Effects on cell adhesion and membrane fluidity of changes in plasmalemmal lipids in mouse L929 cells

1977 ◽  
Vol 26 (1) ◽  
pp. 47-55
Author(s):  
BE Schaeffer ◽  
AS Curtis
Keyword(s):  
Cell Adhesion ◽  
Membrane Fluidity ◽  
L929 Cells

scholarly journals A correlation between membrane fluidity and the critical temperature for cell adhesion.

1976 ◽  
Vol 71 (2) ◽  
pp. 670-674 ◽  
Author(s):  
M J Ueda ◽  
T Ito ◽  
T S Okada ◽  
S I Ohnishi
Keyword(s):  
Cell Adhesion ◽  
Critical Temperature ◽  
Stearic Acid ◽  
Membrane Fluidity ◽  
Characteristic Temperatures ◽  
Esr Spectrum ◽  
Plastic Dish

BHK 21 cells can adhere to a protein-coated plastic dish in the presence of Ca2+ at temperatures above 12 degrees C. However, they cannot adhere below 8 degrees C. The ESR spectrum of cells spin-labeled with a stearic acid label indicated that the membrane fluidity changed characteristically at 10 degrees C, 20 degrees C, and 30 degrees C. The critical temperature for cell adhesion coincided well with one of the characteristic temperatures for the membrane fluidity change. In the case of adhesion in the presence of Mg2+, no such correlation was observed.

scholarly journals Clusters of neural cell adhesion molecule at sites of cell-cell contact.

1992 ◽  
Vol 116 (2) ◽  
pp. 449-463 ◽  
Author(s):  
R J Bloch
Keyword(s):  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Neural Cell Adhesion Molecule ◽  
Neural Cell ◽  
Extracellular Proteins ◽  
Cell Contact ◽  
L929 Cells ◽  
Neural Cell Adhesion ◽  
Cell Cell

I have examined the distribution of neural cell adhesion molecule (N-CAM) in cultured C2 myogenic cells and other cell lines to determine if N-CAM accumulates at sites of cell-cell contact. C2 cells growing in log phase display large clusters of neural cell adhesion molecule where they contact each other. These clusters are remarkably stable, do not form at cell-substrate contacts, and appear not to be enriched in a number of other cytoskeletal, membrane, or extracellular proteins. Thus, N-CAM clusters form preferentially in response to cell-cell contact and are specifically enriched in N-CAM. As C2 cultures mature and differentiate, clusters persist at contacts between aligning myoblasts and between myotubes, consistent with a role in myogenesis. N-CAM is also enriched at cell-cell contacts in cultures of PC12, NRK, and CHO cells. These cells have significant amounts of N-CAM as detected on immunoblots. Clusters are not seen in L929 cells, which do not have detectable amounts of N-CAM. Coculture of these cells with C2 cells results in the clustering of N-CAM at heterologous contacts between C2 cells and NRK, CHO, or PC12 cells, but not between C2 cells and L929 cells. These results suggest that N-CAM specifically accumulates where N-CAM-bearing cells contact one another. Clustering of N-CAM may be an important step in strengthening intercellular adhesion.

Membrane fluidity aspects in endocytosis; a study with the fluorescent probe trimethylamino-diphenylhexatriene in L929 cells

1991 ◽  
Vol 71 (3) ◽  
pp. 293-296 ◽  
Author(s):  
Dominique Illinger ◽  
Philippe Poindron ◽  
Jean-Georges Kuhry
Keyword(s):  
Membrane Fluidity ◽  
Fluorescent Probe ◽  
L929 Cells

Membrane fluidity and lipid content of human spermatozoa selected by swim-up method

2001 ◽  
Vol 24 (6) ◽  
pp. 327-334 ◽  
Author(s):  
A. Force ◽  
G. Grizard ◽  
M. N. Giraud ◽  
C. Motta ◽  
B. Sion ◽  
...  
Keyword(s):  
Membrane Fluidity ◽  
Lipid Content ◽  
Human Spermatozoa
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