scholarly journals Microtubule-dependent relocation of branchial V-H+-ATPase to the basolateral membrane in the Pacific spiny dogfish (Squalus acanthias): a role in base secretion

2006 ◽  
Vol 209 (4) ◽  
pp. 599-609 ◽  
Author(s):  
M. Tresguerres
Keyword(s):  
Basolateral Membrane ◽  
Squalus Acanthias ◽  
Spiny Dogfish ◽  
The Pacific

Impacts of low salinity exposure and antibiotic application on gut transport activity in the Pacific spiny dogfish, Squalus acanthias suckleyi

2020 ◽  
Vol 190 (5) ◽  
pp. 535-545 ◽  
Author(s):  
Alyssa M. Weinrauch ◽  
Erik J. Folkerts ◽  
Tamzin A. Blewett ◽  
Carol Bucking ◽  
W. Gary Anderson
Keyword(s):  
Squalus Acanthias ◽  
Spiny Dogfish ◽  
Transport Activity ◽  
Low Salinity ◽  
The Pacific

Morphology of the rectal gland of the spiny dogfish (Squalus acanthias) shark in response to feeding

2009 ◽  
Vol 87 (5) ◽  
pp. 440-452 ◽  
Author(s):  
Victoria Matey ◽  
Chris M. Wood ◽  
W. Wesley Dowd ◽  
Dietmar Kültz ◽  
Patrick J. Walsh
Keyword(s):  
Early Stage ◽  
Basolateral Membrane ◽  
Small Diameter ◽  
Squalus Acanthias ◽  
Rectal Gland ◽  
Secretory Cells ◽  
Spiny Dogfish ◽  
Apical Surface ◽  
Stratified Epithelium ◽  
Outer Capsule

The morphology of the rectal gland was examined in spiny dogfish ( Squalus acanthias L, 1758) sharks fasted (1 week) or 6 and 20 h postfeeding. The morphology of the fasted gland showed a pattern reflecting a dormant physiology, with thick gland capsule, thick stratified epithelium, and secretory parenchyma with tubules of small diameter and lumen. The secretory cells of the tubular epithelium were enlarged and irregularly shaped with abnormally condensed or highly vacuolized cytoplasm containing numerous lysosomes. Early-stage apoptotic cells were not uncommon. Secretory cells showed signs of low activity, e.g., mitochondria with weakly stained matrix and small cristae, poorly branched infoldings of basolateral membranes, and microvesicle-free subapical cytoplasm. All characteristics examined changed significantly upon feeding, consistent with increased salt and fluid secretion: the outer capsule muscle layer and the stratified epithelium decreased in diameter; the tubules enlarged; the secretory cells showed extensive development of the basolateral membrane, more mitochondria, and abundant apical microvesicles. Secretory cell apical surface was increased. The minor differences between morphology in 6 and 20 h postfeeding indicated that changes took place rapidly and were complete by 6 h. Our results are discussed in the context of prior studies of metabolism, proteomics, and cellular pathways of gland activation.

Exercise and recovery metabolism in the pacific spiny dogfish ( Squalus acanthias )

2003 ◽  
Vol 173 (6) ◽  
pp. 463-474 ◽  
Author(s):  
J. G. Richards ◽  
G. J. F. Heigenhauser ◽  
C. M. Wood
Keyword(s):  
Squalus Acanthias ◽  
Spiny Dogfish ◽  
The Pacific

Biology and Management of Dogfish Sharks

2009 ◽  
Keyword(s):  
East China Sea ◽  
Pacific Coast ◽  
Japan Sea ◽  
Squalus Acanthias ◽  
Small Scale ◽  
Spiny Dogfish ◽  
Western Coast ◽  
The Pacific ◽  
The Japan Sea ◽  
Hokkaido Island

Abstract.—Around Japan, spiny dogfish <em>Squalus acanthias </em>occur off the Pacific coast of Hokkaido Island and northern Honshu and off the western coast of Japan from the East China Sea to the Japan Sea. They have been caught and used historically on both coasts. This species is usually caught as bycatch except in some small-scale local fisheries which directly target it.

Extracellular carbonic anhydrase and an acid-base disequilibrium in the blood of the dogfish Squalus acanthias

1997 ◽  
Vol 200 (1) ◽  
pp. 173-183 ◽  
Author(s):  
K Gilmour ◽  
R Henry ◽  
C Wood ◽  
S Perry
Keyword(s):  
Carbonic Anhydrase ◽  
Squalus Acanthias ◽  
Co2 Production ◽  
Spiny Dogfish ◽  
Hypoxic Conditions ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Arterial Blood ◽  
The Pacific ◽  
Membrane Bound

The electrometric [Delta]pH method and an in vitro radioisotopic HCO3- dehydration assay were used to demonstrate the presence of true extracellular carbonic anhydrase (CA) activity in the blood of the Pacific spiny dogfish Squalus acanthias. An extracorporeal circulation and stopflow technique were then used to characterise the acid&shy;base disequilibrium in the arterial (postbranchial) blood. During the stopflow period, arterial pH (pHa) decreased by 0.028&plusmn;0.003 units (mean &plusmn; s.e.m., N=27), in contrast to the increase in pHa of 0.029&plusmn;0.006 units (mean &plusmn; s.e.m., N=6) observed in seawater-acclimated rainbow trout Oncorhynchus mykiss under similar conditions. The negative disequilibrium in dogfish blood was abolished by the addition of bovine CA to the circulation, while inhibition by benzolamide of extracellular and gill membrane-bound CA activities reversed the direction of the acid&shy;base disequilibrium such that pHa increased by 0.059&plusmn;0.016 units (mean &plusmn; s.e.m., N=6) during the stopflow period. When the CA activity of red blood cells (rbcs) was additionally inhibited using acetazolamide, the magnitude of the negative disequilibrium was increased significantly to -0.045&plusmn;0.007 units (mean &plusmn; s.e.m., N=6). Blockage of the rbc Cl-/HCO3- exchanger using 4,4'-diisothiocyanostilbene-2,2'-disulphonic acid (DIDS) also increased the magnitude of the negative disequilibrium, in this case to -0.089&plusmn;0.008 units (mean &plusmn; s.e.m., N=6). Exposure of dogfish to hypercapnia had no effect on the disequilibrium, whereas the disequilibrium was significantly larger under hypoxic conditions, at -0.049&plusmn;0.008 units (mean &plusmn; s.e.m., N=6). The results are interpreted within a framework in which the absence of a positive CO2 excretion disequilibrium in the arterial blood of the spiny dogfish is attributed to the membrane-bound and extracellular CA activities. The negative disequilibrium may arise from the continuation of Cl-/HCO3- exchange in the postbranchial blood and/or the hydration of CO2 added to the plasma postbranchially. Two possible sources of this CO2 are discussed; rbc CO2 production or the admixture of blood having 'low' and 'high' CO2 tensions, i.e. the mixing of postbranchial blood with blood which has bypassed the respiratory exchange surface.

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