scholarly journals Specific replication origins promote DNA amplification in fission yeast

2010 ◽  
Vol 123 (18) ◽  
pp. 3047-3051 ◽  
Author(s):  
L. Kiang ◽  
C. Heichinger ◽  
S. Watt ◽  
J. Bahler ◽  
P. Nurse
Keyword(s):  
Fission Yeast ◽  
Dna Amplification ◽  
Replication Origins

scholarly journals Nucleosomal organization of replication origins and meiotic recombination hotspots in fission yeast

2011 ◽  
Vol 31 (1) ◽  
pp. 124-137 ◽  
Author(s):  
Elisa de Castro ◽  
Ignacio Soriano ◽  
Laura Marín ◽  
Rebeca Serrano ◽  
Luis Quintales ◽  
...  
Keyword(s):  
Fission Yeast ◽  
Meiotic Recombination ◽  
Replication Origins ◽  
Recombination Hotspots ◽  
Meiotic Recombination Hotspots

scholarly journals DNA Replication Origins Fire Stochastically in Fission Yeast

2006 ◽  
Vol 17 (1) ◽  
pp. 308-316 ◽  
Author(s):  
Prasanta K. Patel ◽  
Benoit Arcangioli ◽  
Stephen P. Baker ◽  
Aaron Bensimon ◽  
Nicholas Rhind
Keyword(s):  
Dna Replication ◽  
Fission Yeast ◽  
Replication Initiation ◽  
Epigenetic Mechanism ◽  
Replication Origins ◽  
Origin Firing ◽  
Cell Cycles ◽  
Initiation Of Replication ◽  
Dna Combing ◽  
Dna Replication Origins

DNA replication initiates at discrete origins along eukaryotic chromosomes. However, in most organisms, origin firing is not efficient; a specific origin will fire in some but not all cell cycles. This observation raises the question of how individual origins are selected to fire and whether origin firing is globally coordinated to ensure an even distribution of replication initiation across the genome. We have addressed these questions by determining the location of firing origins on individual fission yeast DNA molecules using DNA combing. We show that the firing of replication origins is stochastic, leading to a random distribution of replication initiation. Furthermore, origin firing is independent between cell cycles; there is no epigenetic mechanism causing an origin that fires in one cell cycle to preferentially fire in the next. Thus, the fission yeast strategy for the initiation of replication is different from models of eukaryotic replication that propose coordinated origin firing.

scholarly journals Genome-wide localization of pre-RC sites and identification of replication origins in fission yeast

2007 ◽  
Vol 26 (5) ◽  
pp. 1327-1339 ◽  
Author(s):  
Makoto Hayashi ◽  
Yuki Katou ◽  
Takehiko Itoh ◽  
Mitsutoshi Tazumi ◽  
Yoshiki Yamada ◽  
...  
Keyword(s):  
Fission Yeast ◽  
Replication Origins ◽  
Genome Wide

scholarly journals Association of Fission Yeast Orp1 and Mcm6 Proteins with Chromosomal Replication Origins

1999 ◽  
Vol 19 (10) ◽  
pp. 7228-7236 ◽  
Author(s):  
Yuya Ogawa ◽  
Tatsuro Takahashi ◽  
Hisao Masukata
Keyword(s):  
Cell Cycle ◽  
Fission Yeast ◽  
Chromatin Immunoprecipitation ◽  
Origin Recognition Complex ◽  
Replication Origins ◽  
Minichromosome Maintenance ◽  
Chromatin Immunoprecipitation Assay ◽  
Chromosomal Replication ◽  
Initiation Of Replication ◽  
Mcm Proteins

ABSTRACT We have previously shown that replication of fission yeast chromosomes is initiated in distinct regions. Analyses of autonomous replicating sequences have suggested that regions required for replication are very different from those in budding yeast. Here, we present evidence that fission yeast replication origins are specifically associated with proteins that participate in initiation of replication. Most Orp1p, a putative subunit of the fission yeast origin recognition complex (ORC), was found to be associated with chromatin-enriched insoluble components throughout the cell cycle. In contrast, the minichromosome maintenance (Mcm) proteins, SpMcm2p and SpMcm6p, encoded by thenda1 +/cdc19+ andmis5+ genes, respectively, were associated with chromatin DNA only during the G1 and S phases. Immunostaining of spread nuclei showed SpMcm6p to be localized at discrete foci on chromatin during the G1 and S phases. A chromatin immunoprecipitation assay demonstrated that Orp1p was preferentially localized at the ars2004 andars3002 origins of the chromosome throughout the cell cycle, while SpMcm6p was associated with these origins only in the G1 and S phases. Both Orp1p and SpMcm6p were associated with a 1-kb region that contains elements required for autonomous replication of ars2004. The results suggest that the fission yeast ORC specifically interacts with chromosomal replication origins and that Mcm proteins are loaded onto the origins to play a role in initiation of replication.

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