Surface Features of Smooth Muscle Cells from the Mesenteric Artery and Vas Deferens

1971 ◽  
Vol 8 (2) ◽  
pp. 427-443 ◽  
Author(s):  
C. E. DEVINE ◽  
F. O. SIMPSON ◽  
W. S. BERTAUD
Keyword(s):  
Smooth Muscle ◽  
Sarcoplasmic Reticulum ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Mesenteric Artery ◽  
Vas Deferens ◽  
Muscle Cells ◽  
Mesenteric Arteries ◽  
Dense Bodies ◽  
Freeze Etching

Smooth muscle cells of small mesenteric arteries and vas deferens of guinea-pig were examined by freeze-etching. The most striking finding was that the surface vesicles lie in roughly longitudinal rows, with areas of membrane free of vesicles in between. The areas free of vesicles are believed to correspond to areas occupied by dense bodies in conventionally fixed and sectioned material. Other cell constituents which could be identified included sarcoplasmic reticulum and, probably, thick myofilaments.

Freeze-Etch Studies on the Innervation of Mesenteric Arteries and Vas Deferens

1971 ◽  
Vol 9 (2) ◽  
pp. 411-425
Author(s):  
C. E. DEVINE ◽  
F. O. SIMPSON ◽  
W. S. BERTAUD
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Schwann Cells ◽  
Synaptic Vesicles ◽  
Vas Deferens ◽  
Muscle Cells ◽  
Mesenteric Arteries ◽  
Axonal Membrane ◽  
Freeze Etching

The innervation of mesenteric arteries and vas deferens of guinea-pig and vas deferens of mouse was examined by freeze-etching. Axons in bundles at large distances from the smooth muscle cells, were invested by Schwann cells and contained mainly neurotubules, while axons close to the smooth muscle cells had varicosities up to 1.6 µm in diameter and 2.0 µm long containing mainly small (approximately 50 nm) and large (approximately 100 nm) synaptic vesicles. Vascular axons differed from those in the vas deferens in that the former were at the medial adventitial border with an observed closest neuromuscular distance of approximately 200 nm and the latter were between smooth muscle cells at distances of 20-50 nm. Depressions of the axonal surface were seen and particles up to 15 nm were found on the axonal membrane.

scholarly journals Local Ca2+transients and distribution of BK channels and ryanodine receptors in smooth muscle cells of guinea-pig vas deferens and urinary bladder

2001 ◽  
Vol 534 (2) ◽  
pp. 313-326 ◽  
Author(s):  
Yoshiaki Ohi ◽  
Hisao Yamamura ◽  
Norihiro Nagano ◽  
Susumu Ohya ◽  
Katsuhiko Muraki ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Vas Deferens ◽  
Ryanodine Receptors ◽  
Muscle Cells ◽  
Bk Channels

Expression of voltage-dependent K+ channel genes in mesenteric artery smooth muscle cells

1999 ◽  
Vol 277 (5) ◽  
pp. G1055-G1063 ◽  
Author(s):  
Chuanli Xu ◽  
Yanjie Lu ◽  
Guanghua Tang ◽  
Rui Wang
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Mesenteric Artery ◽  
Muscle Cells ◽  
Mesenteric Arteries ◽  
Delayed Rectifier ◽  
Kv Channel ◽  
Channel Proteins ◽  
Voltage Dependent ◽  
Encoding Genes

Molecular basis of native voltage-dependent K+(Kv) channels in smooth muscle cells (SMCs) from rat mesenteric arteries was investigated. The whole cell patch-clamp study revealed that a 4-aminopyridine-sensitive delayed rectifier K+ current ( I K) was the predominant K+ conductance in these cells. A systematic screening of the expression of 18 Kv channel genes using RT-PCR technique showed that six I K-encoding genes (Kv1.2, Kv1.3, Kv1.5, Kv2.1, Kv2.2, and Kv3.2) were expressed in mesenteric artery. Although no transient outward Kv currents ( I A) were recorded in the studied SMCs, transcripts of multiple I A-encoding genes, including Kv1.4, Kv3.3, Kv3.4, Kv4.1, Kv4.2, and Kv4.3 as well as I A-facilitating Kv β-subunits (Kvβ1, Kvβ2, and Kvβ3), were detected in mesenteric arteries. Western blot analysis demonstrated that four I K-related Kv channel proteins (Kv1.2, Kv1.3, Kv1.5, and Kv2.1) were detected in mesenteric artery tissues. The presence of Kv1.2, Kv1.3, Kv1.5, and Kv2.1 channel proteins in isolated SMCs was further confirmed by immunocytochemistry study. Our results suggest that the native I K in rat mesenteric artery SMCs might be generated by heteromultimerization of Kv genes.

scholarly journals The Effect of Intracellular Current Pulses in Smooth Muscle Cells of the Guinea Pig Vas Deferens at Rest and during Transmission

1967 ◽  
Vol 50 (10) ◽  
pp. 2459-2475 ◽  
Author(s):  
M. R. Bennett
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Action Potential ◽  
Smooth Muscle Cells ◽  
Vas Deferens ◽  
Electrical Coupling ◽  
Muscle Cells ◽  
Active Response ◽  
Current Pulses ◽  
Passive Response

The effect of intracellular current pulses on the membrane of smooth muscle cells of the guinea pig vas deferens at rest and during transmission was studied. Two main response types were identified: active response cells, in which a spike was initiated in response to depolarizing currents of sufficient strength and duration; passive response cells, in which depolarizing currents gave only electrotonic potential changes. These cells were three times more numerous than the active response cells. During the crest of the active response the input resistance fell by about 25% of the resting value. Comparison of the active response with the action potential due to stimulating the hypogastric nerve showed that the former was smaller in amplitude and had a slower rate of rise and higher threshold. Electrical coupling occurred between the smooth muscle cells during the propagation of the action potential. Depolarizing current pulses had no effect on the amplitude of the excitatory junction potential (E.J.P.) in passive response cells, but in general did decrease its amplitude in active response cells. These results are discussed with respect to the mechanism of autonomic neuroeffector transmission.

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