scholarly journals Unconventional myosin VIIa and vezatin, two proteins crucial for Listeria entry into epithelial cells

2004 ◽  
Vol 117 (10) ◽  
pp. 2121-2130 ◽  
Author(s):  
S. Sousa
Keyword(s):  
Epithelial Cells ◽  
Unconventional Myosin ◽  
Myosin Viia

scholarly journals Uncoated Endocytic Vesicles Require the Unconventional Myosin, Myo6, for Rapid Transport through Actin Barriers

2004 ◽  
Vol 15 (5) ◽  
pp. 2253-2263 ◽  
Author(s):  
Laura Aschenbrenner ◽  
Samia N. Naccache ◽  
Tama Hasson
Keyword(s):  
Epithelial Cells ◽  
Motor Activity ◽  
Time Lapse ◽  
Early Endosome ◽  
Physical Barrier ◽  
Unconventional Myosin ◽  
Time Lapse Microscopy ◽  
Rapid Transport ◽  
Endocytic Vesicles

After clathrin-mediated endocytosis, clathrin removal yields an uncoated vesicle population primed for fusion with the early endosome. Here we present the first characterization of uncoated vesicles and show that myo6, an unconventional myosin, functions to move these vesicles out of actin-rich regions found in epithelial cells. Time-lapse microscopy revealed that myo6-associated uncoated vesicles were motile and exhibited fusion and stretching events before endosome delivery, processes that were dependent on myo6 motor activity. In the absence of myo6 motor activity, uncoated vesicles remained trapped in the actin mesh, where they exhibited Brownian-like motion. Exit from the actin mesh occurred by a slow diffusion-based mechanism, delaying transferrin trafficking to the early endosome. Expression of a myo6 mutant that bound tightly to F-actin produced immobilized vesicles and blocked trafficking. Depolymerization of the actin cytoskeleton rescued this block and specifically accelerated transferrin delivery to the early endosome without affecting earlier steps in endocytosis. Therefore actin is a physical barrier impeding uncoated vesicle trafficking, and myo6 is recruited to move the vesicles through this barrier for fusion with the early endosome.

scholarly journals Unconventional Myosin VIIA Is a Novel A-kinase-anchoring Protein

2000 ◽  
Vol 275 (38) ◽  
pp. 29654-29659 ◽  
Author(s):  
Polonca Küssel-Andermann ◽  
Aziz El-Amraoui ◽  
Saaid Safieddine ◽  
Jean-Pierre Hardelin ◽  
Sylvie Nouaille ◽  
...  
Keyword(s):  
Unconventional Myosin ◽  
Anchoring Protein ◽  
Myosin Viia

scholarly journals The unconventional myosin-VIIa associates with lysosomes

2005 ◽  
Vol 62 (1) ◽  
pp. 13-26 ◽  
Author(s):  
Lily E. Soni ◽  
Carmen M. Warren ◽  
Cecilia Bucci ◽  
Dana J. Orten ◽  
Tama Hasson
Keyword(s):  
Unconventional Myosin ◽  
Myosin Viia

scholarly journals Unconventional Myosins in Inner-Ear Sensory Epithelia

1997 ◽  
Vol 137 (6) ◽  
pp. 1287-1307 ◽  
Author(s):  
Tama Hasson ◽  
Peter G. Gillespie ◽  
Jesus A. Garcia ◽  
Richard B. MacDonald ◽  
Yi-dong Zhao ◽  
...  
Keyword(s):  
Inner Ear ◽  
Hair Cells ◽  
Actin Filaments ◽  
Structural Integrity ◽  
Myosin Vi ◽  
Cortical Actin ◽  
Unconventional Myosin ◽  
Myosin Isozymes ◽  
Cuticular Plate ◽  
Myosin Viia

To understand how cells differentially use the dozens of myosin isozymes present in each genome, we examined the distribution of four unconventional myosin isozymes in the inner ear, a tissue that is particularly reliant on actin-rich structures and unconventional myosin isozymes. Of the four isozymes, each from a different class, three are expressed in the hair cells of amphibia and mammals. In stereocilia, constructed of cross-linked F-actin filaments, myosin-Iβ is found mostly near stereociliary tips, myosin-VI is largely absent, and myosin-VIIa colocalizes with crosslinks that connect adjacent stereocilia. In the cuticular plate, a meshwork of actin filaments, myosin-Iβ is excluded, myosin-VI is concentrated, and modest amounts of myosin-VIIa are present. These three myosin isozymes are excluded from other actin-rich domains, including the circumferential actin belt and the cortical actin network. A member of a fourth class, myosin-V, is not expressed in hair cells but is present at high levels in afferent nerve cells that innervate hair cells. Substantial amounts of myosins-Iβ, -VI, and -VIIa are located in a pericuticular necklace that is largely free of F-actin, squeezed between (but not associated with) actin of the cuticular plate and the circumferential belt. Our localization results suggest specific functions for three hair-cell myosin isozymes. As suggested previously, myosin-Iβ probably plays a role in adaptation; concentration of myosin-VI in cuticular plates and association with stereociliary rootlets suggest that this isozyme participates in rigidly anchoring stereocilia; and finally, colocalization with cross-links between adjacent stereocilia indicates that myosin-VIIa is required for the structural integrity of hair bundles.

scholarly journals MARCKS and Related Chaperones Bind to Unconventional Myosin V Isoforms in Airway Epithelial Cells

2010 ◽  
Vol 43 (2) ◽  
pp. 131-136 ◽  
Author(s):  
Ko-Wei Lin ◽  
Shijing Fang ◽  
Joungjoa Park ◽  
Anne L. Crews ◽  
Kenneth B. Adler
Keyword(s):  
Epithelial Cells ◽  
Airway Epithelial Cells ◽  
Airway Epithelial ◽  
Myosin V ◽  
Unconventional Myosin

scholarly journals Unconventional myosin VIIA promotes melanoma progression

2018 ◽  
Vol 131 (4) ◽  
pp. jcs209924 ◽  
Author(s):  
Yuqing Liu ◽  
Xiaofan Wei ◽  
Lizhao Guan ◽  
Sidi Xu ◽  
Yang Yuan ◽  
...  
Keyword(s):  
Unconventional Myosin ◽  
Melanoma Progression ◽  
Myosin Viia

Electron Microscopic Study of the Epithelium of the Seminal Vesicle of Aging Rats

1974 ◽  
Vol 32 ◽  
pp. 138-139
Author(s):  
V. F. Allison ◽  
G. C. Fink ◽  
G. W. Cearley
Keyword(s):  
Cell Growth ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Seminal Vesicle ◽  
Seminal Vesicles ◽  
Epithelial Layer ◽  
Epithelial Hyperplasia ◽  
Electron Microscopic ◽  
Aging Rats ◽  
Pseudostratified Epithelium

It is well known that epithelial hyperplasia (benign hypertrophy) is common in the aging prostate of dogs and man. In contrast, little evidence is available for abnormal epithelial cell growth in seminal vesicles of aging animals. Recently, enlarged seminal vesicles were reported in senescent mice, however, that enlargement resulted from increased storage of secretion in the lumen and occurred concomitant to epithelial hypoplasia in that species.The present study is concerned with electron microscopic observations of changes occurring in the pseudostratified epithelium of the seminal vescles of aging rats. Special attention is given to certain non-epithelial cells which have entered the epithelial layer.

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