Pheromone ◽  
Pheromone Traps ◽  
Preliminary Results ◽  
Click Beetle ◽  
The Way

The effectiveness of two trap types (YATLORf and VARb3 with CSALOMON® sex pheromone bait) for monitoring click beetle Agriotes ustu latus was compared near Karcag (East Hungary) in 2016. Additionally effectiveness of YATLORf traps placed on ground and placed on a 25 cm high mound was also compared. Contrary to our expectations traps caught very few individuals thus our study could provide only preliminary results. Between traps and methods there were not significant differences but YATLORf traps placed on mounds caught more individuals than others. The swarming imagos of A. ustulatus were also detected two weeks earlier in 2016 than as usual. To prove the effect of the way of usage on the effectiveness of YATLORf traps new studies should be made.

scholarly journals Taxonomic notes, a new species, and a key to Indian species of the click beetle genus Cryptalaus Ȏhira, 1967 (Coleoptera: Elateridae: Agrypninae)

2021 ◽  
Vol 13 (13) ◽  
pp. 19985-19999
Author(s):  
Harshad Parekar ◽  
Amol Patwardhan
Keyword(s):  
New Species ◽  
Morphological Characters ◽  
Indian Species ◽  
Male And Female ◽  
Median Ridge ◽  
Click Beetle ◽  
A New Species

Cryptalaus alveolatus sp. nov. is described and illustrated from India. The species has unique morphological characters: conspicuous longitudinal median ridge on pronotum, elytral interstria II more depressed than interstriae I and III. The Indian fauna of the genus Cryptalaus Ȏhira, 1967 is updated and represented by seven species: C. alveolatus sp. nov., C. assamensis (Schwarz, 1902) comb. nov., C. eryx (Candèze, 1874), C. lynceus (Candèze, 1874), C. nodulosus (Waterhouse, 1877) comb. nov., C. sculptus (Westwood, 1848) and C. sordidus (Westwood, 1848). Genitalia and terminalia of male and female C. sordidus are also illustrated. A key to the Indian species of Cryptalaus is given.

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