Three-dimensional electron microscopy of the internal nucleolus-associated chromatin and of the nucleolar vacuoles during early germination of Sinapis alba

1986 ◽  
Vol 82 (1) ◽  
pp. 53-71
Author(s):  
R. Deltour ◽  
H. Mosen ◽  
R. Bronchart
Keyword(s):  
Cross Section ◽  
Three Dimensional ◽  
Sinapis Alba ◽  
Serial Sections ◽  
Dense Fibrillar Component ◽  
Dimensional Electron ◽  
Early Germination ◽  
Fibrillar Component ◽  
Dense Chromatin ◽  
Nucleolar Vacuole

Spatial relationships between the internal nucleolus-associated chromatin (NAC) and the numerous nucleolar vacuoles that appear during early germination have been studied in nucleoli of quiescent (non-germinated) and early germinating embryos of Sinapis using serial sections. In quiescent non-vacuolated nucleoli, the transcriptionally inactive internal NAC is a short strand about 900 nm thick that in cross-section appears as heterogeneous fibrillar centres (FCs). At 4 and 6 h after germination one or several large networks of interconnected nucleolar vacuoles develop around the dispersing internal NAC. Clumps of dense chromatin are still present within the nucleolar vacuoles and are probably unfolding into deoxyribonucleoprotein (DNP) fibres (about 110 nm thick), which rapidly intrude within the nucleolar body and form thin chromatin threads. At 24 h after germination the internal NAC is more dispersed and forms, for its greatest part, a long thread (about 240 nm in diameter) wrapped up with a few dense fibrillar component, the whole forming the first outline of a nucleolonema. In cross-section most of the internal NAC appears as homogeneous FCs but short portions remain more condensed and appear as heterogeneous FCs always associated with a nucleolar vacuole. From 48 h the internal NAC is a longer thinner strand (about 160 nm in diameter), probably continuous and surrounded entirely by a homogeneous muff of dense fibrillar component, the whole forming a typical nucleolonema (about 950 nm thick) meandering throughout the nucleolus. Small amounts of the internal NAC still remain undispersed in the form of heterogeneous FCs associated with a nucleolar vacuole. The repeated association of nucleolar vacuoles and dispersing internal NAC suggests that they could play a role in chromatin dispersion and, or, activation by creating a favourable microenvironment.

Three-dimensional localisation of DNA in the nucleolus of Spirogyra by correlated optical tomography and serial ultra-thin sectioning

1990 ◽  
Vol 95 (3) ◽  
pp. 343-352
Author(s):  
E.G. Jordan ◽  
D.J. Rawlins
Keyword(s):  
Optical Microscopy ◽  
Optical Tomography ◽  
Three Dimensional ◽  
Dense Fibrillar Component ◽  
Fibrillar Centre ◽  
Thin Sectioning ◽  
Distribution Matching ◽  
Fibrillar Component ◽  
Dapi Fluorescence

Spirogyra nucleoli were shown by three-dimensional optical microscopy of DAPI fluorescence to contain DNA with a pattern and distribution matching those of the fibrillar centres. This was confirmed using different species with nucleoli showing different sizes of fibrillar centre. Much lower levels of fluorescence were seen corresponding to the dense fibrillar component. Nearly all the DAPI fluorescence arises from the fibrillar centres or from regions very close to their surface, indicating that this is the site of nucleolar transcription.

Electron Momentum Densities by (y, e y)-Spectroscopy

1993 ◽  
Vol 48 (1-2) ◽  
pp. 273-278
Author(s):  
F. Bell ◽  
Th. Tschentscher ◽  
A. J. Rollason
Keyword(s):  
Cross Section ◽  
Three Dimensional ◽  
Momentum Density ◽  
Scattered Photon ◽  
Doppler Broadening ◽  
Dimensional Electron ◽  
Electron Momentum ◽  
Triple Differential Cross Section ◽  
Photon Intensity ◽  
Coincidence Measurements

Abstract We report coincidence measurements of inelastically scattered 148 keV photons and the recoil electrons generated within thin Al and Cu foils. We have analyzed both the Doppler broadening of the scattered photon intensity and the angular correlation of the recoil electron. This corresponds to different scans through the three-dimensional electron momentum density of the target. Estimates of the triple-differential cross-section for Compton scattering are given. The experiment was performed with synchrotron radiation from a bending magnet of the DORIS storage ring at DESY, Hamburg.

