A role for preprophase bands of microtubules in maturation of new cell walls, and a general proposal on the function of preprophase band sites in cell division in higher plants

1990 ◽  
Vol 97 (3) ◽  
pp. 527-537
Author(s):  
Y. MINEYUKI ◽  
B. E. S. GUNNING
Keyword(s):  
Cell Division ◽  
Hair Cells ◽  
Cell Walls ◽  
Higher Plants ◽  
Preprophase Band ◽  
Cell Plate ◽  
Time Lapse ◽  
Spatial Regulation ◽  
Plant Cell Division ◽  
Drug Treatments

Time-lapse video microscopy of dividing Tradescantia stamen hair cells that are undergoing cytokinesis has revealed that the maturation of the new cell wall is aided by factors at the site where the preprophase band of microtubules forms before mitosis. The wall changes from being fluid and wrinkled before it is inserted into the parental wall at the end of cytokinesis, to being stiff and flat by about 20 min after the time of attachment. This change occurs only if the new wall is inserted at the site formerly occupied by the preprophase band. The cell plate does not flatten when it is caused to insert elsewhere by drug treatments or by centrifugal displacement. If insertion at the correct site is delayed locally by centrifugation against the direction of expansion of the cell plate, then flattening is delayed at the same locality. In combination with a number of points from the literature of plant cell division, some of them very long-standing, our observations lead to a general proposal regarding the nature of the preprophase band site, its mode of action and timing of its operations, and how its role in spatial regulation of histogenesis is achieved.

scholarly journals Dynamic Apical-Basal Enrichment of the F-Actin during Cytokinesis in Arabidopsis Cells Embedded in Their Tissues

2021 ◽  
Author(s):  
Alexis Lebecq ◽  
Aurelie Fangain ◽  
Alice Boussaroque ◽  
Marie-Cecile Caillaud
Keyword(s):  
Cell Division ◽  
Tracking System ◽  
Preprophase Band ◽  
Cell Plate ◽  
Tumor Formation ◽  
Actin Dynamics ◽  
Root Cells ◽  
Developmental Defects ◽  
Plant Cell Division ◽  
Dividing Cells

During the life cycle of any multicellular organism, cell division contributes to the proliferation of the cell in the tissues as well as the generation of specialized cells, both necessary to form a functional organism. Therefore, the mechanisms of cell division need to be tightly regulated, as malfunctions in their control can lead to tumor formation or developmental defects. This is particularly true in land plants, where cells cannot relocate and therefore cytokinesis is key for morphogenesis. In the green lineage, cell division is executed in radically different manners than animals, with the appearance of new structures (the preprophase band (PPB), cytokinetic the cell plate and phragmoplast), and the disappearance of ancestral mechanisms (cleavage, centrosomes). While F-actin and microtubules closely co-exist to allow the orientation and the progression of the plant cell division, recent studies mainly focused on the involvement of microtubules in this key process. Here, we used our recently developed root tracking system to follow actin dynamics in dividing Arabidopsis meristematic root cells. In this study, we imaged in time and space the fluorescent-tagged F-actin reporter Lifeact together with cell division markers in dividing cells embedded in their tissues. In addition to the F-actin accumulation in the phragmoplasts, we observed and quantified a dynamic apical-basal enrichment of the F-actin during cytokinesis. The role and the possible actors responsible for F-actin dynamics during cytokinesis are discussed.

scholarly journals Cytokinesis in fra2 Arabidopsis thaliana p60-katanin Mutant: Defects in Cell Plate/Daughter Wall Formation

2021 ◽  
Author(s):  
Emmanuel Panteris ◽  
Anna Kouskouveli ◽  
Dimitris Pappas ◽  
Ioannis-Dimosthenis S. Adamakis
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Wall ◽  
Cell Walls ◽  
Higher Plants ◽  
Cell Plate ◽  
Wild Type ◽  
Microtubule Organization ◽  
Root Cells ◽  
Wall Formation ◽  
In The Wild

Cytokinesis is accomplished in higher plants by the phragmoplast, creating and conducting the cell plate, to separate daughter nuclei by a new cell wall. The microtubule-severing enzyme p60-katanin plays an important role in the centrifugal expansion and timely disappearance of phragmoplast microtubules. Consequently, aberrant structure and delayed expansion rate of the phragmoplast occur in p60-katanin mutants. Here, the consequences of p60-katanin malfunction in cell plate/daughter wall formation were investigated by transmission electron microscopy (TEM), while deviations in the chemical composition of cell plate/new cell wall were identified by immunolabeling and confocal microscopy, in root cells of the fra2 Arabidopsis thaliana mutant. It was found that, apart from defective phragmoplast microtubule organization, cell plates/new cell walls appeared also faulty in structure, being unevenly thick and perforated by large gaps. In addition, demethylesterified homogalacturonans were prematurely present in fra2 cell plates, while callose content was significantly lower than in the wild-type. Furthermore, KNOLLE syntaxin disappeared from newly formed cell walls in fra2 earlier than in the wild-type. Taken together, these observations indicate that delayed cytokinesis, due to faulty phragmoplast organization and expansion, results in a loss of synchronization between cell plate growth and its chemical maturation.

