scholarly journals Partial purification, tissue distribution and modulatory activity of a crustacean cholecystokinin-like peptide.

1994 ◽  
Vol 187 (1) ◽  
pp. 181-200 ◽  
Author(s):  
G G Turrigiano ◽  
A Van Wormhoudt ◽  
L Ogden ◽  
A I Selverston
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Spiny Lobster ◽  
Reversed Phase ◽  
Partial Purification ◽  
Dependent Manner ◽  
Gastric Mill ◽  
Panulirus Interruptus ◽  
Dose Dependent ◽  
Cck Antagonist

Reversed-phase chromatography was used to separate several forms of cholecystokinin-like peptides (CCKLP) from the pericardial organs (PCOs) of the spiny lobster Panulirus interruptus. Fast protein liquid chromatography of PCOs, stomatogastric ganglia (STGs) and eyestalks revealed five peaks of CCKLP (peaks A-E) that were common to all three tissues, as well as two additional peaks (peaks F and G) in the STG. Peaks A-E were present in the hemolymph of fed, but not starved, lobsters. The bioactivity of peaks A-E was tested on the gastric mill rhythm of the isolated STG. Only peak E elicited activity. The effects of peak E included activating the gastric mill rhythm in quiescent preparations and strengthening existing rhythms in a dose-dependent manner. Further purification of peak E by high performance liquid chromatography resolved this peak into two immunoreactive peaks, one of which retained its bioactivity. The effects of peak E were blocked by the CCK antagonist proglumide. These results are consistent with a role for peak E in the feeding-induced activation of the gastric mill.

scholarly journals Identification and antioxidant activity of carotenoids from superfine powder of Rhodobacter sphaeroides

2017 ◽  
pp. 833 ◽  
Author(s):  
Zuming Li ◽  
Lina Kong ◽  
Bodi Hui ◽  
Xiaoya Shang ◽  
Liping Gao ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Rhodobacter Sphaeroides ◽  
Ultrasonic Treatment ◽  
High Performance ◽  
Reversed Phase ◽  
Natural Antioxidant ◽  
Dependent Manner ◽  
Rp Hplc

The interest in carotenoids from the natural-antioxidant point of view has recently risen sharply because of their substantial health benefits. Here, we report the identification and antioxidant activity of carotenoids extracted by superfine grinding from Rhodobacter sphaeroides 3757 which is a species of photosynthetic bacteria. After separation and purification by silica gel column chromatography and reversed-phase high performance liquid chromatography (RP-HPLC), the four major carotenoids from the superfine powder of R. sphaeroide 3757 were identified as bixin, hydroxyspheroidenone, 3,3¢,4¢-tetrahydrospirilloxanthin-20-al, and spheroidenone by reversed phase - high performance liquid chromatography - atmospheric pressure chemical ionization - mass spectrometry (RP-HPLC-APCI-MS). The antioxidant activity of the carotenoids extracted after superfine grinding of dry biomass of R. sphaeroide 3757 was higher than that after ultrasonic treatment. When the ratio solvent-to-solid was 30, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity, reducing power, and lipid peroxidation inhibitory activity of the extracts after superfine grinding were 74.0% ± 3.1%, 0.497 ± 0.022, and 77.6% ± 3.2%, respectively. By contrast, the extracts after ultrasonic treatment, these numbers were 61.0% ± 2.5%, 0.328 ± 0.014, and 55.2% ± 2.3%, respectively. These results indicate that carotenoids from R. sphaeroide 3757 show a significant antioxidant activity in vitro in a concentration-dependent manner, and that superfine grinding is the optimal extraction method. We hope to provide scientific guidance for commercial production of natural- antioxidant and functional food from carotenoids of R. sphaeroide.

Hyaluronic Acid-Like Substance from Mouse Ovaries with Angiogenic Activity

1990 ◽  
Vol 45 (7-8) ◽  
pp. 873-880 ◽  
Author(s):  
Eimei Sato ◽  
Hajime Miyamoto ◽  
Samuel S. Koide
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Hyaluronic Acid ◽  
Retention Time ◽  
High Performance ◽  
Lateral Wall ◽  
Dependent Manner ◽  
Angiogenic Activity ◽  
Adult Mice ◽  
Dose Dependent

Abstract Glycosaminoglycans prepared from extracts of non-luleal mouse ovaries (JCL-ICR strain) were assayed for neovascularization by implanting Elvax films, impregnated with test samples, on the lateral wall of the sheath of m. rectus abdominis in adult mice of the same strain. Neovascularization occurred in a dose-dependent manner. When purified by chromatography on Dowcx 1-x 2 and DEAE Sephadex columns, fractions eluted with 0.5 M NaCl showed strong ncovascularizing activity. On further purification by high performance liquid chromatography using TSK gel DEAE 2SW column, the fraction with a retention time nearly coincident with that of hyaluronic acid possessed high ncovascularizing activity. The activity of this fraction was markedly reduced when treated with streptococcal hyaluronidase. The present results suggest that glycosaminoglycans, especially a hyaluronic acid-like substance, are involved in ovarian neovascularization.

Development and Validation of A Reversed Phase-High Performance Liquid Chromatography Method for the Quantitative Estimation of Ramipril Drug Content from Formulated Dosage Form

2016 ◽  
Vol 6 (3) ◽  
Author(s):  
Raju Chandra ◽  
Manisha Pant ◽  
Harchan Singh ◽  
Deepak Kumar ◽  
Ashwani Sanghi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Active Ingredient ◽  
High Performance ◽  
Limit Of Detection ◽  
Quantitative Estimation ◽  
Reversed Phase ◽  
Uv Detector ◽  
Chromatography Method ◽  
Drug Content

A reliable and reproducible reversed-phase high performance liquid chromatography (RP-HPLC) was developed for the quantitative determination of Remipril drug content from marketed bulk tablets. The active ingredient of Remipril separation achieved with C18 column using the methanol water mobile phase in the ratio of 40:60 (v/v). The active ingredient of the drug content quantify with UV detector at 215 nm. The retention time of Remipril is 5.63 min. A good linearity relation (R2=0.999) was obtained between drug concentration and average peak areas. The limit of detection and limit of quantification of the instrument were calculated 0.03 and 0.09 µg/mL, respectively. The accuracy of the method validation was determined 102.72% by recoveries method.

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