scholarly journals Three-dimensional antennal lobe atlas of male and female moths, Lobesia botrana (Lepidoptera: Tortricidae) and glomerular representation of plant volatiles in females

2005 ◽  
Vol 208 (6) ◽  
pp. 1147-1159 ◽  
Author(s):  
I. Masante-Roca
2002 ◽  
Vol 205 (10) ◽  
pp. 1363-1376 ◽  
Author(s):  
Medhat M. Sadek ◽  
Bill S. Hansson ◽  
Jean Pierre Rospars ◽  
Sylvia Anton

SUMMARY We studied the projection patterns of antennal lobe (AL) interneurones sensitive to plant volatiles and female-produced sex pheromone components in the female moth, Spodoptera littoralis. Ten compounds (eight plant-derived compounds and two sex pheromone components) were singly applied to the antenna and, using intracellular recording and staining techniques, the physiological and morphological characteristics of responding neurones were investigated. In addition, ALs stained with a synapsin antibody were optically sectioned using confocal microscopy, and a three-dimensional map of glomeruli in the anterior aspect of the AL was reconstructed. We used the map as a reference for identification of glomeruli innervated by projection neurones(PNs) that respond to plant volatiles and/or pheromone components. Nineteen PNs, responding to one to seven compounds of the ten tested stimuli, were stained with neurobiotin. These neurones each arborised in a single glomerulus in the frontal side of the AL. PNs responding to the same compound arborised in different glomeruli and PNs arborising in the same glomerulus responded to different compounds. Accordingly, glomeruli harbouring the dendritic arborisations of PNs responding to each of the tested compounds constituted a unique array of glomeruli that were not necessarily adjacent. It was thus clear that, at the output level, a single plant volatile or a sex pheromone component was not represented within a single glomerulus in the AL. We expect complex patterns of glomeruli to be involved in the coding of plant-derived compounds, as well as sex pheromone components, in female S. littoralis.


2009 ◽  
Vol 337 (3) ◽  
pp. 513-526 ◽  
Author(s):  
Nélia Varela ◽  
Louise Couton ◽  
César Gemeno ◽  
Jesús Avilla ◽  
Jean-Pierre Rospars ◽  
...  

2016 ◽  
Vol 113 ◽  
pp. 579-586 ◽  
Author(s):  
Albert SANS ◽  
Miguel MORAN ◽  
Magi RIBA ◽  
Angel GUERRERO ◽  
Jaume ROIG ◽  
...  

2017 ◽  
Vol 16 (3) ◽  
pp. 313-324 ◽  
Author(s):  
Jaymee R. Shell ◽  
Shawn M. K. Robbins ◽  
Philippe C. Dixon ◽  
Philippe J. Renaud ◽  
René A. Turcotte ◽  
...  

2012 ◽  
Vol 38 (2) ◽  
pp. 222-225 ◽  
Author(s):  
Martin von Arx ◽  
Daniela Schmidt-Büsser ◽  
Patrick M. Guerin

1995 ◽  
Vol 350 (1333) ◽  
pp. 235-242 ◽  

We are interested in understanding how the field of cells which forms the gonad arises, and how the testis-determining gene, Sry , controls morphogenesis of a testis within this field of cells. To appreciate changes in the three-dimensional structure of the mouse genital ridge at this time in development, whole-mount genital ridges taken from male and female embryos over the developmental period when the initiation of testis cord morphogenesis takes place, were stained with an antibody against laminin. Samples were visualized using confocal microscopy. Anti-laminin illuminates the elaborate array of mesonephric duct and tubules which occupy the cranial two-thirds of the mesonephros at the earliest timepoint. This complex structure gradually regresses as testis cords form in male gonads. No structural organisation is recognized by this antibody in the female gonadal region during this period. Confocal sections in the Z-plane reveal continuous cellular connections between 3-6 mesonephric tubules and the gonadal primordium. These cellular bridges are present in male and female gonads, so they do not depend on the expression of Sry . We consider the possibility that these bridges constitute the pathways of the founder cells of the gonadal primordium.


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