Three-Dimensional Reconstruction Using Stereo Pairs from Serial Thick Sections

1977 ◽  
Vol 35 ◽  
pp. 562-563
Author(s):  
Robert Glaeser ◽  
Thomas Bauer ◽  
David Grano
Keyword(s):  
Three Dimensional ◽  
Dimensional Structure ◽  
Serial Sections ◽  
Thin Sections ◽  
3 Dimensional ◽  
Transmission Electron ◽  
Freeze Dried ◽  
Dimensional Reconstruction ◽  
Stereo Imagery ◽  
Whole Mounts

In transmission electron microscopy, the 3-dimensional structure of an object is usually obtained in one of two ways. For objects which can be included in one specimen, as for example with elements included in freeze- dried whole mounts and examined with a high voltage microscope, stereo pairs can be obtained which exhibit the 3-D structure of the element. For objects which can not be included in one specimen, the 3-D shape is obtained by reconstruction from serial sections. However, without stereo imagery, only detail which remains constant within the thickness of the section can be used in the reconstruction; consequently, the choice is between a low resolution reconstruction using a few thick sections and a better resolution reconstruction using many thin sections, generally a tedious chore. This paper describes an approach to 3-D reconstruction which uses stereo images of serial thick sections to reconstruct an object including detail which changes within the depth of an individual thick section.

Correlated Studies of Cellular Interactions Using TEM, Freeze Etching, and SEM

1974 ◽  
Vol 32 ◽  
pp. 320-321
Author(s):  
J. P. Revel
Keyword(s):  
Developmental Stages ◽  
Three Dimensional ◽  
Cell Movement ◽  
Cellular Interactions ◽  
Serial Sections ◽  
Cell Sheets ◽  
Freeze Etching ◽  
Early Developmental

Movement of individual cells or of cell sheets and complex patterns of folding play a prominent role in the early developmental stages of the embryo. Our understanding of these processes is based on three- dimensional reconstructions laboriously prepared from serial sections, and from autoradiographic and other studies. Many concepts have also evolved from extrapolation of investigations of cell movement carried out in vitro. The scanning electron microscope now allows us to examine some of these events in situ. It is possible to prepare dissections of embryos and even of tissues of adult animals which reveal existing relationships between various structures more readily than used to be possible vithout an SEM.

Application of stereological analysis of cell volume to isolated myocytes in culture with and without adrenergic innervation

1990 ◽  
Vol 48 (3) ◽  
pp. 416-417
Author(s):  
Jane K. Rosenthal ◽  
Dianne L. Atkins ◽  
William J. Marvin ◽  
Penny A. Krumm
Keyword(s):  
Cardiac Myocytes ◽  
Structural Changes ◽  
Three Dimensional ◽  
Adrenergic Innervation ◽  
Culture Cell ◽  
Serial Sections ◽  
Newborn Rats ◽  
Primary Culture Cell ◽  
Biological Studies ◽  
Cell Volumes

To comprehend structural changes in cardiac myocytes accompanying adrenergic innervation, it is essential that a three dimensional analysis be performed. To date, biological studies which utilize stereological methods have been limited to cells in tissue and in organs. Our laboratory has utilized current stereological techniques for measuring absolute volumes of individual myocytes in primary culture. Cell volumes are calculated for two distinct groups of cells at 96 hours in culture: isolated myocytes and myocytes innervated with adrenergic neurons (Figure 1).Cardiac myocytes are cultured from the ventricular apices of newborn rats. Cells are plated directly onto tissue culture dishes with or without preplated explants from the paravertebral thoracolumbar sympathetic chain. On day four cultures are photographed and marked for one-to-one cell location. Following conventional fixation and embeddment in eponate-12, the cells are relocated and mounted for microtomy. The cells are completely sectioned at 120nm in their parallel orientation to the surface of the dish (Figure 2). Serial sections are collected on formvar coated slotted grids and are recorded in sequence.

