scholarly journals Analysis of the interactions between BP180, BP230, plectin and the integrin alpha6beta4 important for hemidesmosome assembly

2002 ◽  
Vol 116 (2) ◽  
pp. 387-399 ◽  
Author(s):  
J. Koster
Keyword(s):  
Integrin Alpha6beta4

scholarly journals Human amnion contains a novel laminin variant, laminin 7, which like laminin 6, covalently associates with laminin 5 to promote stable epithelial-stromal attachment.

1996 ◽  
Vol 132 (6) ◽  
pp. 1189-1198 ◽  
Author(s):  
M F Champliaud ◽  
G P Lunstrum ◽  
P Rousselle ◽  
T Nishiyama ◽  
D R Keene ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Tight Binding ◽  
Human Amnion ◽  
Laminin 5 ◽  
Integrin Alpha6beta4 ◽  
Epithelial Basement Membrane ◽  
Stable Association ◽  
Membrane Components

Stable attachment of external epithelia to the basement membrane and underlying stroma is mediated by transmembrane proteins such as the integrin alpha6beta4 and bullous pemphigoid antigen 2 within the hemidesmosomes along the basolateral surface of the epithelial cell and their ligands that include a specialized subfamily of laminins. The laminin 5 molecule (previously termed kalinin/nicein/epiligrin) is a member of this epithelial-specific subfamily. Laminin 5 chains are not only considerably truncated within domains III-VI, but are also extensively proteolytically processed in vitro and in vivo. As a result, the domains expected to be required for the association of laminins with other basement membrane components are lacking in the mature laminin 5 molecule. Therefore, the tight binding of laminin 5 to the basement membrane may occur by a unique mechanism. To examine laminin 5 in tissue, we chose human amnion as the source, because of its availability and the similarity of the amniotic epithelial basement membrane with that of skin. We isolated the laminin 5 contained within the basement membrane of human amnion. In addition to monomeric laminin 5, we find that much of the laminin 5 isolated is covalently adducted with laminin 6 (alpha3beta1gamma1) and a novel laminin isotype we have termed laminin 7 (alpha3beta2gamma1). We propose that the association between laminin 5 and laminins 6 and 7 is a mechanism used in amnion to allow stable association of laminin 5 with the basement membrane. The beta2 chain is seen at the human amniotic epithelial-stromal interface and at the dermal-epidermal junction of fetal and adult bovine skin by immunofluorescence, but is not present, or only weakly present, in neonatal human skin.

scholarly journals FC-26 Junctional epidermolysis bullosa in a Charolais calf with deficient expression of integrin alpha6beta4

2004 ◽  
Vol 15 (s1) ◽  
pp. 28-28 ◽  
Author(s):  
E. Guaguere ◽  
K. Berg ◽  
F. Degorce-Rubiales ◽  
A. Spadafora ◽  
G. Meneguzzi
Keyword(s):  
Epidermolysis Bullosa ◽  
Junctional Epidermolysis Bullosa ◽  
Integrin Alpha6beta4
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