scholarly journals Cell scientists to watch – Zdeněk Lánský and Marcus Braun

2021 ◽  
Vol 134 (11) ◽  
Keyword(s):  
Molecular Motors ◽  
Cell Biology ◽  
Cytoskeletal Proteins ◽  
Force Generation ◽  
Max Planck ◽  
King's College ◽  
Czech Academy ◽  
Molecular Cell Biology ◽  
Set Up ◽  
Academy Of Sciences

ABSTRACT Zdeněk Lánský received his PhD in physics from the Charles University in Prague in 2006. He then moved to The Netherlands for his postdoc, where he studied the biophysics of molecular motors – first at the Vrije Universiteit in Amsterdam with Erwin Peterman, and then at Wageningen University with Marcel Janson. In 2011 he joined the lab of Stefan Diez at the B CUBE Center for Molecular Bioengineering in Dresden, where his research focused on crosslinker-mediated force generation in the cytoskeleton. Zdeněk set up his lab at the Institute of Biotechnology, Czech Academy of Sciences, BIOCEV in 2015, which he has run together with Marcus Braun since 2017. Marcus Braun received his PhD in biochemistry from King's College London in 2009 for his work on microtubule-based molecular motors in the labs of Andrew McAinsh and Rob Cross. He then moved to Dresden for his postdoc to study cytoskeletal transport, with a particular focus on microtubule crosslinkers, in Stefan Diez's lab – first at the Max Planck Institute of Molecular Cell Biology and Genetics, then later at B CUBE. In 2017, he joined Zdeněk Lánský at the Institute of Biotechnology, Czech Academy of Sciences, BIOCEV, as a principal investigator. The Lánský and Braun lab investigates ensemble dynamics of cytoskeletal proteins.

scholarly journals First person – Sarita Hebbar and Malte Lehmann

Biology Open ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. bio058519
Keyword(s):  
Metabolic Pathways ◽  
Cell Biology ◽  
Early Career ◽  
First Person ◽  
Max Planck ◽  
Early Career Researchers ◽  
Splicing Regulator ◽  
Inflammatory Bowel ◽  
Molecular Cell Biology ◽  
Selection Of

ABSTRACTFirst Person is a series of interviews with the first authors of a selection of papers published in Biology Open, helping early-career researchers promote themselves alongside their papers. Sarita Hebbar and Malte Lehmann are co-first authors on ‘Mutations in the splicing regulator Prp31 lead to retinal degeneration in Drosophila’, published in BiO. Sarita is a post-doctoral fellow in the lab of Elisabeth Knust at the Max Planck Institute of Molecular Cell Biology & Genetics, investigating how the same metabolic pathways regulate temporally distinct processes (in morphogenesis and later in tissue homoestasis). Malte is a post-doctoral researcher and physician in the lab of R. G. Kühl and A. G. Siegmund at Charité, Universitätsmedizin Berlin, investigating the mechanisms behind inflammation in Inflammatory Bowel Disease patients.

scholarly journals First person – Srija Bhagavatula

2021 ◽  
Vol 134 (2) ◽  
pp. jcs258327
Keyword(s):  
Cell Biology ◽  
Mrna Localization ◽  
Early Career ◽  
First Person ◽  
Max Planck ◽  
Stem Loop ◽  
Early Career Researchers ◽  
Stem Loop Structure ◽  
Molecular Cell Biology ◽  
Selection Of

ABSTRACTFirst Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Srija Bhagavatula is first author on ‘A putative stem-loop structure in Drosophila crumbs is required for mRNA localisation in epithelia and germline cells’, published in JCS. Srija is a post-doc in the lab of Dr Elisabeth Knust at Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany, investigating the significance of mRNA localization in epithelia.

scholarly journals First person – Anna Dowbaj

2021 ◽  
Vol 134 (13) ◽  
Keyword(s):  
Cell Biology ◽  
Early Career ◽  
First Person ◽  
Max Planck ◽  
Early Career Researchers ◽  
Francis Crick ◽  
Molecular Cell Biology ◽  
Cell Niche ◽  
Selection Of

ABSTRACT First Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Anna Dowbaj is co-first author on ‘ An optogenetic method for interrogating YAP1 and TAZ nuclear–cytoplasmic shuttling’, published in JCS. Anna conducted the work described in this article while a PhD student in the lab of Erik Sahai at the Francis Crick Institute, London, UK. She is now a postdoc in the lab of Meritxell Huch at the Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany, investigating the role of the stem cell niche in liver regeneration.

