scholarly journals Inhibition of the increased 17 -estradiol-induced mast cell number by melatonin in the testis of the frog Rana esculenta, in vivo and in vitro

2004 ◽  
Vol 207 (3) ◽  
pp. 437-441 ◽  
Author(s):  
G. Izzo
Keyword(s):  
Mast Cell ◽  
Rana Esculenta ◽  
Cell Number ◽  
Mast Cell Number

Effects of sex steroid hormones and their antagonists on mast cell number in the testis of the frog, Rana esculenta

Zygote ◽  
2000 ◽  
Vol 8 (3) ◽  
pp. 225-234 ◽  
Author(s):  
L. Di Matteo ◽  
I. Izzo Vitiello ◽  
S. Minucci
Keyword(s):  
Mast Cell ◽  
Cyproterone Acetate ◽  
Leydig Cells ◽  
Androgen Receptors ◽  
Rana Esculenta ◽  
Cell Number ◽  
Physiological Effect ◽  
Sex Steroid ◽  
Mast Cell Number ◽  
Germinal Cells

This study confirms our previous data on the effects of sex hormones on mast cell number (MCN) in the testis of frog Rana esculenta. After 15 days of treatment with oestradiol (E2) MCN strongly increases, while testosterone has no effect. After 30 days only a small increase in MCN is observed. These differences could be due to the non-physiological effect of E2 over a prolonged period. We also confirmed a massive increase in MCN after 15 or 30 days of treatment with cyproterone acetate (CPA). This increase in MCN is also observed after administration of CPA with tamoxifen. Ultrastructural analysis of testis shows empty spaces with degenerating Leydig cells in the interstitial compartment and numerous germinal cells completely degenerated, probably apoptotic, in the adjacent germinal compartment. The same effects were observed in testes after treatment with only CPA. Chronic E2 treatment provokes an increase in MCN on day 2. From day 4 to 12 of the treatment, MCN decreases dramatically and many germinal tubules appear strongly disorganised. In conclusion, the present results confirm that E2 treatment induces changes in MCN and chronic E2 treatment modifies the morphology of the frog testes. In addition, blocking androgen receptors with CPA, alone or in combination with tamoxifen, causes a significant increase in MCN, confirming the involvement of androgens in mast cell proliferation and/or differentiation.

The Effects of Testosterone and Estradiol on Mast Cell Number in the Harderian Gland of the Frog, Rana esculenta

1995 ◽  
Vol 12 (4) ◽  
pp. 457-466 ◽  
Author(s):  
Loredana Di Matteo ◽  
Gabriella Chieffi Baccari ◽  
Paolo Chieffi ◽  
Sergio Minucci
Keyword(s):  
Mast Cell ◽  
Harderian Gland ◽  
Rana Esculenta ◽  
Cell Number ◽  
Mast Cell Number

17β-estradiol effects on mast cell number and spermatogonial mitotic index in the testis of the frog,Rana esculenta

1997 ◽  
Vol 278 (2) ◽  
pp. 93-100 ◽  
Author(s):  
Sergio Minucci ◽  
Loredana di Matteo ◽  
Paolo Chieffi ◽  
Riccardo Pierantoni ◽  
Silvia Fasano
Keyword(s):  
Mast Cell ◽  
Mitotic Index ◽  
Rana Esculenta ◽  
Cell Number ◽  
17Β Estradiol ◽  
Mast Cell Number

scholarly journals IL-10 Suppresses Mast Cell IgE Receptor Expression and Signaling In Vitro and In Vivo

2008 ◽  
Vol 180 (5) ◽  
pp. 2848-2854 ◽  
Author(s):  
Sarah Kennedy Norton ◽  
Brian Barnstein ◽  
Jennifer Brenzovich ◽  
Daniel P. Bailey ◽  
Mohit Kashyap ◽  
...  
Keyword(s):  
Mast Cell ◽  
Receptor Expression ◽  
Ige Receptor

Opioids and testicular activity in the frog, Rana esculenta

1993 ◽  
Vol 137 (1) ◽  
pp. 49-NP ◽  
Author(s):  
F. Facchinetti ◽  
A. R. Genazzani ◽  
M. Vallarino ◽  
M. Pestarino ◽  
A. Polzonetti-Magni ◽  
...  
Keyword(s):  
Physiological Activity ◽  
Mammalian Species ◽  
Rana Esculenta ◽  
Cell Degeneration ◽  
Efferent System ◽  
Naltrexone Treatment ◽  
Nucleus Infundibularis ◽  
The Brain