Electron microscope study of vegetative cell division in two species of Marchanda

1978 ◽  
Vol 56 (5) ◽  
pp. 467-475 ◽  
Author(s):  
Larry C. Fowke ◽  
Jeremy D. Pickett-Heaps
Keyword(s):  
Electron Microscope ◽  
Cell Division ◽  
Microscope Study ◽  
Vegetative Cell ◽  
Electron Microscope Study ◽  
Higher Plants ◽  
Cell Plate ◽  
Early Prophase ◽  
Higher Plant ◽  
Spindle Microtubules

Cell division in Marehantia polymorpha and M. berteroana was examined with the electron microscope. Distinct preprophase bands of microtubules, typical of higher plants, were not observed. Most of the spindle microtubules in early prophase appeared to insert into polar MTOC's. The behaviour of the nuclear envelope, nucleolus, and chromosomes was typical of higher plant divisions. Cytokinesis was accomplished by centrifugal cell plate growth in a phragmoplast. Numerous coated vesicles were associated with the developing cell plate.

scholarly journals Cytokinesis in fra2 Arabidopsis thaliana p60-Katanin Mutant: Defects in Cell Plate/Daughter Wall Formation

2021 ◽  
Vol 22 (3) ◽  
pp. 1405
Author(s):  
Emmanuel Panteris ◽  
Anna Kouskouveli ◽  
Dimitris Pappas ◽  
Ioannis-Dimosthenis S. Adamakis
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Wall ◽  
Cell Walls ◽  
Higher Plants ◽  
Cell Plate ◽  
Wild Type ◽  
Microtubule Organization ◽  
Root Cells ◽  
Wall Formation ◽  
In The Wild

Cytokinesis is accomplished in higher plants by the phragmoplast, creating and conducting the cell plate to separate daughter nuclei by a new cell wall. The microtubule-severing enzyme p60-katanin plays an important role in the centrifugal expansion and timely disappearance of phragmoplast microtubules. Consequently, aberrant structure and delayed expansion rate of the phragmoplast have been reported to occur in p60-katanin mutants. Here, the consequences of p60-katanin malfunction in cell plate/daughter wall formation were investigated by transmission electron microscopy (TEM), in root cells of the fra2 Arabidopsis thaliana loss-of-function mutant. In addition, deviations in the chemical composition of cell plate/new cell wall were identified by immunolabeling and confocal microscopy. It was found that, apart from defective phragmoplast microtubule organization, cell plates/new cell walls also appeared faulty in structure, being unevenly thick and perforated by large gaps. In addition, demethylesterified homogalacturonans were prematurely present in fra2 cell plates, while callose content was significantly lower than in the wild type. Furthermore, KNOLLE syntaxin disappeared from newly formed cell walls in fra2 earlier than in the wild type. Taken together, these observations indicate that delayed cytokinesis, due to faulty phragmoplast organization and expansion, results in a loss of synchronization between cell plate growth and its chemical maturation.

A gamma-tubulin-related protein associated with the microtubule arrays of higher plants in a cell cycle-dependent manner

1993 ◽  
Vol 104 (4) ◽  
pp. 1217-1228 ◽  
Author(s):  
B. Liu ◽  
J. Marc ◽  
H.C. Joshi ◽  
B.A. Palevitz
Keyword(s):  
Cell Cycle ◽  
Mammalian Cells ◽  
Higher Plants ◽  
Cortical Microtubule ◽  
Metaphase Plate ◽  
Preprophase Band ◽  
Cell Plate ◽  
Dependent Manner ◽  
Gamma Tubulin ◽  
Cycle Dependent

An antibody specific for a conserved gamma-tubulin peptide identifies a plant polypeptide of 58 kDa. gamma-Tubulin antibody affinity purified from this polypeptide recognizes the centrosome in mammalian cells. Using immunofluorescence microscopy, we determined the distribution of this gamma-tubulin-related polypeptide during the complex changes in microtubule arrays that occur throughout the plant cell cycle. We report a punctate association of gamma-tubulin-related polypeptide with the cortical microtubule array and the preprophase band. As cells enter prophase, gamma-tubulin-related polypeptide accumulates around the nucleus and forms a polar cap from which early spindle microtubules radiate. During metaphase and anaphase, gamma-tubulin-related polypeptide preferentially associates with kinetochore fibers and eventually accumulates at the poles. In telophase, localization occurs over the phragmoplast. gamma-Tubulin-related polypeptide appears to be excluded from the plus ends of microtubules at the metaphase plate and cell plate. Its distribution during the cell cycle may be significant in light of differences in the behavior and organization of plant microtubules. The identification of gamma-tubulin-related polypeptide could help characterize microtubule organizing centers in these organisms.

