scholarly journals The effects of fibronectin on the migration of human foreskin fibroblasts and Syrian hamster melanoma cells into three-dimensional gels of native collagen fibres

1981 ◽  
Vol 48 (1) ◽  
pp. 301-314
Author(s):  
S.L. Schor ◽  
A.M. Schor ◽  
G.W. Bazill
Keyword(s):  
Human Skin ◽  
Syrian Hamster ◽  
Melanoma Cells ◽  
Skin Fibroblasts ◽  
Collagen Gels ◽  
Human Skin Fibroblasts ◽  
Collagen Fibres ◽  
3 Dimensional ◽  
Native Collagen ◽  
Hamster Melanoma

The effects of fibronectin on the migration of human skin fibroblasts and Syrian hamster melanoma cells into 3-dimensional gels of native collagen fibres have been examined. Cell migration into the 3-dimensional gel was measured by plating cells on the gel surface and then determining the percentage of cells within the gel at various times thereafter by direct microscopic examination. We find that fibronectin bound to collagen inhibits the migration of human skin fibroblasts and stimulates the migration of melanoma cells into the gel matrix. Fibronectin had no apparent effect on cell adhesion to the collagen gels, proliferation or morphology under the conditions studied.

scholarly journals Specific association of iduronic acid-rich dermatan sulphate with the extracellular matrix of human skin fibroblasts cultured on collagen gels

1983 ◽  
Vol 215 (1) ◽  
pp. 107-116 ◽  
Author(s):  
J T Gallagher ◽  
N Gasiunas ◽  
S L Schor
Keyword(s):  
Human Skin ◽  
Heparan Sulphate ◽  
Surface Membrane ◽  
Skin Fibroblasts ◽  
Collagen Gels ◽  
Human Skin Fibroblasts ◽  
Dermatan Sulphate ◽  
Cellular Functions ◽  
Iduronic Acid ◽  
Sulphated Glycosaminoglycans

Human skin fibroblasts cultured on collagen gels produced two dermatan sulphate species, one, enriched in iduronic acid residues, that bound specifically to the collagenous fibres of the gel, the other, enriched in glucuronic acid, that accumulated in the culture medium. Collagen-binding and collagen-non-binding dermatan sulphates were also produced by cells grown on plastic surfaces, but in these cultures each constituent was released into the growth medium. Net synthesis of dermatan sulphate was 3-fold higher in cells maintained on collagen gels. In contrast, heparan sulphate synthesis was not influenced by the nature of the culture surface. The concentration of heparan sulphate in surface-membrane extracts was similar for cells grown on plastic and on collagen gels, but cells cultured on collagen showed a notable increase in the content of surface-membrane dermatan sulphate. The patterns of synthesis and distribution of sulphated glycosaminoglycans observed in skin fibroblasts maintained on collagen gels may reflect differentiated cellular functions.

scholarly journals Studies on the mechanism of hydrated collagen gel reorganization by human skin fibroblasts

1985 ◽  
Vol 79 (1) ◽  
pp. 67-81 ◽  
Author(s):  
C. Guidry ◽  
F. Grinnell
Keyword(s):  
Human Skin ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Collagen Fibrils ◽  
Skin Fibroblasts ◽  
Electron Microscopic Level ◽  
Total Protein Content ◽  
Collagen Gels ◽  
Human Skin Fibroblasts ◽  
Electron Microscopic

During reorganization of collagen gels by human skin fibroblasts the total protein content of the gels remained approximately constant. Only 5% of the collagen was degraded, although the volume of the gels decreased by 85% or more. It could be concluded, therefore, that gel reorganization required physical rearrangement of pre-existing collagen fibrils rather than degradation of the original collagen and resynthesis of a new matrix. Collagen molecules in the gels were not covalently crosslinked or otherwise modified enzymically during gel reorganization, as determined by sodium dodecyl sulphate/polyacrylamide gel electrophoresis and collagen repolymerization studies. Serum was required for gel reorganization and, in the absence of serum, cell spreading was predominantly filipodial, i.e. there was little cytoplasmic reorganization. At the electron-microscopic level it was found that many more collagen fibrils became associated with the cells in the presence of serum than in its absence. Serum was also found to promote the synthesis and secretion of proteins by the cells, and conditioned medium could take the place of serum in promoting gel reorganization. The involvement of cell-secreted factors was also demonstrated by the ability of cycloheximide to inhibit gel reorganization. Finally, when gel reorganization was stopped by adding cytochalasin D to the incubations or removing cells by detergent treatment, a small but significant re-expansion of the collagen fibrils was observed. Consequently, a portion of the collagen that had been physically reorganized by the gels was unstable and could not hold its position without continued force exerted by the cells.

