Effects of taurine on Ca2(+)-dependent force development of skinned muscle fibre preparations

1990 ◽  
Vol 152 (1) ◽  
pp. 255-264
Author(s):  
S. Galler ◽  
C. Hutzler ◽  
T. Haller
Keyword(s):  
Muscle Fibre ◽  
Isometric Force ◽  
Muscle Fibres ◽  
Force Development ◽  
Skinned Muscle ◽  
Naturally Occurring ◽  
Slow Skeletal Muscle ◽  
Skinned Muscle Fibre ◽  
Skinned Fibre ◽  
Skeletal Muscle Fibres

The effects of the naturally occurring amino acid taurine (2-aminoethanesulphonic acid) on isometric force development were investigated using skinned muscle fibre preparations. In atrial and ventricular pig heart muscles, as well as in fibres of slow abdominal extensor muscle of crayfish, an increase of submaximal isometric force was observed in Ca2(+)-activated skinned fibre preparations at physiological concentrations of taurine. The maximal isometric force remained unaffected in all preparations. It is assumed that taurine increases the Ca2+ sensitivity of the force-generating myofilaments in mammalian hearts and crustacean slow skeletal muscle fibres.

scholarly journals Properties of the ‘slow’ skeletal muscle fibres of the frog*

1953 ◽  
Vol 121 (2) ◽  
pp. 318-340 ◽  
Author(s):  
Stephen W. Kuffler ◽  
E. M. Vaughan Williams
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fibres ◽  
Slow Skeletal Muscle ◽  
Skeletal Muscle Fibres

Comparison of energy release in intact and skinned muscle fibres during development of isometric force

2002 ◽  
Vol 30 (1) ◽  
pp. A35-A35
Author(s):  
T.G. West ◽  
N.A. Curtin ◽  
M.A. Ferenczi ◽  
Z.-H. He ◽  
R.C. Woledge
Keyword(s):  
Energy Release ◽  
Isometric Force ◽  
Muscle Fibres ◽  
Skinned Muscle Fibres ◽  
Skinned Muscle

Two functionally distinct myosin heavy chain isoforms in slow skeletal muscle fibres

FEBS Letters ◽  
1997 ◽  
Vol 410 (2-3) ◽  
pp. 150-152 ◽  
Author(s):  
Stefan Galler ◽  
Karlheinz Hilber ◽  
Bärbel Gohlsch ◽  
Dirk Pette
Keyword(s):  
Skeletal Muscle ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Myosin Heavy Chain Isoforms ◽  
Muscle Fibres ◽  
Slow Skeletal Muscle ◽  
Skeletal Muscle Fibres

Electromechanical Coupling III. Estimation of the Ca Storage Capacity of the SR by Analysing the Time Course of Caffeine- Induced Tension Transients of Skinned Muscle Fibres

1982 ◽  
Vol 37 (7-8) ◽  
pp. 709-711
Author(s):  
Rolf Thieleczek
Keyword(s):  
Time Course ◽  
Electromechanical Coupling ◽  
Storage Capacity ◽  
Muscle Fibres ◽  
Skinned Muscle ◽  
Ca Uptake ◽  
Uptake Activity ◽  
Skinned Fibre ◽  
Tension Transients

Abstract The Ca uptake of the SR of a skinned fibre, which was studied with an indirect method, shows a biphasic time course. A total Ca concentration between 8 and 23 mᴍ was accumulated in the SR by an initial fast process (τ ≈ 4.7 s). This Ca-uptake activity of the SR is high enough to bring about relaxation o f twitch contraction in vivo. The magnitude of a subsequent slow process (τ ≈ 103s) indicates that the total Ca storage capacity of the SR of skeletal muscle is in the order of 60 - 75 mᴍ.

scholarly journals OP0247 IN MYOSITIS MUSCLE FIBRE PLAYS A DIRECT AND CRITICAL ROLE IN THERAPEUTIC RESPONSE TO GLUCOCORTICOIDS

