scholarly journals S-RNase disrupts tip-localized reactive oxygen species and induces nuclear DNA degradation in incompatible pollen tubes of Pyrus pyrifolia

2010 ◽  
Vol 123 (24) ◽  
pp. 4301-4309 ◽  
Author(s):  
C.-L. Wang ◽  
J. Wu ◽  
G.-H. Xu ◽  
Y.-b. Gao ◽  
G. Chen ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Nuclear Dna ◽  
Reactive Oxygen ◽  
Dna Degradation ◽  
Pollen Tubes ◽  
Pyrus Pyrifolia ◽  
Oxygen Species ◽  
Incompatible Pollen

scholarly journals Acute apoptosis by cisplatin requires induction of reactive oxygen species but is not associated with damage to nuclear DNA

2006 ◽  
Vol 120 (1) ◽  
pp. 175-180 ◽  
Author(s):  
Maria Berndtsson ◽  
Maria Hägg ◽  
Theocharis Panaretakis ◽  
Aleksandra Mandic Havelka ◽  
Maria C. Shoshan ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Nuclear Dna ◽  
Reactive Oxygen ◽  
Oxygen Species

Activation of NF-κB induced by H2O2 and TNF-α and its effects on ICAM-1 expression in endothelial cells

2000 ◽  
Vol 279 (2) ◽  
pp. L302-L311 ◽  
Author(s):  
Andrea L. True ◽  
Arshad Rahman ◽  
Asrar B. Malik
Keyword(s):  
Gene Expression ◽  
Reactive Oxygen Species ◽  
Endothelial Cells ◽  
Nuclear Dna ◽  
Reactive Oxygen Species Generation ◽  
Reactive Oxygen ◽  
Intercellular Adhesion Molecule ◽  
Oxygen Species ◽  
Tnf Α

Reactive oxygen species have been proposed to signal the activation of the transcription factor nuclear factor (NF)-κB in response to tumor necrosis factor (TNF)-α challenge. In the present study, we investigated the effects of H2O2 and TNF-α in mediating activation of NF-κB and transcription of the intercellular adhesion molecule (ICAM)-1 gene. Northern blot analysis showed that TNF-α exposure of human dermal microvascular endothelial cells (HMEC-1) induced marked increases in ICAM-1 mRNA and cell surface protein expression. In contrast, H2O2 added at subcytolytic concentrations failed to activate ICAM-1 expression. Challenge with H2O2 also failed to induce NF-κB-driven reporter gene expression in the transduced HMEC-1 cells, whereas TNF-α increased the NF-κB-driven gene expression ∼10-fold. Gel supershift assay revealed the presence of p65 (Rel A), p50, and c-Rel in both H2O2- and TNF-α-induced NF-κB complexes bound to the ICAM-1 promoter, with the binding of the p65 subunit being the most prominent. In vivo phosphorylation studies, however, showed that TNF-α exposure induced marked phosphorylation of NF-κB p65 in HMEC-1 cells, whereas H2O2 had no effect. These results suggest that reactive oxygen species generation in endothelial cells mediates the binding of NF-κB to nuclear DNA, whereas TNF-α generates additional signals that induce phosphorylation of the bound NF-κB p65 and confer transcriptional competency to NF-κB.

Reactive oxygen species induce signals that lead to apoptotic DNA degradation in primary CD4+ T cells

APOPTOSIS ◽  
2005 ◽  
Vol 10 (6) ◽  
pp. 1433-1443 ◽  
Author(s):  
M. Pajusto ◽  
T. H. Toivonen ◽  
J. Tarkkanen ◽  
E. Jokitalo ◽  
P. S. Mattila
Keyword(s):  
Reactive Oxygen Species ◽  
T Cells ◽  
Cd4 T Cells ◽  
Reactive Oxygen ◽  
Dna Degradation ◽  
Oxygen Species

The apple MdPTI1L kinase is phosphorylated by MdOXI1 during S-RNase-induced reactive oxygen species signaling in pollen tubes

Plant Science ◽  
2021 ◽  
Vol 305 ◽  
pp. 110824
Author(s):  
Chuanbao Wu ◽  
Zhaoyu Gu ◽  
Tianzhong Li ◽  
Jie Yu ◽  
Chunsheng Liu ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Pollen Tubes ◽  
Oxygen Species ◽  
Reactive Oxygen Species Signaling

scholarly journals Arabidopsis ABCG28 is required for the apical accumulation of reactive oxygen species in growing pollen tubes

2019 ◽  
Vol 116 (25) ◽  
pp. 12540-12549 ◽  
Author(s):  
Thanh Ha Thi Do ◽  
Hyunju Choi ◽  
Michael Palmgren ◽  
Enrico Martinoia ◽  
Jae-Ung Hwang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Tip Growth ◽  
Ectopic Expression ◽  
Reactive Oxygen ◽  
Pollen Tubes ◽  
Tube Growth ◽  
Oxygen Species ◽  
Ros Accumulation

