scholarly journals The meiosis-specific nuclear passenger protein is required for proper assembly of forespore membrane in fission yeast

2014 ◽  
Vol 127 (20) ◽  
pp. 4429-4442 ◽  
Author(s):  
Masak Takaine ◽  
Kazuki Imada ◽  
Osamu Numata ◽  
Taro Nakamura ◽  
Kentaro Nakano
Keyword(s):  
Fission Yeast ◽  
Passenger Protein

scholarly journals Suppressors of Bir1p (Survivin) Identify Roles for the Chromosomal Passenger Protein Pic1p (INCENP) and the Replication Initiation Factor Psf2p in Chromosome Segregation

2005 ◽  
Vol 25 (20) ◽  
pp. 9000-9015 ◽  
Author(s):  
Han-Kuei Huang ◽  
Julie M. Bailis ◽  
Joel D. Leverson ◽  
Eliana B. Gómez ◽  
Susan L. Forsburg ◽  
...  
Keyword(s):  
Fission Yeast ◽  
Chromosome Segregation ◽  
Copy Number ◽  
Replication Initiation ◽  
High Copy Number ◽  
Temperature Sensitive ◽  
Chromosome Missegregation ◽  
Chromosomal Passenger ◽  
Sensitive Allele ◽  
Passenger Protein

ABSTRACT Fission yeast Bir1p/Cut17p/Pbh1p, the homolog of human Survivin, is a conserved chromosomal passenger protein that is required for cell division and cytokinesis. To study how Bir1p promotes accurate segregation of chromosomes, we generated and analyzed a temperature-sensitive allele, bir1-46, and carried out genetic screens to find genes that interact with bir1 + . We identified Psf2p, a component of the GINS complex required for DNA replication initiation, as a high-copy-number suppressor of the bir1-46 growth defect. Loss of Psf2p function by depletion or deletion or by use of a temperature-sensitive allele, psf2-209, resulted in chromosome missegregation that was associated with mislocalization of Bir1p. We also found that the human homolog of Psf2p, PSF2, was required for proper chromosome segregation. In addition, we observed that high-copy-number expression of Pic1p, the fission yeast homolog of INCENP (inner centromere protein), suppressed bir1-46. Pic1p exhibited a localization pattern typical of chromosomal passenger proteins. Deletion of pic1 + caused chromosome missegregation phenotypes similar to those of bir1-46. Our data suggest that Bir1p and Pic1p act as part of a conserved chromosomal passenger complex and that Psf2p/GINS indirectly affects the localization and function of this complex in chromosome segregation, perhaps through an S-phase role in centromere replication.

Rapid and reliable preparation of total cell lysates from fission yeast

2006 ◽  
Author(s):  
Atsuko Shirai ◽  
Akihisa Matsuyama ◽  
Yoko Yashiroda ◽  
Ritsuko Arai ◽  
Minoru Yoshida
Keyword(s):  
Fission Yeast ◽  
Total Cell ◽  
Cell Lysates
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