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 151.1-151
Author(s):  
M. Giannini ◽  
G. Laverny ◽  
D. Duteil ◽  
D. Rovito ◽  
A. L. Charles ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Strength ◽  
Muscle Fibre ◽  
Therapeutic Response ◽  
Skeletal Muscle Fibre ◽  
Muscle Fibres ◽  
D 20 ◽  
Grip Test ◽  
Skeletal Muscle Fibres ◽  
Improve Muscle Strength

Background:Myositis are rare autoimmune diseases, affecting more women than men, characterized by chronic inflammation of skeletal muscle causing muscle weakness, decreased quality of life and increased mortality.Glucocorticoids (GC) are potent anti-inflammatory drugs, and are the first line treatment of myositis. They improve muscle strength of myositis patients (therapeutic effect), yet muscle recovery is generally only partial. Moreover, GC have an iatrogenic effect on skeletal muscle fibre leading to steroid myopathy. Thus myositis care has to be improved. Despite the autoimmune terrain of myositis, our team has recently shown that muscle fibres themselves develop immuno-metabolic modifications that participate to muscle weakness and perpetuation of the disease1. GC effects are mediated by the glucocorticoid receptor (GR), which is expressed in various cell types including immune cells and myofibres, but the cells mediating therapeutic responses remain to be determined.Objectives:Unravel the mechanisms underlying the therapeutic effect of GC in myositis, particularly elucidate the role of skeletal muscle fibres.Methods:Experimental myositis was induced in eight to ten week-old C57BL/6J female mice by a single intradermal injection of part of skeletal muscle fast-type C protein along with Freund’s adjuvant and an intraperitoneal (IP) injection of pertussis toxin, as previously described2. Prednisone (PDN) was administered 14 days (D) after the immunization at 1 mg/kg/day for 7 days by gavage. Mice were euthanized 21 days after myositis induction. Muscle strength was assessed by grip test at D 0, before the 1st PDN administration (D 14) and the day before sacrifice (D 20). To investigate whether the PDN effects are mediated by myofibre, we generated transgenic mice carrying two LoxP sites within the GR gene in muscle, expressing the tamoxifen-inducible Cre-ERT2 recombinase selectively in skeletal muscle fibre (HSA-CreERT2/GR L2/L2). Tamoxifen (1 mg/day for 5 days by IP injection) was administered 9 days after immunization to induce GR ablation selectively in skeletal muscle fibres (GR(i)skm-/- mice). Similar treatments were applied to GR L2/L2 that do not express Cre-ER(T2), and served as controls.We compared 4 groups of myositis mice, GR L2/L2 treated by PDN (n=9) or vehicle (n=9) and GR(i)skm-/- treated by PDN (n=10) or vehicle (n=10), by grip test and at the histological level (hematoxylin-eosin (HE) and Gomori trichrome (GT) staining). Moreover, LC3 expression was studied by RTqPCR and western blot.Results:Muscle strength was decreased in both GR L2/L2 and GR(i)skm-/- myositis mice from D 14 to D 20. GR L2/L2 myositis mice recovered muscle strength after PDN treatment; no significant difference compared to D 0 was detected. In contrast, PDN did not improve muscle strength in GR(i)skm-/- myositis mice (Figure 1).HE and GT staining did not reveal quantitative differences in inflammatory infiltrate. Necrotic and degenerative fibres were detected in the 4 groups. At RTqPCR, LC3, an autophagy marker, was upregulated in PDN-treated GR L2/L2 myositis mice compared to untreated GR L2/L2 myositis mice; moreover it was 2-fold more expressed in PDN-treated GR L2/L2 myositis mice compared to PDN-treated GR(i)skm-/- mice.Conclusion:GR in skeletal muscle fibre is crucial to mediate the therapeutic response to GC in a murine model of myositis. Autophagy is one of the candidate pathways controlled by myofibre GR underlying this effect.References:[1]Meyer A et al. IFN-β-induced reactive oxygen species and mitochondrial damage contribute to muscle impairment and inflammation maintenance in dermatomyositis. Acta Neuropathol. 2017 Oct;134(4):655-666.[2]Sugihara T et al. A new murine model to define the critical pathologic and therapeutic mediators of polymyositis. Arthritis Rheum. 2007 Apr;56(4):1304-14.Disclosure of Interests:None declared

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