scholarly journals Ana1/Cep295 helps recruit Polo/PLK1 to centrioles to promote mitotic PCM assembly and centriole elongation

2021 ◽  
Author(s):  
Ines Alvarez-Rodrigo ◽  
Alan Wainman ◽  
Saroj Saurya ◽  
Jordan W. Raff
Keyword(s):  
Cell Cycle ◽  
Multiple Functions ◽  
Centriole Duplication ◽  
Polo Kinase ◽  
Cell Cycle Regulator ◽  
Pericentriolar Material

Polo kinase (PLK1) is a master cell cycle regulator that is recruited to various subcellular structures, often by its Polo-Box domain (PBD), which binds to phosphorylated S-pS/pT motifs. Polo/PLK1 has multiple functions at centrioles and centrosomes, and we previously showed that in Drosophila phosphorylated Sas-4 initiates Polo/PLK1 recruitment to newly formed centrioles, while phosphorylated Spd-2 recruits Polo/PLK1 to the Pericentriolar Material (PCM) that assembles around mother centrioles in mitosis. Here, we show that Ana1 (Cep295 in humans) also helps to recruit Polo to mother centrioles in Drosophila. If Ana1-dependent Polo/PLK1 recruitment is impaired, mother centrioles can still duplicate, disengage from their daughters and form functional cilia, but they can no longer efficiently assemble mitotic PCM or elongate during G2. We conclude that Ana1 helps recruit Polo/PLK1 to mother centrioles to specifically promote mitotic centrosome assembly and centriole elongation in G2, but not centriole duplication, centriole disengagement or cilia assembly.

scholarly journals Ana1 recruits PLK1 to mother centrioles to promote mitotic PCM assembly and centriole elongation

2020 ◽  
Author(s):  
Ines Alvarez-Rodrigo ◽  
Alan Wainman ◽  
Jordan W. Raff
Keyword(s):  
Cell Cycle ◽  
Multiple Functions ◽  
Centriole Duplication ◽  
Polo Kinase ◽  
Cell Cycle Regulator ◽  
Pericentriolar Material ◽  
Phosphorylation Cascade

AbstractPolo kinase (PLK1) is a master cell cycle regulator that is recruited to various subcellular structures by its Polo-Box domain (PBD), which binds to phosphorylated S-pS/pT motifs. Polo has multiple functions at centrioles and centrosomes, and we previously showed that phosphorylated Sas-4 initiates Polo recruitment to newly formed centrioles, while phosphorylated Spd-2 recruits Polo to the mitotic Pericentriolar Material (PCM) that assembles around mother centrioles. Here, we investigate whether additional proteins recruit Polo to centrioles and/or centrosomes, and find that Ana1 (Cep295 in mammals) helps recruit Polo to mother centrioles. If this function is impaired, mother centrioles can still duplicate and disengage from their daughters, but they can no longer efficiently assemble a mitotic PCM or elongate their centrioles in G2. Thus, Ana1 is part of a sequential phosphorylation cascade that recruits Polo to centrioles to drive mitotic centrosome assembly and centriole elongation in G2, but not centriole duplication or disengagement.

scholarly journals Inhibition of Cdc42 during mitotic exit is required for cytokinesis

2013 ◽  
Vol 202 (2) ◽  
pp. 231-240 ◽  
Author(s):  
Benjamin D. Atkins ◽  
Satoshi Yoshida ◽  
Koji Saito ◽  
Chi-Fang Wu ◽  
Daniel J. Lew ◽  
...  
Keyword(s):  
Cell Cycle ◽  
General Feature ◽  
Budding Yeast ◽  
Mitotic Exit ◽  
Division Site ◽  
Polo Kinase ◽  
Cell Cycle Regulator ◽  
P21 Activated Kinase

The role of Cdc42 and its regulation during cytokinesis is not well understood. Using biochemical and imaging approaches in budding yeast, we demonstrate that Cdc42 activation peaks during the G1/S transition and during anaphase but drops during mitotic exit and cytokinesis. Cdc5/Polo kinase is an important upstream cell cycle regulator that suppresses Cdc42 activity. Failure to down-regulate Cdc42 during mitotic exit impairs the normal localization of key cytokinesis regulators—Iqg1 and Inn1—at the division site, and results in an abnormal septum. The effects of Cdc42 hyperactivation are largely mediated by the Cdc42 effector p21-activated kinase Ste20. Inhibition of Cdc42 and related Rho guanosine triphosphatases may be a general feature of cytokinesis in eukaryotes.

scholarly journals Global cellular response to chemical perturbation of PLK4 activity and abnormal centrosome number

