scholarly journals Changes in free amino acid concentrations and associated gene expression profiles in the abdominal muscle of kuruma shrimp (Marsupenaeus japonicus) acclimated at different salinities

2018 ◽  
Vol 221 (11) ◽  
pp. jeb168997 ◽  
Author(s):  
Hiroki Koyama ◽  
Nanami Mizusawa ◽  
Masataka Hoashi ◽  
Engkong Tan ◽  
Ko Yasumoto ◽  
...  
Keyword(s):  
Gene Expression ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Free Amino ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Abdominal Muscle ◽  
Marsupenaeus Japonicus ◽  
Kuruma Shrimp ◽  
Amino Acid Concentrations

scholarly journals Changes in free amino acid concentrations and associated gene expression profiles in the abdominal muscle of kuruma shrimp Marsupenaeus japonicus reared at different salinity

2017 ◽  
Author(s):  
Hiroki Koyama ◽  
Nanami Mizusawa ◽  
Masataka Hoashi ◽  
Enkong Tan ◽  
Ko Yasumoto ◽  
...  
Keyword(s):  
Amino Acid ◽  
Free Amino Acid ◽  
Free Amino ◽  
Acid Metabolism ◽  
High Salinity ◽  
Abdominal Muscle ◽  
Marsupenaeus Japonicus ◽  
Expression Levels ◽  
Kuruma Shrimp ◽  
Amino Acid Concentrations

AbstractShrimps inhabiting around the coastal area can survive in a wide range of salinity. However, the molecular mechanisms involved in their adaptation to different environmental salinity have remained largely unknown. In the present study, we reared kuruma shrimp Marsupenaeus japonicus at 1.7 %, 3.4 % and 4.0 % salinity. After rearing for 6, 12, 24 and 72 h, we determined free amino acid concentrations in their abdominal muscle, and performed RNA-seq analysis on this muscle. The concentrations of free amino acids were clearly altered depending on salinity after rearing for 24 h. Glutamine and alanine concentrations were markedly increased following the increase of salinity. In association with such changes, many genes related to amino acid metabolism changed their expression levels. Notably, the increased glutamine content at high salinity appeared to be relevant to the increase of the expression level of the gene encoding glutamate-ammonia ligase which functions in the glutamine metabolism. Furthermore, the alanine content increased at high salinity was likely to be associated with the decrease in the expression levels of the alanine-glyoxylate transaminase gene. Thus, the changes in the concentration of free amino acids for osmoregulation in kuruma shrimp are considered to be regulated by the changes in the expression levels of genes related to amino acid metabolism.Summary statesmentKuruma shrimp Marsupenaeus japonicus changes free amino acid contents and associated gene expression levels in their muscle to adjust effectively to different salinity.

Free amino acid concentrations in cerebral cortex of guinea pigs with epileptogenic lesions

1962 ◽  
Vol 5 (6) ◽  
pp. 525-532 ◽  
Author(s):  
Yo Aelony ◽  
John Logothetis ◽  
Bruce Bart ◽  
Frank Morrell ◽  
Magdaline Bovis
Keyword(s):  
Cerebral Cortex ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Guinea Pigs ◽  
Free Amino ◽  
Amino Acid Concentrations

scholarly journals Use of UPLC-ESI-MS/MS to quantitate free amino acid concentrations in micro-samples of mammalian milk

SpringerPlus ◽  
2013 ◽  
Vol 2 (1) ◽  
pp. 622 ◽  
Author(s):  
Véronique Roucher ◽  
Emmanuelle Desnots ◽  
Charlotte Naël ◽  
Aurore Agnoux ◽  
Marie-Cécile Alexandre-Gouabau ◽  
...  
Keyword(s):  
Amino Acid ◽  
Free Amino Acid ◽  
Free Amino ◽  
Esi Ms ◽  
Amino Acid Concentrations
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