REVIEW: Molecular and cellular approaches to the analysis of plant embryo development from somatic cells in vitro

1975 ◽  
Vol 99 (3) ◽  
pp. 473-482
Author(s):  
DNES DUDITS ◽  
LSZL BGRE ◽  
JNOS GYRGYEY
Keyword(s):  
Embryo Development ◽  
Somatic Cells ◽  
Plant Embryo ◽  
Plant Embryo Development

scholarly journals GENE EXPRESSION DURING INDIRECT SOMATIC EMBRYOGENESIS OF PLANTS

2004 ◽  
Vol 42 (2) ◽  
Author(s):  
Tammy Estabrooks ◽  
Zhongmin Dong
Keyword(s):  
Gene Expression ◽  
Somatic Embryogenesis ◽  
Embryo Development ◽  
Current Literature ◽  
Molecular Mechanisms ◽  
Indirect Somatic Embryogenesis ◽  
Experimental Tool ◽  
Plant Embryo ◽  
Plant Embryo Development

Somatic embryogenesis is the process by which somatic cells are induced into an embryogenic state, followed by differentiation into embryos. Somatic embryogenesis, in addition to being a method of propagation, can serve as an experimental tool for research into plant embryo development. This is a review of the current literature on in vitro plant somatic embryogenesis and the molecular advances made to identify genes expressed during the various stages of this process. Some factors hindering the elucidation of the molecular mechanisms underlying somatic embryogenesis are discussed.L’embryogenèse somatique est le processus par lequel les cellules somatiques passent à l’état embryogène et se différencient en embryons. En plus de constituer une méthode de propagation, elle peut servir d’outil expérimental de recherche pour développer des embryons de plantes. Le présent document est une revue de la documentation sur l’embryogenèse somatique végétale in vitro et sur les progrès réalisés à l’échelle moléculaire pour identifier les gènes exprimés au cours des divers stades du processus. On examine aussi certains facteurs qui rendent difficile l’élucidation des mécanismes moléculaires de l’embryogenèse somatique.

scholarly journals Inactivation of a Glycyl-tRNA Synthetase Leads to an Arrest in Plant Embryo Development

1998 ◽  
Vol 10 (8) ◽  
pp. 1277-1294 ◽  
Author(s):  
Ursula Uwer ◽  
Lothar Willmitzer ◽  
Thomas Altmann
Keyword(s):  
Embryo Development ◽  
Trna Synthetase ◽  
Plant Embryo ◽  
Plant Embryo Development

scholarly journals Embryo coculture: A review

2015 ◽  
Vol 2 (1) ◽  
pp. 15 ◽  
Author(s):  
Alexis P. Melnick ◽  
Steven D. Spandorfer
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Development ◽  
Somatic Cells ◽  
Pregnancy Rates ◽  
Vitro Fertilization

Since the inception of in-vitro fertilization, various attempts have been made to improve in-vitro embryo development, therebyenhancing implantation and in turn, pregnancy rates. Coculture, the simultaneous culture of somatic cells together with theembryo, is one strategy that has been used to achieve this goal. We review the rationale behind the technique of coculture, currentmethods utilized, and the evidence supporting its use.

scholarly journals Emerging role of extracellular vesicles in communication of preimplantation embryos in vitro

2017 ◽  
Vol 29 (1) ◽  
pp. 66 ◽  
Author(s):  
Krishna C. Pavani ◽  
Carmen Alminana ◽  
Eline Wydooghe ◽  
Maaike Catteeuw ◽  
Miguel A. Ramírez ◽  
...  
Keyword(s):  
Embryo Development ◽  
Extracellular Vesicles ◽  
Molecular Mechanisms ◽  
Culture Media ◽  
Somatic Cells ◽  
Indirect Evidence ◽  
Preimplantation Embryos ◽  
Limited Information ◽  
Paracrine Factors

In vitro, efficient communication between mammalian embryos in groups or between embryos and cocultured somatic cells implies that there is a sender, a message and a receiver that is able to decode the message. Embryos secrete a variety of autocrine and paracrine factors and, of these, extracellular vesicles have recently been implicated as putative messengers in embryo–embryo communication, as well as in communication of the embryo with the maternal tract. Extracellular vesicles (EVs) are membrane-bound vesicles that are found in biofluids and in culture media conditioned by the presence of embryos or cells. EVs carry and transfer regulatory molecules, such as microRNAs, mRNAs, lipids and proteins. We conducted a systematic search of the literature to review and present the currently available evidence regarding the possible roles of EVs in in vitro embryo communication and embryo development. It is important to note that there is limited information available on the molecular mechanisms and many of the biologically plausible functions of EVs in embryo communication have not yet been substantiated by conclusive experimental evidence. However, indirect evidence, such as the use of media conditioned by embryos or by somatic cells with improved embryo development as a result, may indicate that EVs can be an important asset for the development of tailor-made media, allowing better embryo development in vitro, even for single embryo culture.

scholarly journals Inactivation of a Glycyl-tRNA Synthetase Leads to an Arrest in Plant Embryo Development

1998 ◽  
Vol 10 (8) ◽  
pp. 1277
Author(s):  
Ursula Uwer ◽  
Lothar Willmitzer ◽  
Thomas Altmann
Keyword(s):  
Embryo Development ◽  
Trna Synthetase ◽  
Plant Embryo ◽  
Plant Embryo Development

scholarly journals A cornucopia of mutants for understanding plant embryo development

2020 ◽  
Vol 226 (2) ◽  
pp. 289-291
Author(s):  
María Rosa Ponce ◽  
José Luis Micol
Keyword(s):  
Embryo Development ◽  
Plant Embryo ◽  
Plant Embryo Development
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