scholarly journals Haspin kinase regulates microtubule-organizing center clustering and stability through Aurora kinase C in mouse oocytes

2016 ◽  
Vol 129 (19) ◽  
pp. 3648-3660 ◽  
Author(s):  
Ahmed Z. Balboula ◽  
Alexandra L. Nguyen ◽  
Amanda S. Gentilello ◽  
Suzanne M. Quartuccio ◽  
David Drutovic ◽  
...  
Keyword(s):  
Aurora Kinase ◽  
Microtubule Organizing Center ◽  
Mouse Oocytes ◽  
Kinase C ◽  
Organizing Center

scholarly journals PLK1 is required for chromosome compaction and microtubule organization in mouse oocytes

2020 ◽  
Vol 31 (12) ◽  
pp. 1206-1217
Author(s):  
Tara M. Little ◽  
Philip W. Jordan
Keyword(s):  
Chromosome Segregation ◽  
Microtubule Organizing Center ◽  
Microtubule Organization ◽  
Spindle Formation ◽  
Bipolar Spindle ◽  
Mouse Oocytes ◽  
Organizing Center ◽  
Meiosis I

By deleting Plk1 in mouse oocytes before meiotic resumption, we show that PLK1 is essential for the formation of condensed bivalent chromosomes, microtubule organizing center fragmentation, liquid-like spindle domain localization, and bipolar spindle formation. Thus, PLK1 coordinates processes that ensure chromosome segregation during meiosis I.

scholarly journals The Transcription Factor Egr3 Is a Putative Component of the Microtubule Organizing Center in Mouse Oocytes

PLoS ONE ◽  
2014 ◽  
Vol 9 (4) ◽  
pp. e94708 ◽  
Author(s):  
Hyejin Shin ◽  
Sojung Kwon ◽  
Haengseok Song ◽  
Hyunjung Jade Lim
Keyword(s):  
Transcription Factor ◽  
Microtubule Organizing Center ◽  
Mouse Oocytes ◽  
Organizing Center

Three-Dimensional reconstruction of isolated centrosomes by automated electron tomography

1994 ◽  
Vol 52 ◽  
pp. 310-311
Author(s):  
M.B. Braunfeld ◽  
M. Moritz ◽  
B.M. Alberts ◽  
J.W. Sedat ◽  
D.A. Agard
Keyword(s):  
Electron Tomography ◽  
Three Dimensional ◽  
Vital Role ◽  
Complex Nature ◽  
Animal Cells ◽  
Microtubule Organizing Center ◽  
Nucleation Sites ◽  
Dimensional Reconstruction ◽  
Organizing Center ◽  
Resolution Model

In animal cells, the centrosome functions as the primary microtubule organizing center (MTOC). As such the centrosome plays a vital role in determining a cell's shape, migration, and perhaps most importantly, its division. Despite the obvious importance of this organelle little is known about centrosomal regulation, duplication, or how it nucleates microtubules. Furthermore, no high resolution model for centrosomal structure exists.We have used automated electron tomography, and reconstruction techniques in an attempt to better understand the complex nature of the centrosome. Additionally we hope to identify nucleation sites for microtubule growth.Centrosomes were isolated from early Drosophila embryos. Briefly, after large organelles and debris from homogenized embryos were pelleted, the resulting supernatant was separated on a sucrose velocity gradient. Fractions were collected and assayed for centrosome-mediated microtubule -nucleating activity by incubating with fluorescently-labeled tubulin subunits. The resulting microtubule asters were then spun onto coverslips and viewed by fluorescence microscopy.

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