ABSTRACT The presence and activity of brain, pituitary and testicular β-endorphin (β-EP)-like material have been studied in the frog, Rana esculenta, using reverse-phase high-pressure liquid chromatography, coupled with radioimmunoassay and immunocytochemistry. In-vivo and in-vitro treatments with naltrexone were carried out to assess the putative physiological activity of opioid peptides. β(1–31) and (1–27), together with their acetylated forms, have been identified in brain, pituitary and testis. In particular, β-EP(1–31) concentrations peaked during July in the brain and pituitary, whilst in testes maximum concentrations were found in April and November. β-EP immunoreactivity was present in the brain within the nucleus preopticus and nucleus infundibularis ventralis while positive fibres in the retrochiasmatic regions projected to the median eminence. In the testis, interstitial cells, canaliculi of the efferent system, spermatogonia and spermatocytes showed positive immunostaining for β-EP. In intact animals, naltrexone treatment increased plasma and testicular androgen levels and this effect was confirmed in in-vitro incubations of minced testes. Naltrexone also induced a significant increase in germ cell degeneration. Our results indicated that an opioid system modulates the hypothalamus-pituitary-gonadal axis in the frog, Rana esculenta and, for the first time, we have shown that the testicular activity of a non-mammalian species may be regulated by opiates locally. Journal of Endocrinology (1993) 137, 49–57

scholarly journals GPR30 decreases cardiac chymase/angiotensin II by inhibiting local mast cell number

2015 ◽  
Vol 459 (1) ◽  
pp. 131-136 ◽  
Author(s):  
Zhuo Zhao ◽  
Hao Wang ◽  
Marina Lin ◽  
Leanne Groban
Keyword(s):  
Mast Cell ◽  
Angiotensin Ii ◽  
Cell Number ◽  
Mast Cell Number

The expulsion of Echinostoma trivolvis: worm kinetics and intestinal cytopathology in conventional and congenitally athymic BALB/c mice

Parasitology ◽  
1993 ◽  
Vol 106 (3) ◽  
pp. 297-304 ◽  
Author(s):  
T. Fujino ◽  
B. Fried ◽  
I. Tada
Keyword(s):  
Mast Cell ◽  
Goblet Cells ◽  
Cell Number ◽  
Athymic Mice ◽  
Post Exposure ◽  
Mucosal Mast Cells ◽  
Worm Recovery ◽  
Mast Cell Number ◽  
Echinostoma Trivolvis ◽  
Over Time

SUMMARYThe infectivity and distribution of Echinostoma trivolvis were studied in male, conventional and congenitally athymic nude mice, each infected with 30 metacercarial cysts. In conventional mice, worm recoveries at 6 and 8 days post-exposure were58·3 and 54·0%, respectively. Worm recovery declined to 44·0% by day 10, to 4·3% by day 13, and 0% by day 17. In athymic mice, worm recoveries at 6 and 8 days post-exposure were 61·7 and 36·3%, respectively. Worm recovery declined to 27·7% by day 10, to 0·7% by day 13, and 0% by day 17. The distribution of worms demonstrated a posteriad migration over time in both groups. Kinetic changes in the number of goblet and mucosal mast cells in the upper ileum of mice infected with E. trivolvis were examined. In conventional mice, the number of goblet cells increased rapidly to reach a peak at day 13 and then declined gradually. The number of goblet cells in athymic mice also increased to reach a peak at day 13, and then declined rapidly. However, the number of goblet cells in athymic mice was always less than that inconventional mice. The mast cell number in infected conventional mice increased rapidly to reach a peak at day 17 and then declined. There was no increase in the mast cell number of infected athymic mice throughout the experiment. Whereas common pathological changes occurred in the intestines of both mice groups infected with echinostomes some ultrastructural differences were observed in the gut epithelial cells of conventional versus athymic mice.

Effect of Chicoric Acid on Mast Cell-Mediated Allergic Inflammation in Vitro and in Vivo

2015 ◽  
Vol 78 (12) ◽  
pp. 2956-2962 ◽  
Author(s):  
Na Young Lee ◽  
Kyung-Sook Chung ◽  
Jong Sik Jin ◽  
Keuk Soo Bang ◽  
Ye-Jin Eom ◽  
...  
Keyword(s):  
Mast Cell ◽  
Allergic Inflammation ◽  
Chicoric Acid

The preimplantation pig embryo: cell number and allocation to trophectoderm and inner cell mass of the blastocyst in vivo and in vitro

Development ◽  
1988 ◽  
Vol 102 (4) ◽  
pp. 793-803 ◽  
Author(s):  
V.E. Papaioannou ◽  
K.M. Ebert
Keyword(s):  
Inner Cell Mass ◽  
Cell Mass ◽  
Staining Technique ◽  
Cell Number ◽  
Total Cell ◽  
Differential Staining ◽  
Morphological Development ◽  
Total Cell Number

Total cell number as well as differential cell numbers representing the inner cell mass (ICM) and trophectoderm were determined by a differential staining technique for preimplantation pig embryos recovered between 5 and 8 days after the onset of oestrus. Total cell number increased rapidly over this time span and significant effects were found between embryos of the same chronological age from different females. Inner cells could be detected in some but not all embryos of 12–16 cells. The proportion of inner cells was low in morulae but increased during differentiation of ICM and trophectoderm in early blastocysts. The proportion of ICM cells then decreased as blastocysts expanded and hatched. Some embryos were cultured in vitro and others were transferred to the oviducts of immature mice as a surrogate in vivo environment and assessed for morphology and cell number after several days. Although total cell number did not reach in vivo levels, morphological development and cell number increase was sustained better in the immature mice than in vitro. The proportion of ICM cells in blastocysts formed in vitro was in the normal range.

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