Cytoplasmic streaming in Chara corallina studied by laser light scattering

1976 ◽  
Vol 22 (3) ◽  
pp. 633-643
Author(s):  
D.B. Sattelle ◽  
P.B. Buchan
Keyword(s):  
Electrical Stimulation ◽  
Light Scattering ◽  
Light Microscope ◽  
Cytoplasmic Streaming ◽  
Laser Light ◽  
Algal Cell ◽  
Chara Corallina ◽  
Laser Light Scattering ◽  
Laser Doppler Velocimeter

An apparatus is described by means of which the power versus frequency spectrum of the photomultiplier current can be obtained for laser light scattered by streaming cytoplasm in the algal cell Chara corallina. A Doppler peak is noted in the spectrum which is abolished when cytoplasmic streaming is arrested by electrical stimulation. For 5 cells of Chara, this simple laser-Doppler velocimeter gave streaming velocities (46-7 mum s-1, S.D. +/− 4–8 at 20 degrees C) similar to those obtained for the same cells using the light microscope (44-3 mum s-1, S.D. +/− 5-3 at 20 degrees C). A narrow distribution of streaming velocities is indicated. The technique described provides a rapid, quantitative assay of the in vivo rheological properties of cytoplasm.

In Vivo Measurement of Human Nasal Mucociliary Motility Using a Laser Light Scattering Instrument

1997 ◽  
Vol 106 (10) ◽  
pp. 859-862 ◽  
Author(s):  
Yoav Paltieli ◽  
Ludwig Podoshin ◽  
Milo Fradis ◽  
Hala Shiti ◽  
Jacob Ben-David ◽  
...  
Keyword(s):  
Light Scattering ◽  
Defense Mechanisms ◽  
Laser Light ◽  
Normal Subjects ◽  
Laser Light Scattering ◽  
In Vivo Measurement ◽  
Fiberoptic Probe ◽  
The Mean ◽  
User Friendly

The mucociliary system is one of the most important airway defense mechanisms, and knowledge of the mucociliary wave frequency (MWF) is important in the understanding of this system. Employing a laser light scattering technique and a thin, flexible fiberoptic probe, we developed and tested a simple and practical device for real-time in vivo measurements of mucociliary activity in the human nose. The laser instrument is user-friendly and does not produce any discomfort to the patient. The mean ± SE of MWF of 36 measurements in 16 normal subjects was 7.7 ± 0.5 Hz. The mean MWF of 17 measurements in 7 patients with allergic rhinitis was 5.5 ± 0.2 Hz (p < .005), and the mean MWF of 56 measurements in 17 patients with septum deviation was 5.8 ± 0.2 Hz (p < .001). The instrument presented in this study might provide a new and convenient method of studying the mucociliary activity in the respiratory tract.

A New System to Detect Native Microaggregates of Platelets In Vivo, with a Novel Platelet Aggregometer Employing Laser Light Scattering

1998 ◽  
Vol 92 (5) ◽  
pp. 221-228 ◽  
Author(s):  
Yasuhiro Tomida ◽  
Satoshi Iino ◽  
Masakatsu Nishikawa ◽  
Hiroyoshi Hidaka
Keyword(s):  
Light Scattering ◽  
Laser Light ◽  
Laser Light Scattering ◽  
New System

Assessment of whole blood structure and dynamics in vitro and in vivo by laser light scattering

1996 ◽  
Author(s):  
Alexander V. Priezzhev ◽  
Olga M. Ryaboshapka ◽  
Natalia B. Savchenko ◽  
Nikolai N. Firsov ◽  
Vladimir V. Kolinko
Keyword(s):  
Light Scattering ◽  
Whole Blood ◽  
Laser Light ◽  
Laser Light Scattering ◽  
Structure And Dynamics

In-Situ Dual-Wavelength Ellipsometry and Light Scattering Monitoring of Si/Si1−xGex Heterostructures and Multiuantum Wells

1993 ◽  
Vol 324 ◽  
Author(s):  
C. Pickering ◽  
D.A.O. Hope ◽  
W.Y. Leong ◽  
D.J. Robbins ◽  
R. Greef
Keyword(s):  
Growth Rate ◽  
Light Scattering ◽  
Laser Light ◽  
Growth Period ◽  
Laser Light Scattering ◽  
Heterojunction Bipolar Transistor ◽  
Dual Wavelength ◽  
Multi Quantum Well ◽  
Refractive Index Data

AbstractIn-situ dual-wavelength ellipsometry and laser light scattering have been used to monitor growth of Si/Si1−x,Gex heterojunction bipolar transistor and multi-quantum well (MQW) structures. The growth rate of B-doped Si0 8Ge0.2 has been shown to be linear, but that of As-doped Si is non-linear, decreasing with time. Refractive index data have been obtained at the growth temperature for x = 0.15, 0.20, 0.25. Interface regions ∼ 6-20Å thickness have been detected at hetero-interfaces and during interrupted alloy growth. Period-to-period repeatability of MQW structures has been shown to be ±lML.

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