scholarly journals The molecular chaperone Hsp90 regulates heterochromatin assembly through stabilizing multiple complexes in fission yeast

2020 ◽  
Vol 133 (13) ◽  
pp. jcs244863
Author(s):  
Li Sun ◽  
Xiao-Min Liu ◽  
Wen-Zhu Li ◽  
Yuan-Yuan Yi ◽  
Xiangwei He ◽  
...  
Keyword(s):  
Fission Yeast ◽  
Molecular Chaperone ◽  
Heterochromatin Assembly

Two different Argonaute complexes are required for siRNA generation and heterochromatin assembly in fission yeast

2007 ◽  
Vol 14 (3) ◽  
pp. 200-207 ◽  
Author(s):  
Shane M Buker ◽  
Tetsushi Iida ◽  
Marc Bühler ◽  
Judit Villén ◽  
Steven P Gygi ◽  
...  
Keyword(s):  
Fission Yeast ◽  
Heterochromatin Assembly

Swi6/HP1 binding to RNA-DNA hybrids initiates heterochromatin assembly

2020 ◽  
Author(s):  
Jagmohan Singh ◽  
Jyotsna Kumar ◽  
Swati Haldar ◽  
Neelima Gupta ◽  
Viney Kumar ◽  
...  
Keyword(s):  
Fission Yeast ◽  
Rnai Pathway ◽  
Affinity Binding ◽  
Heterochromatin Formation ◽  
Independent Mechanism ◽  
Alternate Model ◽  
Self Association ◽  
Heterochromatin Silencing ◽  
Heterochromatin Structure ◽  
Heterochromatin Assembly

Abstract Heterochromatin formation in fission yeast and metazoans involves di/trimethylation of histone H3 at lysine 9 position (me2/me3-K9-H3) by the histone methyltransferase (HMT) Suv39/Clr4, followed by binding of Swi6/HP1 to me2/me3-K9-H3 via its chromodomain1. Subsequent self-association of Swi6/HP1 on adjacent nucleosomes leads to folded heterochromatin structure1-3. An alternate model suggests a concerted participation of Clr4 and Swi6/HP12,3. HP1 binding to RNA has been invoked for heterochromatin silencing in metazoans4,5. Swi6/HP1 also binds and channels RNA to exosome pathway in fission yeast6. Recruitment of Swi6/HP1 to centromere is also dependent on the RNAi pathway7. Here we show that Swi6/HP1 exhibits binding to RNAs, ranging from promiscuous, low-affinity binding to mRNAs, to moderate-affinity binding to the RNAi-generated siRNAs corresponding to the repeats present in heterochromatin regions7, to high affinity binding to the RNA-DNA hybrids cognate to the repeats. Together with sensitivity of Swi6 localization and silencing to RNaseH, our results suggest a dynamic distribution of Swi6/HP1 among the heterochromatin and euchromatic transcripts and binding to RNA-DNA hybrid as an RNAi-dependent and Me2/me3-K9-H3-independent mechanism of recruitment, leading to heterochromatin formation and silencing.

scholarly journals Interaction of APC/C-E3 Ligase with Swi6/HP1 and Clr4/Suv39 in Heterochromatin Assembly in Fission Yeast

2008 ◽  
Vol 284 (11) ◽  
pp. 7165-7176 ◽  
Author(s):  
Rudra Narayan Dubey ◽  
Nandni Nakwal ◽  
Kamlesh Kumar Bisht ◽  
Ashok Saini ◽  
Swati Haldar ◽  
...  
Keyword(s):  
Fission Yeast ◽  
E3 Ligase ◽  
Heterochromatin Assembly

scholarly journals Nuclear Retention of Fission Yeast Dicer Is a Prerequisite for RNAi-Mediated Heterochromatin Assembly

2010 ◽  
Vol 18 (1) ◽  
pp. 102-113 ◽  
Author(s):  
Stephan Emmerth ◽  
Heiko Schober ◽  
Dimos Gaidatzis ◽  
Tim Roloff ◽  
Kirsten Jacobeit ◽  
...  
Keyword(s):  
Fission Yeast ◽  
Nuclear Retention ◽  
Heterochromatin Assembly

scholarly journals The Proper Splicing of RNAi Factors Is Critical for Pericentric Heterochromatin Assembly in Fission Yeast

PLoS Genetics ◽  
2014 ◽  
Vol 10 (5) ◽  
pp. e1004334 ◽  
Author(s):  
Scott P. Kallgren ◽  
Stuart Andrews ◽  
Xavier Tadeo ◽  
Haitong Hou ◽  
James J. Moresco ◽  
...  
Keyword(s):  
Fission Yeast ◽  
Pericentric Heterochromatin ◽  
Heterochromatin Assembly

scholarly journals Continuous Requirement for the Clr4 Complex But Not RNAi for Centromeric Heterochromatin Assembly in Fission Yeast Harboring a Disrupted RITS Complex

PLoS Genetics ◽  
2010 ◽  
Vol 6 (10) ◽  
pp. e1001174 ◽  
Author(s):  
Sreenath Shanker ◽  
Godwin Job ◽  
Olivia L. George ◽  
Kevin M. Creamer ◽  
Alaa Shaban ◽  
...  
Keyword(s):  
Fission Yeast ◽  
Centromeric Heterochromatin ◽  
Heterochromatin Assembly

scholarly journals Sir2 Regulates Histone H3 Lysine 9 Methylation and Heterochromatin Assembly in Fission Yeast

2003 ◽  
Vol 13 (14) ◽  
pp. 1240-1246 ◽  
Author(s):  
Gurumurthy D. Shankaranarayana ◽  
Mohammad R. Motamedi ◽  
Danesh Moazed ◽  
Shiv I.S. Grewal
Keyword(s):  
Fission Yeast ◽  
Histone H3 ◽  
Heterochromatin Assembly
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