scholarly journals LC3-mediated fibronectin mRNA translation induces fibrosarcoma growth by increasing connective tissue growth factor

2009 ◽  
Vol 122 (9) ◽  
pp. 1441-1451 ◽  
Author(s):  
L. Ying ◽  
A. Lau ◽  
C. M. Alvira ◽  
R. West ◽  
G. M. Cann ◽  
...  
Keyword(s):  
Growth Factor ◽  
Connective Tissue ◽  
Connective Tissue Growth Factor ◽  
Mrna Translation ◽  
Tissue Growth ◽  
Fibronectin Mrna

Role of connective tissue growth factor in fibronectin expression and tubulointerstitial fibrosis

2002 ◽  
Vol 282 (5) ◽  
pp. F933-F942 ◽  
Author(s):  
Hideki Yokoi ◽  
Masashi Mukoyama ◽  
Akira Sugawara ◽  
Kiyoshi Mori ◽  
Tetsuya Nagae ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Connective Tissue ◽  
Connective Tissue Growth Factor ◽  
Tissue Growth ◽  
Tubulointerstitial Fibrosis ◽  
Type I ◽  
Fibronectin Expression ◽  
Tgf Β1 ◽  
Fibronectin Mrna

Connective tissue growth factor (CTGF) is one of the candidate factors mediating downstream events of transforming growth factor-β (TGF-β), but its role in fibrogenic properties of TGF-β and in tubulointerstitial fibrosis has not yet been clarified. Using unilateral ureteral obstruction (UUO) in rats, we analyzed gene expression of TGF-β1, CTGF, and fibronectin. We further investigated the effect of blockade of endogenous CTGF on TGF-β-induced fibronectin expression in cultured rat renal fibroblasts by antisense oligodeoxynucleotide (ODN) treatment. After UUO, CTGF mRNA expression in the obstructed kidney was significantly upregulated subsequent to TGF-β1, followed by marked induction of fibronectin mRNA. By in situ hybridization, CTGF mRNA was detected mainly in the interstitial fibrotic areas and tubular epithelial cells as well as in parietal glomerular epithelial cells in the obstructed kidney. The interstitial cells expressing CTGF mRNA were also positive for α-smooth muscle actin. CTGF antisense ODN transfected into cultured renal fibroblasts significantly attenuated TGF-β-stimulated upregulation of fibronectin mRNA and protein compared with control ODN transfection, together with inhibited synthesis of type I collagen. With the use of a reporter assay, rat fibronectin promoter activity was increased by 2.5-fold with stimulation by TGF-β1, and this increase was abolished with antisense CTGF treatment. Thus CTGF plays a crucial role in fibronectin synthesis induced by TGF-β, suggesting that CTGF blockade could be a possible therapeutic target against tubulointerstitial fibrosis.

Identification of connective tissue growth factor as a hepatic negative acute phase protein

Hepatology ◽  
2009 ◽  
pp. NA-NA
Author(s):  
Ieva Peredniene ◽  
Eddy van de Leur ◽  
Birgit Lahme ◽  
Monika Siluschek ◽  
Axel M. Gressner ◽  
...  
Keyword(s):  
Growth Factor ◽  
Connective Tissue ◽  
Connective Tissue Growth Factor ◽  
Acute Phase ◽  
Acute Phase Protein ◽  
Tissue Growth ◽  
Phase Protein
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