scholarly journals Aquaporin-3 expressed in the basolateral membrane of gill chloride cells in Mozambique tilapia Oreochromis mossambicus adapted to freshwater and seawater

2005 ◽  
Vol 208 (14) ◽  
pp. 2673-2682 ◽  
Author(s):  
S. Watanabe
Keyword(s):  
Basolateral Membrane ◽  
Oreochromis Mossambicus ◽  
Chloride Cells ◽  
Aquaporin 3 ◽  
Mozambique Tilapia

scholarly journals Mg2+ transport in plasma membrane vesicles of renal epithelium of the Mozambique tilapia (Oreochromis mossambicus)

1997 ◽  
Vol 200 (13) ◽  
pp. 1931-1939 ◽  
Author(s):  
M Bijvelds ◽  
Z Kolar ◽  
S Bonga ◽  
G Flik
Keyword(s):  
Plasma Membrane ◽  
Brush Border ◽  
Transport Mechanism ◽  
Apical Membrane ◽  
Basolateral Membrane ◽  
Membrane Vesicles ◽  
Oreochromis Mossambicus ◽  
Renal Epithelium ◽  
Mozambique Tilapia ◽  
Vesicular Membrane

To elucidate the mechanisms involved in Mg2+ transport at the apical and basolateral poles of the renal tubular epithelium, apical and basolateral plasma membrane vesicle preparations were derived from kidney tissue of freshwater- and seawater-adapted Mozambique tilapia Oreochromis mossambicus. Brush-border preparations were enriched 15.8-fold in alkaline phosphatase activity and consisted almost exclusively of right-side-out membrane vesicles. Basolateral membrane preparations were enriched 7.5-fold in Na+/K+-ATPase activity and contained resealed vesicles and leaky membrane fragments. Mg2+ association with brush-border and basolateral plasma membranes, traced using radioactive 27Mg, occurred in an osmotically active space. In all instances, Mg2+ binding to the vesicular membrane was low compared with the vesicular uptake. Mg2+ equilibration across the vesicular membrane of brush-border preparations was rapid and sensitive to the presence of extravesicular Ca2+, suggesting that the apical membrane of the renal epithelium contains a transport pathway for divalent cations. Application of various ionic gradients did not affect vesicular Mg2+ transport in apical and basolateral membrane preparations, suggesting the presence of an ion-coupled transport mechanism. ATP or ATP--S did not stimulate Mg2+ fluxes, indicating that Mg2+ transport does not proceed via an ATP-driven or activated transporter. In these aspects, vesicular Mg2+ transport was similar in seawater and freshwater preparations. These results suggest that the apical membrane of renal epithelial cells lacks an active secretory Mg2+ transport mechanism. We propose that the Mg2+ conductivity of the apical membrane reflects a route for downhill Mg2+ entry and is involved in renal Mg2+ reabsorption.

scholarly journals Effects of 17ß-estradiol Hormone on Growth and Sex Differentiation in the Mozambique Tilapia (Oreochromis mossambicus, Peters, 1852)

2019 ◽  
Vol 34 (1) ◽  
pp. 14-21
Author(s):  
Karl Christofer Kingueleoua Koyakomanda ◽  
Muamer Kürşat Fırat ◽  
Cüneyt Süzer ◽  
Serhat Engin ◽  
Müge Hekimoğlu ◽  
...  
Keyword(s):  
Sex Differentiation ◽  
Oreochromis Mossambicus ◽  
Mozambique Tilapia

scholarly journals clc-2c is regulated by salinity, prolactin and extracellular osmolality in tilapia gill

2017 ◽  
Vol 59 (4) ◽  
pp. 391-402 ◽  
Author(s):  
Jason P Breves ◽  
Paige L K Keith ◽  
Bethany L Hunt ◽  
K Keano Pavlosky ◽  
Mayu Inokuchi ◽  
...  
Keyword(s):  
Danio Rerio ◽  
External Environment ◽  
Oreochromis Mossambicus ◽  
Local Effect ◽  
Intrinsic Factors ◽  
Mozambique Tilapia ◽  
Regulatory Link ◽  
Gill Filaments ◽  
Ovine Prolactin ◽  
First Time

Teleosts inhabiting fresh water (FW) depend upon ion-absorptive ionocytes to counteract diffusive ion losses to the external environment. A Clc Cl−channel family member, Clc-2c, was identified as a conduit for basolateral Cl−transport by Na+/Cl−cotransporter 2 (Ncc2)-expressing ionocytes in stenohaline zebrafish (Danio rerio). It is unresolved whether Clc-2c/clc-2cis expressed in euryhaline species and how extrinsic and/or intrinsic factors modulate branchialclc-2cmRNA. Here, we investigated whether environmental salinity, prolactin (Prl) and osmotic conditions modulateclc-2cexpression in euryhaline Mozambique tilapia (Oreochromis mossambicus). Branchialclc-2candncc2mRNAs were enhanced in tilapia transferred from seawater (SW) to FW, whereas both mRNAs were attenuated upon transfer from FW to SW. Next, we injected hypophysectomized tilapia with ovine prolactin (oPrl) and observed a marked increase inclc-2cfrom saline-injected controls. To determine whether Prl regulatesclc-2cin a gill-autonomous fashion, we incubated gill filaments in the presence of homologous tilapia Prls (tPrl177and tPrl188). By 24 h, tPrl188stimulatedclc-2cexpression ~5-fold from controls. Finally, filaments incubated in media ranging from 280 to 450 mosmol/kg for 3 and 6 h revealed that extracellular osmolality exerts a local effect onclc-2cexpression;clc-2cwas diminished by hyperosmotic conditions (450 mosmol/kg) compared with isosmotic controls (330 mosmol/kg). Our collective results suggest that hormonal and osmotic control of branchialclc-2ccontributes to the FW adaptability of Mozambique tilapia. Moreover, we identify for the first time a regulatory link between Prl and a Clc Cl−channel in a vertebrate.

scholarly journals Ambient Salinity Modulates the Expression of Sodium Pumps in Branchial Mitochondria-Rich Cells of Mozambique Tilapia, Oreochromis mossambicus

2003 ◽  
Vol 20 (1) ◽  
pp. 29-36 ◽  
Author(s):  
Tsung-Han Lee ◽  
Shin-Huey Feng ◽  
Chia-Hao Lin ◽  
Yu-Hwa Hwang ◽  
Chao-Lu Huang ◽  
...  
Keyword(s):  
Oreochromis Mossambicus ◽  
Sodium Pumps ◽  
Mozambique Tilapia ◽  
Ambient Salinity
Sign in / Sign up

Export Citation Format

Share Document