Application of FRAP and digitized fluorescence microscopy to problems in cell membrane dynamics

1988 ◽  
Vol 46 ◽  
pp. 118-119
Author(s):  
K. Jacobson ◽  
A. Ishihara ◽  
B. Holifield ◽  
F. Zhang
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Biology ◽  
Extended Period ◽  
Dynamic Structure ◽  
Living Cells ◽  
Membrane Dynamics ◽  
Molecular Cell Biology ◽  
Image Averaging ◽  
Single Living Cells ◽  
Single Living

Our laboratory is concerned with understanding the dynamic structure of the plasma membrane with particular reference to the movement of membrane constituents during cell locomotion. In addition to the standard tools of molecular cell biology, we employ both fluorescence recovery after photo- bleaching (FRAP) and digitized fluorescence microscopy (DFM) to investigate individual cells. FRAP allows the measurement of translational mobility of membrane and cytoplasmic molecules in small regions of single, living cells. DFM is really a new form of light microscopy in that the distribution of individual classes of ions, molecules, and macromolecules can be followed in single, living cells. By employing fluorescent antibodies to defined antigens or fluorescent analogs of cellular constituents as well as ultrasensitive, electronic image detectors and video image averaging to improve signal to noise, fluorescent images of living cells can be acquired over an extended period without significant fading and loss of cell viability.

Animal taxa named for Rollo H. Beck

2014 ◽  
Vol 41 (1) ◽  
pp. 113-123 ◽  
Author(s):  
Gary M. Fellers
Keyword(s):  
Natural History ◽  
South America ◽  
Southern California ◽  
American Museum ◽  
Type Specimens ◽  
California Academy ◽  
Vertebrate Zoology ◽  
The Caribbean ◽  
Set Up ◽  
Academy Of Sciences

Rollo Howard Beck (1870–1950) was a professional bird collector who spent most of his career on expeditions to the Channel Islands off southern California, the Galápagos Islands, South America, the South Pacific, and the Caribbean. Some of the expeditions lasted as long as ten years during which time he and his wife, Ida, were often working in primitive conditions on sailing vessels or camps set up on shore. Throughout these expeditions, Beck collected specimens for the California Academy of Sciences, the Museum of Vertebrate Zoology at Berkeley (California), the American Museum of Natural History, and the Walter Rothschild Museum at Tring, England. Beck was one of the premier collectors of his time and his contributions were recognized by having 17 taxa named becki in his honor. Of these taxa, Beck collected 15 of the type specimens.

Breeding study of sea buckthorn (Hippophae rhamnoides L.) in the Institute of Horticulture, NAAS оf Ukraine

2020 ◽  
pp. 37-49
Author(s):  
V.V. Moskalets ◽  
◽  
T.Z. Moskalets ◽  
I.V. Grynyk ◽  
O.A. Shevchuk ◽  
...  
Keyword(s):  
Genetic Markers ◽  
Cell Biology ◽  
Molecular Genetic ◽  
The State ◽  
Sea Buckthorn ◽  
National Academy Of Sciences ◽  
Scientific Cooperation ◽  
State Register ◽  
Academy Of Sciences

