scholarly journals Localisation of VIP-binding sites exhibiting properties of VPAC receptors in chromaffin cells of rainbow trout (Oncorhynchus mykiss)

2003 ◽  
Vol 206 (11) ◽  
pp. 1917-1927 ◽  
Author(s):  
C. J. Montpetit
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Binding Sites ◽  
Chromaffin Cells ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Vpac Receptors

scholarly journals An in-vitro study of Himalayan plant extracts against oomycetes disease Saprolegniasis in rainbow trout (Oncorhynchus mykiss)

2021 ◽  
Vol 42 (4) ◽  
pp. 1008-1018
Author(s):  
R.S. Tandel ◽  
◽  
N.K. Chadha ◽  
P. Dash ◽  
P.B. Sawant ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Binding Sites ◽  
Effector Proteins ◽  
Mycelium Growth ◽  
Butea Monosperma ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Target Binding ◽  
Myrica Esculenta

Aim: This study aimed to investigate the effectiveness of ethanolic extract of three Himalayan plants Myrica esculenta, Thymus linearis and Butea monosperma on hyphal germination, colonisation and sporulation of two species of Saprolegnia (Saprolegnia parasitica and S. australis) isolated from rainbow trout, Oncorhynchus mykiss. Molecular docking of active ingredients of M. esculenta, Myricetin with effector proteins of S. parasitica was also performed to investigate the target binding sites for drug development. Methodology: Minimum inhibitory concentration (MIC), mycelium growth inhibition, spore germination, and inhibition was performed with the most effective concentrations. Molecular docking was carried out with AutoDock Vina software to investigate target binding sites with S. parasitica. Results: Extracts from Myrica esculenta, Thymus linearis and Butea monosperma showed MIC values of the 25, 100, 50 mg ml-1 against S. parasitica and 25, 50, 25 mg ml-1 against S. australis hyphal growth, respectively. Nevertheless, malachite green as reference control was effective with a MIC value of 2.5 mg l-1. The concentration required to inhibit S. parasitica and S. australis spores were (50) Myrica esculenta, (25) Thymus linearis, (100) Butea monosperma in mg ml-1 and (50) Myrica esculenta, (50) Thymus linearis, (100) Butea monosperma in mg ml-1, respectively. Interpretation: The study concludes that M. esculenta and B. monosperma are effective against Saprolegniasis and could be used as phyto additives.

scholarly journals SEROTONIN-MEDIATED RELEASE OF CATECHOLAMINES IN THE RAINBOW TROUT ONCORHYNCHUS MYKISS

1993 ◽  
Vol 178 (1) ◽  
pp. 191-204 ◽  
Author(s):  
R. Fritsche ◽  
S. G. Reid ◽  
S. Thomas ◽  
S. F. Perry
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Chromaffin Cells ◽  
Head Kidney ◽  
Catecholamine Release ◽  
Perfusion Fluid ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Dose Dependent

The effects of serotonin (5-hydroxytryptamine; 5-HT) on catecholamine release from chromaffin tissue were investigated in the rainbow trout (Oncorhynchus mykiss) in vivo and in situ. Intra-arterial injections of serotonin in vivo caused dose-dependent (50–250 nmol kg-1) increases in both plasma noradrenaline and adrenaline levels. Pre-treatment of fish with the serotonergic receptor antagonist methysergide did not abolish these increases. An in situ saline-perfused head kidney preparation was developed and validated to study the potential direct effect of serotonin on catecholamine release. The chromaffin cells in the preparation showed a dose-dependent release of catecholamines in response to bolus injections of the cholinergic receptor agonist carbachol (10–7-10-4 mol kg-1). The carbachol-induced release of noradrenaline, but not of adrenaline, was reduced significantly when the nicotinic receptor antagonist hexamethonium (10–4 mol l-1) was present in the perfusion fluid. The removal of calcium from the perfusion fluid prevented the usual release of catecholamines evoked by carbachol. Bolus injections of serotonin (250 nmol kg-1) into the inflowing perfusion fluid resulted in significantly increased levels of adrenaline and noradrenaline in the outflowing perfusate. Addition of hexamethonium to the perfusion fluid did not abolish this serotonin-induced release of catecholamines. The serotonin-induced release of adrenaline, however, was abolished totally by the addition of methysergide. Serotonin is present in high concentrations (44.61+/−5.96 microgram g-1 tissue) in the anterior region of the posterior cardinal vein within the head kidney. Carbachol (10–5 mol kg-1) did not elicit release of the stored serotonin from the perfused head kidney preparation. We conclude that the chromaffin cells in the perfused trout head kidney preparation display characteristics similar to those of other vertebrates and that this preparation is a useful tool for studying the control of catecholamine release in fish. The results demonstrate that serotonin has a direct impact on the chromaffin cells by interacting with methysergide-sensitive receptors to initiate the release of adrenaline. The potential physiological role of serotonin on catecholamine release in trout is discussed.

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