SAF file: It's really three dimensional file

2018 ◽  
Vol 14 (1) ◽  
pp. 1
Author(s):  
Prof. Dr. Jamal Aziz Mehdi
Keyword(s):  
Cross Section ◽  
Root Canal ◽  
Three Dimensional ◽  
Circular Cross Section ◽  
Circular Motion ◽  
Root Canal Treatment ◽  
Metal Core ◽  
Rotating Blade

The biological objectives of root canal treatment have not changed over the recentdecades, but the methods to attain these goals have been greatly modified. Theintroduction of NiTi rotary files represents a major leap in the development ofendodontic instruments, with a wide variety of sophisticated instruments presentlyavailable (1, 2).Whatever their modification or improvement, all of these instruments have onething in common: they consist of a metal core with some type of rotating blade thatmachines the canal with a circular motion using flutes to carry the dentin chips anddebris coronally. Consequently, all rotary NiTi files will machine the root canal to acylindrical bore with a circular cross-section if the clinician applies them in a strictboring manner

ANALYSIS OF THE GENERAL EQUATIONS OF THE TRANSVERSE VIBRATION OF A PIECEWISE UNIFORM VISCOELASTIC PLATE

2020 ◽  
Vol 7 (3) ◽  
pp. 52-56
Author(s):  
MMATMATISA JALILOV ◽  
◽  
RUSTAM RAKHIMOV ◽  
Keyword(s):  
Cross Section ◽  
Transverse Vibration ◽  
Three Dimensional ◽  
Viscoelastic Plate ◽  
Constant Thickness ◽  
Regional Problem ◽  
Rigid Contact ◽  
Under Construction ◽  
Derivatives Of ◽  
Theoretical Results

This article discusses the analysis of the general equations of the transverse vibration of a piecewise homogeneous viscoelastic plate obtained in the “Oscillation of inlayer plates of constant thickness” [1]. In the present work on the basis of a mathematical method, the approached theory of fluctuation of the two-layer plates, based on plate consideration as three dimensional body, on exact statement of a three dimensional mathematical regional problem of fluctuation is stood at the external efforts causing cross-section fluctuations. The general equations of fluctuations of piecewise homogeneous viscoelastic plates of the constant thickness, described in work [1], are difficult on structure and contain derivatives of any order on coordinates x, y and time t and consequently are not suitable for the decision of applied problems and carrying out of engineering calculations. For the decision of applied problems instead of the general equations it is expedient to use confidants who include this or that final order on derivatives. The classical equations of cross-section fluctuation of a plate contain derivatives not above 4th order, and for piecewise homogeneous or two-layer plates the elementary approached equation of fluctuation is the equation of the sixth order. On the basis of the analytical decision of a problem the general and approached decisions of a problem are under construction, are deduced the equation of fluctuation of piecewise homogeneous two-layer plates taking into account rigid contact on border between layers, and also taking into account mechanical and rheological properties of a material of a plate. The received theoretical results for the decision of dynamic problems of cross-section fluctuation of piecewise homogeneous two-layer plates of a constant thickness taking into account viscous properties of their material allow to count more precisely the is intense-deformed status of plates at non-stationary external loadings.

Boundary element method in numerical study of three-dimensional Stokes flows in channels of arbitrary shape

2012 ◽  
Vol 9 (1) ◽  
pp. 94-97
Author(s):  
Yu.A. Itkulova
Keyword(s):  
Boundary Element Method ◽  
Boundary Element ◽  
Cross Section ◽  
Numerical Study ◽  
Three Dimensional ◽  
Cylindrical Tube ◽  
Variable Cross Section ◽  
Variable Cross ◽  
Periodic Flows ◽  
Element Method

In the present work creeping three-dimensional flows of a viscous liquid in a cylindrical tube and a channel of variable cross-section are studied. A qualitative triangulation of the surface of a cylindrical tube, a smoothed and experimental channel of a variable cross section is constructed. The problem is solved numerically using boundary element method in several modifications for a periodic and non-periodic flows. The obtained numerical results are compared with the analytical solution for the Poiseuille flow.

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