Oxidative stress impairs the repair of oxidative DNA base modifications in human skin fibroblasts and melanoma cells

DNA Repair ◽  
2008 ◽  
Vol 7 (6) ◽  
pp. 912-921 ◽  
Author(s):  
Wolfgang Eiberger ◽  
Beate Volkmer ◽  
Rachel Amouroux ◽  
Claudine Dhérin ◽  
J. Pablo Radicella ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Human Skin ◽  
Melanoma Cells ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Dna Base ◽  
Base Modifications

117-LB: Nonviral Direct Reprogramming of Human Skin Fibroblasts into Hormone-Expressing ß-Like Cell Clusters

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 117-LB
Author(s):  
LUKE R. LEMMERMAN ◽  
MARIA ANGELICA RINCON-BENAVIDES ◽  
SARAH A. TERSEY ◽  
BRITANI N. BLACKSTONE ◽  
HEATHER M. POWELL ◽  
...  
Keyword(s):  
Human Skin ◽  
Skin Fibroblasts ◽  
Direct Reprogramming ◽  
Human Skin Fibroblasts ◽  
Cell Clusters

Protective Effects of Green Tea Seed Extract against UVB-irradiated Human Skin Fibroblasts

2014 ◽  
Vol 43 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Ok Kyung Kim ◽  
Da-Eun Nam ◽  
Min-Jae Lee ◽  
Namgil Kang ◽  
Jae-Youn Lim ◽  
...  
Keyword(s):  
Human Skin ◽  
Green Tea ◽  
Seed Extract ◽  
Skin Fibroblasts ◽  
Protective Effects ◽  
Human Skin Fibroblasts

The intralysosomal pH in cultured human skin fibroblasts in relation to cystine accumulation in patients with cystinosis

1983 ◽  
Vol 116 (1) ◽  
pp. 154-161 ◽  
Author(s):  
Ronald P.J. Oude Elferink ◽  
Erik Harms ◽  
Anneke Strijland ◽  
Joseph M. Tager
Keyword(s):  
Human Skin ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts

The Effect of Practolol, In Vitro Generated Metabolites of Practolol and Chemical Analogues on the Rate of Growth of Human Skin Fibroblasts

1984 ◽  
Vol 12 (2) ◽  
pp. 89-97
Author(s):  
Graham R. Elliott ◽  
H.E. Amos ◽  
James W. Bridges
Keyword(s):  
Human Skin ◽  
Psoriasis Patient ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Cell Type ◽  
Normal Human Skin ◽  
Rate Of Growth ◽  
Structural Analogues ◽  
Normal Human

The rate of growth of normal human skin fibroblasts was inhibited in a dose related, reversible, fashion by practolol (N-4-(2-hydroxy)-3 (1-methyl)-aminopropoxyphenylacetamine) (ID50 1.35 ± 0.14 x 10-3M), propranolol (1-(isopropylamino)-3(1-naphthyl-oxy)-2-propranolol) (ID50 0.145 ± 0.02 x 10-3M) and paracetamol (N-(4-hydroxyphenyl) acetamide) (ID50 0.85 ± 0.2 x 10-3M). Skin fibroblasts isolated from a psoriasis patient were more sensitive towards practolol (ID50 0.48 ± 0.14 x 10-3M) and propranolol (ID50 0.032 ± 0.002 x 10-3M), but less sensitive towards paracetamol (ID50 1.3 ± 0.07 x 10-3M). In vitro generated metabolites of practolol, using normal or Arochlor 1254-pretreated hamster liver preparations, and structural analogues of practolol had no effect upon the growth of either cell type.

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