scholarly journals Greatwall kinase at a glance

2018 ◽  
Vol 131 (20) ◽  
pp. jcs222364 ◽  
Author(s):  
Anna Castro ◽  
Thierry Lorca
Keyword(s):  
Greatwall Kinase

scholarly journals Greatwall Kinase Is Required for Meiotic Maturation in Porcine Oocytes1

2013 ◽  
Vol 89 (3) ◽  
Author(s):  
Ying-Hua Li ◽  
Hyoeun Kang ◽  
Yong-Nan Xu ◽  
Young-Tae Heo ◽  
Xiang-Shun Cui ◽  
...  
Keyword(s):  
Meiotic Maturation ◽  
Greatwall Kinase

scholarly journals Loss of the Greatwall Kinase Weakens the Spindle Assembly Checkpoint

PLoS Genetics ◽  
2016 ◽  
Vol 12 (9) ◽  
pp. e1006310 ◽  
Author(s):  
M. Kasim Diril ◽  
Xavier Bisteau ◽  
Mayumi Kitagawa ◽  
Matias J. Caldez ◽  
Sheena Wee ◽  
...  
Keyword(s):  
Spindle Assembly Checkpoint ◽  
Spindle Assembly ◽  
Greatwall Kinase

scholarly journals The Greatwall kinase: a new pathway in the control of the cell cycle

Oncogene ◽  
2012 ◽  
Vol 32 (5) ◽  
pp. 537-543 ◽  
Author(s):  
T Lorca ◽  
A Castro
Keyword(s):  
Cell Cycle ◽  
Greatwall Kinase ◽  
Kinase A

scholarly journals Cyclin A triggers Mitosis either via the Greatwall kinase pathway or Cyclin B

2020 ◽  
Vol 39 (11) ◽  
Author(s):  
Nadia Hégarat ◽  
Adrijana Crncec ◽  
Maria F Suarez Peredo Rodriguez ◽  
Fabio Echegaray Iturra ◽  
Yan Gu ◽  
...  
Keyword(s):  
Cyclin A ◽  
Cyclin B ◽  
Greatwall Kinase ◽  
Kinase Pathway

scholarly journals Investigating the determinants of mammalian Mastl kinase activation

2020 ◽  
Author(s):  
Mehmet Erguven ◽  
M. Kasim Diril
Keyword(s):  
Expression System ◽  
Activation Loop ◽  
Middle Region ◽  
Kinase Activation ◽  
Viral Expression ◽  
Egg Extracts ◽  
Xenopus Egg ◽  
Greatwall Kinase ◽  
Xenopus Egg Extracts ◽  
Atypical Member

ABSTRACTMastl (Greatwall) kinase is an essential mitotic protein kinase. Mastl is an atypical member of AGC family with a unique long stretch of non-conserved middle region. The mechanism of its phosphorylation dependent activation has been studied in Xenopus egg extracts, revealing several phosphosites that were suggested to be crucial for kinase activation. These residues correspond to T193 and T206 in the activation loop, and S861 in the C-tail of mouse Mastl. By combining a chemically inducible knockout system to deplete the endogenous Mastl and a viral expression system to ectopically express the mutant variants, we obtained a viable knockout clone that expresses the S861A and S861D mutants. We observed that proliferation rates of the MastlS861A and MastlS861D clones were comparable. Our results have revealed that phosphorylation of the turn motif phosphosite (S861) is auxiliary and it is not indispensable for Mastl function.

scholarly journals Cyclin A triggers Mitosis either via Greatwall or Cyclin B

2018 ◽  
Author(s):  
Nadia Hégarat ◽  
Adrijana Crncec ◽  
Maria F. Suarez Peredoa Rodri-guez ◽  
Fabio Echegaray Iturra ◽  
Yan Gu ◽  
...  
Keyword(s):  
Cyclin A ◽  
Cyclin B ◽  
Mitotic Progression ◽  
Nuclear Envelope Breakdown ◽  
Distinct Subset ◽  
Initial Activation ◽  
Greatwall Kinase ◽  
G2 Phase ◽  
Higher Eukaryotes ◽  
Complete Cell

AbstractTwo mitotic Cyclins, A and B, exist in higher eukaryotes, but their specialised functions in mitosis are poorly understood. Using degron tags we analyse how acute depletion of these proteins affects mitosis. Loss of Cyclin A in G2-phase prevents the initial activation of Cdk1. Cells lacking Cyclin B can enter mitosis and phosphorylate most mitotic proteins, because of parallel PP2A:B55 phos-phatase inactivation by Greatwall kinase. The final barrier to mitotic establishment corresponds to nuclear envelope breakdown that requires a decisive shift in the balance of Cdk1 and PP2A:B55 activity. Beyond this point Cyclin B/Cdk1 is essential to phosphorylate a distinct subset mitotic Cdk1 substrates that are essential to complete cell division. Our results identify how Cyclin A, B and Greatwall coordinate mitotic progression by increasing levels of Cdk1-dependent substrate phos-phorylation.

scholarly journals Author response: Fcp1 phosphatase controls Greatwall kinase to promote PP2A-B55 activation and mitotic progression

2015 ◽  
Author(s):  
Rosa Della Monica ◽  
Roberta Visconti ◽  
Nando Cervone ◽  
Angela Flavia Serpico ◽  
Domenico Grieco
Keyword(s):  
Author Response ◽  
Mitotic Progression ◽  
Greatwall Kinase
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