The authors present the results of the sea buckthorn breeding at the Institute of Horticulture (NAAS). The stages of the work have been analyzed – from studying and selecting the initial material in the conditions of the Polissya, Polissya-Lisosteppe and Lisosteppe ecotopes (2012-2016) to the successful targeted introduction to the Northern part of the Lisosteppe (2017-2019) and new forms have been characterized according to the traits valuable for economy and molec-ular genetic markers. The new forms of the researched crop taking into consideration the high indices of their productiv-ity,adaptivity to the unfavourable abiotic and biotic environmental factors and consumption quality of fruits for pro-cessing and making functionary products were entered officially into Genetic Fund of the Plants of Ukraine as con-firmed by the certificates of copyright and developed genetic passports. The list of these genotypes includes 1-15-1 (Nos-ivchanka, UA3700073), 1-15-8S (Mitsna, UA3700079), make form 1-15-6Ch (Aboryhen 6/11, UA3700080), 1-15-9 Ka-rotynna, UA3700082), 1-15-3 (Pamiatka, UA3700076), 1-15-8V (Soniachne siayvo, UA3700075), 1-15-11 (Lymonna, UA3700072), 2-15-73 (Morkviana, UA3700077), 1-15-5 (Adaptyvna, UA3700078), 1-15-8B (Osoblyva, UA3700083), 1-15-6 (Apelsynova, UA3700084) and forms 6A/11 (UA3700081), 1-15-5a (Sribnolysta 5a, UA3700074). The possibility of using 5 DNA markers to characterize genotypes of sea buckthorn bymeans of the molecular genetic markers was tested and evaluated in the framework of the scientific cooperation with the Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine. It should be noted that the most polymorphic markers were HrMS025 and HrMS026. However, the marker HrMS014 was monomorphic, but appeared in all the samples, so it can be used as a reference. The best forms of sea buckthorn Adaptyvna (certificate №190899) and Osoblyva (certificate №190900) were included into the State Register of Plant Varieties Suitable for dissemination in Ukraine, and the cultivars of the univer-sal use Nadiina (applications №18299010), Oliana (applications №18299009) and Morkviana (applications № 20299001) and cv pollinator Obrii (applications №18299008) undergo the State strain test. The attention is concentrat-ed on the promising directions of the new sea buckthorn genotypes for the prior breeding and genetic investigations at the Institute of Horticulture (NAAS) and its network.

scholarly journals Versatile CRISPR/Cas9 Systems for Genome Editing in Ustilago maydis

2021 ◽  
Vol 7 (2) ◽  
pp. 149
Author(s):  
Sarah-Maria Wege ◽  
Katharina Gejer ◽  
Fabienne Becker ◽  
Michael Bölker ◽  
Johannes Freitag ◽  
...  
Keyword(s):  
Genome Editing ◽  
Cell Biology ◽  
Gene Deletion ◽  
Fluorescent Protein ◽  
Point Mutations ◽  
Model Organism ◽  
Ustilago Maydis ◽  
Genomic Locus ◽  
Targeted Gene Deletion ◽  
Molecular Cell Biology

The phytopathogenic smut fungus Ustilago maydis is a versatile model organism to study plant pathology, fungal genetics, and molecular cell biology. Here, we report several strategies to manipulate the genome of U. maydis by the CRISPR/Cas9 technology. These include targeted gene deletion via homologous recombination of short double-stranded oligonucleotides, introduction of point mutations, heterologous complementation at the genomic locus, and endogenous N-terminal tagging with the fluorescent protein mCherry. All applications are independent of a permanent selectable marker and only require transient expression of the endonuclease Cas9hf and sgRNA. The techniques presented here are likely to accelerate research in the U. maydis community but can also act as a template for genome editing in other important fungi.

Interleukin-11: Review of Molecular, Cell Biology, and Clinical Use

Blood ◽  
1997 ◽  
Vol 89 (11) ◽  
pp. 3897-3908 ◽  
Author(s):  
Xunxiang Du ◽  
David A. Williams
Keyword(s):  
Cell Biology ◽  
Clinical Use ◽  
Molecular Cell Biology ◽  
Interleukin 11
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