2021 ◽  
Author(s):  
Johnny M Tkach ◽  
Jonathan Strecker ◽  
Daniel Durocher ◽  
Laurence Pelletier
Keyword(s):  
Cell Cycle ◽  
Protein Kinase ◽  
Cellular Response ◽  
Growth Arrest ◽  
Centriole Duplication ◽  
Genome Wide ◽  
Evolutionarily Conserved ◽  
Pericentriolar Material ◽  
Dependent Growth ◽  
Chemical Perturbation

Centrosomes consist of two centrioles surrounded by pericentriolar material and are the main microtubule organizing centre in metazoans. Centrosome number is tightly regulated by limiting centriole duplication to a single round per cell cycle. This control is achieved by multiple mechanisms, including the regulation of the protein kinase PLK4, a master regulator of centrosome biogenesis. In an evolutionarily conserved process, altered centrosome numbers cause a p53-dependent growth arrest through mechanisms that are still poorly defined. To gain insights into this process, we used a series of genome-wide CRISPR/Cas9 screens to identify factors important for growth arrest after chemically altering PLK4 activity to cause too many or too few centrosomes. We identify TRIM37 as a key mediator of growth arrest when PLK4 activity is partially or fully inhibited but is not required for growth arrest triggered by supernumerary centrosomes. Moreover, this activity is independent of its role as an E3 ligase and distinct from other TRIM37 functions described to date. We propose that altered PLK4 activity itself can signal growth arrest.

scholarly journals Centrobin

2005 ◽  
Vol 171 (3) ◽  
pp. 437-445 ◽  
Author(s):  
Chaozhong Zou ◽  
Jun Li ◽  
Yujie Bai ◽  
William T. Gunning ◽  
David E. Wazer ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Mammalian Cells ◽  
Cell Cycle Progression ◽  
S Phase ◽  
Cycle Progression ◽  
Centriole Duplication ◽  
Pericentriolar Material ◽  
Core Components ◽  
Interfering Rna ◽  
Daughter Centriole

In mammalian cells, the centrosome consists of a pair of centrioles and amorphous pericentriolar material. The pair of centrioles, which are the core components of the centrosome, duplicate once per cell cycle. Centrosomes play a pivotal role in orchestrating the formation of the bipolar spindle during mitosis. Recent studies have linked centrosomal activity on centrioles or centriole-associated structures to cytokinesis and cell cycle progression through G1 into the S phase. In this study, we have identified centrobin as a centriole-associated protein that asymmetrically localizes to the daughter centriole. The silencing of centrobin expression by small interfering RNA inhibited centriole duplication and resulted in centrosomes with one or no centriole, demonstrating that centrobin is required for centriole duplication. Furthermore, inhibition of centriole duplication by centrobin depletion led to impaired cytokinesis.

scholarly journals The centriole duplication cycle

2014 ◽  
Vol 369 (1650) ◽  
pp. 20130460 ◽  
Author(s):  
Elif Nur Fırat-Karalar ◽  
Tim Stearns
Keyword(s):  
Cell Cycle ◽  
Cell Polarization ◽  
Brain Diseases ◽  
Basal Bodies ◽  
Animal Cells ◽  
Centriole Duplication ◽  
Pericentriolar Material ◽  
Cilia And Flagella ◽  
Duplication Cycle ◽  
Lower Plant

Centrosomes are the main microtubule-organizing centre of animal cells and are important for many critical cellular and developmental processes from cell polarization to cell division. At the core of the centrosome are centrioles, which recruit pericentriolar material to form the centrosome and act as basal bodies to nucleate formation of cilia and flagella. Defects in centriole structure, function and number are associated with a variety of human diseases, including cancer, brain diseases and ciliopathies. In this review, we discuss recent advances in our understanding of how new centrioles are assembled and how centriole number is controlled. We propose a general model for centriole duplication control in which cooperative binding of duplication factors defines a centriole ‘origin of duplication’ that initiates duplication, and passage through mitosis effects changes that license the centriole for a new round of duplication in the next cell cycle. We also focus on variations on the general theme in which many centrioles are created in a single cell cycle, including the specialized structures associated with these variations, the deuterosome in animal cells and the blepharoplast in lower plant cells.

ZNF403, a Novel Cell Cycle Regulator

2013 ◽  
Vol 40 (2) ◽  
pp. 147
Author(s):  
Rui GUAN ◽  
De-Fu HOU ◽  
Xiang RAO ◽  
Yong-Jun GUAN ◽  
Yong-Mei OUYANG ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Regulator
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