scholarly journals Traction forces mediated by integrin signaling are necessary for definitive endoderm specification

2015 ◽  
Vol 128 (10) ◽  
pp. 1961-1968 ◽  
Author(s):  
H. Taylor-Weiner ◽  
N. Ravi ◽  
A. J. Engler
Keyword(s):  
Definitive Endoderm ◽  
Integrin Signaling ◽  
Traction Forces

scholarly journals Traction forces mediated by integrin signaling are necessary for definitive endoderm specification

Development ◽  
2015 ◽  
Vol 142 (11) ◽  
pp. e1106-e1106
Author(s):  
H. Taylor-Weiner ◽  
N. Ravi ◽  
A. J. Engler
Keyword(s):  
Definitive Endoderm ◽  
Integrin Signaling ◽  
Traction Forces

Video-microscopic measurement of cell-substratum traction forces generated by locomoting keratocytes

1993 ◽  
Vol 51 ◽  
pp. 188-189
Author(s):  
Tim Oliver ◽  
Michelle Leonard ◽  
Juliet Lee ◽  
Akira Ishihara ◽  
Ken Jacobson
Keyword(s):  
Silicone Rubber ◽  
Molecular Motors ◽  
Video Frame ◽  
Traction Forces ◽  
Cell Traction Forces ◽  
Latex Beads ◽  
Cell Traction ◽  
Local Cell ◽  
Microscopic Measurement ◽  
Kinetics Of

We are using video-enhanced light microscopy to investigate the pattern and magnitude of forces that fish keratocytes exert on flexible silicone rubber substrata. Our goal is a clearer understanding of the way molecular motors acting through the cytoskeleton co-ordinate their efforts into locomotion at cell velocities up to 1 μm/sec. Cell traction forces were previously observed as wrinkles(Fig.l) in strong silicone rubber films by Harris.(l) These forces are now measureable by two independant means.In the first of these assays, weakly crosslinked films are made, into which latex beads have been embedded.(Fig.2) These films report local cell-mediated traction forces as bead displacements in the plane of the film(Fig.3), which recover when the applied force is released. Calibrated flexible glass microneedles are then used to reproduce the translation of individual beads. We estimate the force required to distort these films to be 0.5 mdyne/μm of bead movement. Video-frame analysis of bead trajectories is providing data on the relative localisation, dissipation and kinetics of traction forces.

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