scholarly journals Inhibition of Notch signaling by the p105 and p180 subunits of Drosophila chromatin assembly factor 1 is required for follicle cell proliferation

2019 ◽  
Vol 132 (2) ◽  
pp. jcs224170 ◽  
Author(s):  
Pang-Kuo Lo ◽  
Yi-Chun Huang ◽  
David Corcoran ◽  
Renjie Jiao ◽  
Wu-Min Deng
Keyword(s):  
Cell Proliferation ◽  
Notch Signaling ◽  
Follicle Cell ◽  
Chromatin Assembly ◽  
Chromatin Assembly Factor ◽  
Assembly Factor

scholarly journals A separable domain of the p150 subunit of human chromatin assembly factor-1 promotes protein and chromosome associations with nucleoli

2014 ◽  
Vol 25 (18) ◽  
pp. 2866-2881 ◽  
Author(s):  
Corey L. Smith ◽  
Timothy D. Matheson ◽  
Daniel J. Trombly ◽  
Xiaoming Sun ◽  
Eric Campeau ◽  
...  
Keyword(s):  
Repetitive Element ◽  
Chromatin Assembly ◽  
The Other ◽  
Dna Interactions ◽  
Interaction Sites ◽  
Chromatin Assembly Factor ◽  
Nucleolar Proteins ◽  
Nucleolar Chromosome ◽  
Assembly Factor ◽  
Chromosome Associations

Chromatin assembly factor-1 (CAF-1) is a three-subunit protein complex conserved throughout eukaryotes that deposits histones during DNA synthesis. Here we present a novel role for the human p150 subunit in regulating nucleolar macromolecular interactions. Acute depletion of p150 causes redistribution of multiple nucleolar proteins and reduces nucleolar association with several repetitive element–containing loci. Of note, a point mutation in a SUMO-interacting motif (SIM) within p150 abolishes nucleolar associations, whereas PCNA or HP1 interaction sites within p150 are not required for these interactions. In addition, acute depletion of SUMO-2 or the SUMO E2 ligase Ubc9 reduces α-satellite DNA association with nucleoli. The nucleolar functions of p150 are separable from its interactions with the other subunits of the CAF-1 complex because an N-terminal fragment of p150 (p150N) that cannot interact with other CAF-1 subunits is sufficient for maintaining nucleolar chromosome and protein associations. Therefore these data define novel functions for a separable domain of the p150 protein, regulating protein and DNA interactions at the nucleolus.

scholarly journals Essential Role of Chromatin Assembly Factor-1–mediated Rapid Nucleosome Assembly for DNA Replication and Cell Division in Vertebrate Cells

2007 ◽  
Vol 18 (1) ◽  
pp. 129-141 ◽  
Author(s):  
Yasunari Takami ◽  
Tatsuya Ono ◽  
Tatsuo Fukagawa ◽  
Kei-ichi Shibahara ◽  
Tatsuo Nakayama
Keyword(s):  
Dna Replication ◽  
Essential Role ◽  
Chromatin Assembly ◽  
Nuclear Antigen ◽  
Nucleosome Assembly ◽  
Chromatin Assembly Factor ◽  
Assembly Factor

Chromatin assembly factor-1 (CAF-1), a complex consisting of p150, p60, and p48 subunits, is highly conserved from yeast to humans and facilitates nucleosome assembly of newly replicated DNA in vitro. To investigate roles of CAF-1 in vertebrates, we generated two conditional DT40 mutants, respectively, devoid of CAF-1p150 and p60. Depletion of each of these CAF-1 subunits led to delayed S-phase progression concomitant with slow DNA synthesis, followed by accumulation in late S/G2 phase and aberrant mitosis associated with extra centrosomes, and then the final consequence was cell death. We demonstrated that CAF-1 is necessary for rapid nucleosome formation during DNA replication in vivo as well as in vitro. Loss of CAF-1 was not associated with the apparent induction of phosphorylations of S-checkpoint kinases Chk1 and Chk2. To elucidate the precise role of domain(s) in CAF-1p150, functional dissection analyses including rescue assays were preformed. Results showed that the binding abilities of CAF-1p150 with CAF-1p60 and DNA polymerase sliding clamp proliferating cell nuclear antigen (PCNA) but not with heterochromatin protein HP1-γ are required for cell viability. These observations highlighted the essential role of CAF-1–dependent nucleosome assembly in DNA replication and cell proliferation through its interaction with PCNA.

Chromatin Assembly Factor-1/p60 overexpression: a potential index of psoriasis severity

2014 ◽  
Vol 24 (4) ◽  
pp. 509-511 ◽  
Author(s):  
Massimo Mascolo ◽  
Fabio Ayala ◽  
Gennaro Ilardi ◽  
Anna Balato ◽  
Serena Lembo
Keyword(s):  
Chromatin Assembly ◽  
Chromatin Assembly Factor ◽  
Potential Index ◽  
Assembly Factor ◽  
Psoriasis Severity

scholarly journals Chromatin Assembly Factor 1 (CAF-1) facilitates the establishment of facultative heterochromatin during pluripotency exit

2019 ◽  
Vol 47 (21) ◽  
pp. 11114-11131 ◽  
Author(s):  
Liang Cheng ◽  
Xu Zhang ◽  
Yan Wang ◽  
Haiyun Gan ◽  
Xiaowei Xu ◽  
...  
Keyword(s):  
Cell Fate ◽  
Embryonic Stem ◽  
Chromatin Assembly ◽  
Nuclear Antigen ◽  
Gene Promoters ◽  
Cell Fate Determination ◽  
Nucleosome Assembly ◽  
Fate Determination ◽  
Chromatin Assembly Factor ◽  
Assembly Factor

Abstract Establishment and subsequent maintenance of distinct chromatin domains during embryonic stem cell (ESC) differentiation are crucial for lineage specification and cell fate determination. Here we show that the histone chaperone Chromatin Assembly Factor 1 (CAF-1), which is recruited to DNA replication forks through its interaction with proliferating cell nuclear antigen (PCNA) for nucleosome assembly, participates in the establishment of H3K27me3-mediated silencing during differentiation. Deletion of CAF-1 p150 subunit impairs the silencing of many genes including Oct4, Sox2 and Nanog as well as the establishment of H3K27me3 at these gene promoters during ESC differentiation. Mutations of PCNA residues involved in recruiting CAF-1 to the chromatin also result in defects in differentiation in vitro and impair early embryonic development as p150 deletion. Together, these results reveal that the CAF-1-PCNA nucleosome assembly pathway plays an important role in the establishment of H3K27me3-mediated silencing during cell fate determination.

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