The Location of Blood Group Antigen a on Cultured Rabbit Kidney Cells as Revealed by Ferritin-Labelled Antibody

1972 ◽  
Vol 10 (2) ◽  
pp. 525-533
Author(s):  
ELIZABETH DIMMOCK ◽  
D. FRANKS ◽  
AUDREY M. GLAUERT
Keyword(s):  
High Resolution ◽  
Cell Surface ◽  
Blood Group ◽  
Blood Group Antigen ◽  
Rabbit Kidney ◽  
Kidney Cells ◽  
Specific Staining ◽  
Antigen A

Fluorescein- and ferritin-labelled antibodies were used to locate blood group antigen A on the surfaces of cultured rabbit kidney cells (RK 13). Fluorescent anti-A revealed the antigen on some cells, but not on others. Controls indicated that specific staining could be obtained. Comparable observations were made with ferritin-labelled antibody, and the comparatively high resolution of this method allowed individual antigen molecules to be located. Measurements were made of the distances between the centres of the ferritin molecules and the cell surface, and the position of the blood group antigen relative to the visible components of the membrane is discussed in relation to this.

The Surface Structure of Cultured Rabbit Kidney Cells as Revealed by Electron Microscopy

1970 ◽  
Vol 7 (3) ◽  
pp. 719-737
Author(s):  
ELIZABETH DIMMOCK
Keyword(s):  
Electron Microscopy ◽  
Surface Layer ◽  
Cell Surface ◽  
Blood Group ◽  
Potassium Permanganate ◽  
Ruthenium Red ◽  
Rabbit Kidney ◽  
Dense Layer ◽  
Kidney Cells ◽  
Thin Sections

An epithelioid line of rabbit kidney cells (RK 13), in which the distribution of blood group antigen A had previously been investigated by mixed agglutination, was chosen for studies of the structure of the cell surface. Cells were grown in monolayer culture, and thin sections were examined by electron microscopy after fixation in glutaraldehyde and osmium tetroxide and staining of the sections with lead, or uranyl and lead. Cells were also treated with ruthenium red incorporated in the osmium fixative. Other cells were fixed in lanthanum or potassium permanganate, and both stained and unstained sections were examined. The morphology of RK 13 cell surfaces is described. There is apparently no great degree of cellular specialization. The treatment with ruthenium red resulted in dense staining of a layer of the cell surface that is not visible in conventional preparations, and sometimes in staining of the surfaces of intracellular organelles. Lanthanum permanganate fixation also revealed a dense layer on the surface of the plasma membrane; a less dense surface layer was distinguished in many cells fixed in potassium permanganate. The reaction of ruthenium red with the cell surface is probably due to the presence of acidic glycoproteins, but the chemical specificity of the staining method is not yet clear. The nature of the material revealed by lanthanum and potassium permanaganates is also undefined. However, these staining methods reveal that the cell surface is more complex than is apparent in cells prepared by conventional techniques. The additional surface layer is probably the site of many blood group substances and other compounds involved in the physiological reactions of the cell surface.

A Weak Example of the Blood Group Antigen A

Vox Sanguinis ◽  
1961 ◽  
Vol 6 (2) ◽  
pp. 151-156 ◽  
Author(s):  
B. P. L. Moore ◽  
P. H. Newstead ◽  
Joanne Johnson
Keyword(s):  
Blood Group ◽  
Blood Group Antigen ◽  
Antigen A

A Gene, y, Modifying the Blood Group Antigen A

Vox Sanguinis ◽  
1957 ◽  
Vol 2 (1) ◽  
pp. 25-37
Author(s):  
W. Weiner ◽  
H.B.M. Lewis ◽  
Phyllis Moores ◽  
Ruth Sanger ◽  
R.R. Race
Keyword(s):  
Blood Group ◽  
Blood Group Antigen ◽  
Antigen A

scholarly journals Methodologic and genetic influence on immunohistochemical demonstration and semiquantitation of blood group antigen A in human ureter urothelium.

1985 ◽  
Vol 33 (1) ◽  
pp. 21-26 ◽  
Author(s):  
B R Juhl
Keyword(s):  
Blood Group ◽  
Staining Technique ◽  
Blood Group Antigen ◽  
Blood Type ◽  
Tween 20 ◽  
Immunoperoxidase Staining ◽  
Urothelial Tumors ◽  
Antigen A ◽  
Indirect Immunoperoxidase ◽  
Antigen Loss

In order to improve the accuracy and prognostic value of ABH blood group antigen loss in urothelial tumors, the effect of Lewis blood type and methodologic factors on detectability and distribution of blood group antigen A in human formalin-fixed, paraplast-embedded urothelium and endothelium was investigated by means of the Tween 20-modified indirect immunoperoxidase staining technique. Urothelium of Lea-b+ and Lea-b- individuals expressed significant higher amounts of blood group antigen A compared to urothelium of Lea+b- individuals. The expression on endothelial cells was related to vessel type and size, but not related to Lewis types. Compared to human anti-A, monoclonal anti-A demonstrated blood group antigen A with higher sensitivity and, due to reduced background staining, higher specificity. Consequently monoclonal anti-A detected blood group antigen A in the urothelium of Lea+b- individuals where human anti-A failed to stain, and different staining patterns became apparent. Both a two- to fourfold variation in the proportion between tissue section area and volume, and the volume of anti-A applied induced minor changes in sensitivity and specificity. The monoclonal anti-A method and knowledge about erythrocyte Lewis types might prove valuable in evaluating changes in blood group antigen-A expression in urothelial tumors.

Sign in / Sign up

Export Citation Format

Share Document