Tip-focused accumulation of reactive oxygen species (ROS) is tightly associated with pollen tube growth and is thus critical for fertilization. However, it is unclear how tip-growing cells establish such specific ROS localization. Polyamines have been proposed to function in tip growth as precursors of the ROS, hydrogen peroxide. The ABC transporter AtABCG28 may regulate ROS status, as it contains multiple cysteine residues, a characteristic of proteins involved in ROS homeostasis. In this study, we found that AtABCG28 was specifically expressed in the mature pollen grains and pollen tubes. AtABCG28 was localized to secretory vesicles inside the pollen tube that moved toward and fused with the plasma membrane of the pollen tube tip. Knocking out AtABCG28 resulted in defective pollen tube growth, failure to localize polyamine and ROS to the growing pollen tube tip, and complete male sterility, whereas ectopic expression of this gene in root hair could recover ROS accumulation at the tip and improved the growth under high-pH conditions, which normally prevent ROS accumulation and tip growth. Together, these data suggest that AtABCG28 is critical for localizing polyamine and ROS at the growing tip. In addition, this function of AtABCG28 is likely to protect the pollen tube from the cytotoxicity of polyamine and contribute to the delivery of polyamine to the growing tip for incorporation into the expanding cell wall.

scholarly journals PbrRALF2-elicited reactive oxygen species signaling is mediated by the PbrCrRLK1L13-PbrMPK18 module in pear pollen tubes

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Xiaobing Kou ◽  
Jiangmei Sun ◽  
Peng Wang ◽  
Danqi Wang ◽  
Peng Cao ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Reactive Oxygen ◽  
Pollen Tubes ◽  
Tube Growth ◽  
General Mechanism ◽  
Dependent Manner ◽  
Ros Production ◽  
Oxygen Species

AbstractRapid alkalinization factors (RALFs) are cysteine-rich peptides that play important roles in a variety of biological processes, such as cell elongation and immune signaling. Recent studies in Arabidopsis have shown that RALFs regulate pollen tube growth via plasma membrane receptor-like kinases (RLKs). However, the downstream signal transduction mechanisms of RLKs in pollen tubes are unknown. Here, we identified PbrRALF2, a pear (Pyrus bretschneideri) pollen RALF peptide that inhibits pollen tube growth. We found that PbrRALF2 interacts with a malectin-like domain-containing RLK, PbrCrRLK1L13. The relative affinity between PbrRALF2 and PbrCrRLK1L13 was at the submicromolar level, which is consistent with the values of ligand–receptor kinase pairs and the physiological concentration for PbrRALF2-mediated inhibition of pollen tube growth. After binding to its extracellular domain, PbrRALF2 activated the phosphorylation of PbrCrRLK1L13 in a dose-dependent manner. We further showed that the MAP kinase PbrMPK18 is a downstream target of PbrCrRLK1L13 that mediates PbrRALF2-elicited reactive oxygen species (ROS) production. The excessive accumulation of ROS inhibits pollen tube growth. We show that MPK acts as a mediator for CrRLK1L to stimulate ROS production, which might represent a general mechanism by which RALF and CrRLK1L function in signaling pathways.

scholarly journals Cytotoxic Effect of Curcumin on Malaria Parasite Plasmodium falciparum: Inhibition of Histone Acetylation and Generation of Reactive Oxygen Species

2006 ◽  
Vol 51 (2) ◽  
pp. 488-494 ◽  
Author(s):  
Long Cui ◽  
Jun Miao ◽  
Liwang Cui
Keyword(s):  
Reactive Oxygen Species ◽  
Plasmodium Falciparum ◽  
Cytotoxic Effect ◽  
Nuclear Dna ◽  
Reactive Oxygen ◽  
Multidrug Resistant ◽  
Oxygen Species ◽  
Mammalian Cell Lines ◽  
Parasite Plasmodium Falciparum

ABSTRACT The emergence of multidrug-resistant parasites is a major concern for malaria control, and development of novel drugs is a high priority. Curcumin, a natural polyphenolic compound, possesses diverse pharmacological properties. Among its antiprotozoan activities, curcumin was potent against both chloroquine-sensitive and -resistant Plasmodium falciparum strains. Consistent with findings in mammalian cell lines, curcumin's prooxidant activity promoted the production in P. falciparum of reactive oxygen species (ROS), whose cytotoxic effect could be antagonized by coincubation with antioxidants and ROS scavengers. Curcumin treatment also resulted in damage of both mitochondrial and nuclear DNA, probably due to the elevation of intracellular ROS. Furthermore, we have demonstrated that curcumin inhibited the histone acetyltransferase (HAT) activity of the recombinant P. falciparum general control nonderepressed 5 (PfGCN5) in vitro and reduced nuclear HAT activity of the parasite in culture. Curcumin-induced hypoacetylation of histone H3 at K9 and K14, but not H4 at K5, K8, K12, and K16, suggested that curcumin caused specific inhibition of the PfGCN5 HAT. Taken together, these results indicated that at least the generation of ROS and down-regulation of PfGCN5 HAT activity accounted for curcumin's cytotoxicity for malaria parasites.

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