scholarly journals Passive regeneration of glutathione: glutathione reductase regulation in the freeze-tolerant North American wood frog,Rana sylvatica

2017 ◽  
Vol 220 (17) ◽  
pp. 3162-3171 ◽  
Author(s):  
Neal J. Dawson ◽  
Kenneth B. Storey
Keyword(s):  
Glutathione Reductase ◽  
North American ◽  
Rana Sylvatica ◽  
Wood Frog ◽  
Freeze Tolerant ◽  
Passive Regeneration

Freeze-thaw injury in erythrocytes of the freeze-tolerant wood frog, Rana sylvatica

1991 ◽  
Vol 261 (6) ◽  
pp. R1346-R1350 ◽  
Author(s):  
J. P. Costanzo ◽  
R. E. Lee
Keyword(s):  
Cell Injury ◽  
Osmotic Fragility ◽  
Rana Sylvatica ◽  
Freeze Tolerance ◽  
Wood Frog ◽  
In Vitro Tests ◽  
Freezing And Thawing ◽  
Freeze Tolerant ◽  
High Concentrations

Erythrocytes from the freeze-tolerant wood frog (Rana sylvatica) were subjected to in vitro tests of freeze tolerance, cryoprotection, and osmotic fragility. The responses of cells from frogs acclimated to 4 or 15 degrees C were similar. Erythrocytes that were frozen in saline hemolyzed at -4 degrees C or lower. The addition of high concentrations (150 and 1,500 mM) of glucose or glycerol, cryoprotectants produced naturally by freeze-tolerant frogs, significantly reduced cell injury at -8 degrees C, but concentrations of 1.5 or 15 mM were ineffective. Hemolysis was reduced by 94% with 1,500 mM glycerol and by 84% with 1,500 mM glucose; thus glycerol was the more effective cryoprotectant. Mean fragility values for frog erythrocytes incubated in hypertonic and hypotonic saline were 1,938 and 49 mosM, respectively. Survival in freeze tolerance and cryoprotection experiments was comparable for erythrocytes from frogs and humans, suggesting that these cells may respond similarly to freezing-related stresses. However, the breadth of osmotic tolerance, standardized for differences in isotonicity, was greater for frog erythrocytes than for human erythrocytes. Our data suggest that erythrocytes from R. sylvatica are adequately protected by glucose under natural conditions of freezing and thawing.

Enzymatic regulation of seasonal glycogen cycling in the freeze-tolerant wood frog, Rana sylvatica

2016 ◽  
Vol 186 (8) ◽  
pp. 1045-1058 ◽  
Author(s):  
M. Clara F. do Amaral ◽  
Richard E. Lee ◽  
Jon P. Costanzo
Keyword(s):  
Rana Sylvatica ◽  
Wood Frog ◽  
Enzymatic Regulation ◽  
Freeze Tolerant

Regulation of pyruvate kinase from muscle of Freeze tolerant wood frog, Rana sylvatica

Cryobiology ◽  
2016 ◽  
Vol 73 (3) ◽  
pp. 437
Author(s):  
J. Mattice ◽  
M. Smolinski ◽  
K. Storey
Keyword(s):  
Pyruvate Kinase ◽  
Rana Sylvatica ◽  
Wood Frog ◽  
Freeze Tolerant
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