scholarly journals The thermal dependence of Na+flux in isolated liver cells from ectotherms and endotherms

2016 ◽  
Vol 219 (14) ◽  
pp. 2098-2102 ◽  
Author(s):  
Paul L. Else
Keyword(s):  
Liver Cells ◽  
Thermal Dependence ◽  
Isolated Liver Cells ◽  
Isolated Liver

scholarly journals Arachidonic acid synthesis studied in isolated liver cells

FEBS Letters ◽  
1981 ◽  
Vol 133 (2) ◽  
pp. 201-204 ◽  
Author(s):  
B.O. Christophersen ◽  
Jon Norseth
Keyword(s):  
Arachidonic Acid ◽  
Acid Synthesis ◽  
Liver Cells ◽  
Isolated Liver Cells ◽  
Isolated Liver

Decreased glucagon-stimulated cyclic AMP production by isolated liver cells of rats with type 2 diabetes

1983 ◽  
Vol 32 (1) ◽  
pp. 13-26 ◽  
Author(s):  
Bernard Portha ◽  
Hilda Chamras ◽  
Yvonne Broer ◽  
Luc Picon ◽  
Gabriel Rosselin
Keyword(s):  
Type 2 Diabetes ◽  
Cyclic Amp ◽  
Liver Cells ◽  
Isolated Liver Cells ◽  
Isolated Liver

scholarly journals Amylase in Isolated Liver Cells

1960 ◽  
Vol 235 (5) ◽  
pp. 1354-1358
Author(s):  
Robert L. McGeachin ◽  
Betty Ann Potter
Keyword(s):  
Liver Cells ◽  
Isolated Liver Cells ◽  
Isolated Liver

scholarly journals The role of insulin in the modulation of glucagon-dependent control of phenylalanine hydroxylation in isolated liver cells

1987 ◽  
Vol 242 (3) ◽  
pp. 655-660 ◽  
Author(s):  
M J Fisher ◽  
A J Dickson ◽  
C I Pogson
Keyword(s):  
Cyclic Amp ◽  
Insulin Action ◽  
Phenylalanine Hydroxylase ◽  
Liver Cells ◽  
Phosphorylation State ◽  
Isolated Liver Cells ◽  
The Impact ◽  
Isolated Liver ◽  
Stimulation Of

The stimulation of phenylalanine hydroxylation in isolated liver cells by sub-maximally effective concentrations of glucagon (less than 0.1 microM) is antagonized by insulin (0.1 nM-0.1 microM). This phenomenon is a consequence of a decrease in the glucagon-stimulated phosphorylation of phenylalanine hydroxylase from liver cells incubated in the presence of insulin. The impact of insulin on the phosphorylation state and activity of the hydroxylase is mimicked by incubation of liver cells in the presence of orthovanadate (10 microM). A series of cyclic AMP and cyclic GMP analogues enhanced phenylalanine hydroxylation: in each case insulin diminished the stimulation of flux. These results are discussed in the light of the characteristics of insulin action on other metabolic processes.

scholarly journals Metabolism of 1-3H-Ethanol by Isolated Liver Cells. Time-course of the Transfer of Tritium from R,S-1-3H-Ethanol to Lactate and beta-Hydroxybutyrate.

1976 ◽  
Vol 30b ◽  
pp. 345-352 ◽  
Author(s):  
Niels Grunnet ◽  
Herluf I. D. Thieden ◽  
Bjørn Quistorff ◽  
Michel Devreux ◽  
Jean Vialle ◽  
...  
Keyword(s):  
Time Course ◽  
Liver Cells ◽  
Isolated Liver Cells ◽  
Beta Hydroxybutyrate ◽  
Isolated Liver

Fractionation of isolated liver cells after disruption with a nitrogen bomb and sonication

1982 ◽  
Vol 57 (1) ◽  
pp. 1-13
Author(s):  
F. Autuori ◽  
U. Brunk ◽  
E. Peterson ◽  
G. Dallner
Keyword(s):  
Rat Liver ◽  
Sucrose Gradient ◽  
Divalent Cations ◽  
Liver Cells ◽  
Cell Disruption ◽  
Isolated Liver Cells ◽  
Discontinuous Sucrose Gradient ◽  
Microsomal Membranes ◽  
Isolated Liver

Hepatocytes from rat liver were prepared by perfusion with collagenase, and rough and smooth microsomes and mitochondria were prepared after cell disruption. By applying 1000 lb/in2 (1 lb/in2 = 6.9 kPa) in a nitrogen bomb followed by decompression, 75% of the cells were disrupted after four consecutive treatments. Intact mitochondria, and rough and smooth microsomes with little contamination were prepared from the homogenate. A more rapid disruption was attained by a short sonication with a low output, thus increasing the efficiency of homogenization. The microsomal subfractions prepared from this homogenate were comparable to those obtained after decompression. Sonication resulted in smooth microsomes, which exhibited a higher contamination with non-microsomal membranes. These, however, were partly removed by additional centrifugation with a discontinuous sucrose gradient containing divalent cations.

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