Relationship between preprophase band organization, F-actin and the division site in Allium

1990 ◽  
Vol 97 (2) ◽  
pp. 283-295
Author(s):  
Y. MINEYUKI ◽  
B. A. PALEVITZ
Keyword(s):  
Higher Plants ◽  
Preprophase Band ◽  
Cell Plate ◽  
Mitotic Apparatus ◽  
Division Plane ◽  
Division Site ◽  
Dividing Cells ◽  
G2 Phase

The preprophase band (PPB) of microtubules (Mts), which appears in the G2 phase of the cell cycle in higher plants but disappears well before the end of karyokinesis, is implicated in the determination of the division plane because its location marks the site at which the phragmoplast/cell plate will fuse with the parental plasmalemma during cytokinesis. The PPB first appears as a rather wide array, which progressively narrows before or during prophase. Actin-containing microfilaments (Mfs) have recently been reported in the PPB, but the role of these elements in PPB organization and/or function remains unclear. The present study employed fluorescence and pharmacological methods in symmetrically and asymmetrically dividing epidermal cells of Allium to probe this problem. Our results show that PPBs in cells treated with 2–200μM cytochalasin D (CD) are still transversely aligned but remain two to three times wider than mature bands in control cells. Treatment for 0.5 h at 20 μM is sufficient to make the PPBs abnormally widel Premitotic nuclear migration in asymmetrically dividing cells is also inhibited by CD, as is the positioning of the mitotic apparatus and the new cell plate. The plate is still transverse, however. Band-like arrays of cortical Mfs become evident in most interphase cells by prophase. The band remains quite wide compared to the final dimensions of the Mt PPB, and clearly encompasses it. Levels of CD as high as 200μM decrease the number of cells with transverse actin bands, although a majority still retain them. Other F-actin arrays are disrupted by the drug. Thus, while CD does not inhibit the formation of an initial, broad, transverse PPB in most cells, it does prevent the narrowing process that defines the precise division site. The role of actin in this effect is discussed.

Microtubule and F-actin dynamics at the division site in living Tradescantia stamen hair cells

1992 ◽  
Vol 103 (4) ◽  
pp. 977-988 ◽  
Author(s):  
A.L. Cleary ◽  
B.E.S. Gunning ◽  
G.O. Wasteneys ◽  
P.K. Hepler
Keyword(s):  
Hair Cells ◽  
Preprophase Band ◽  
Cell Plate ◽  
Laser Scanning Microscopy ◽  
Actin Dynamics ◽  
Cell Cortex ◽  
Living Plant ◽  
Rhodamine Phalloidin ◽  
Division Site ◽  
The Future

We have visualised F-actin and microtubules in living Tradescantia virginiana stamen hair cells by confocal laser scanning microscopy after microinjecting rhodamine-phalloidin or carboxyfluorescein-labelled brain tubulin. We monitored these components of the cytoskeleton as the cells prepared for division at preprophase and progressed through mitosis to cytokinesis. Reorganisation of the interphase cortical cytoskeleton results in preprophase bands of both F-actin and microtubules that coexist in the cell cortex, centred on the site at which the future cell plate will fuse with the parent cell wall. The preprophase band of microtubules is formed from microtubules that polymerise and incorporate tubulin during prophase. The preprophase band of actin may form either by reorganisation of pre-existing filaments or by de novo polymerisation. Both cytoskeletal components disappear from the future division site approximately five minutes prior to the breakdown of the nuclear envelope. Cortical microtubules are undetectable throughout mitosis and cytokinesis, whereas cortical F-actin remains abundant, although it is notably excluded from the division site. The phragmoplast, containing both F-actin and microtubules, expands towards the cortical actin exclusion-zone through a region that has no detectable microtubules or F-actin. The phragmoplast comes to rest in the predefined region of the cortex that is devoid of F-actin. It is proposed that cortical F-actin may act as a “negative” template which could position the phragmoplast and cell plate correctly. This is the first in vivo documentation of F- actin dynamics at the division site in living plant cells.

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