scholarly journals Outer mitochondrial membrane permeabilization during apoptosis triggers caspase-independent mitochondrial and caspase-dependent plasma membrane potential depolarization: a single-cell analysis

2002 ◽  
Vol 116 (3) ◽  
pp. 525-536 ◽  
Author(s):  
H. Dussmann
Keyword(s):  
Plasma Membrane ◽  
Membrane Potential ◽  
Single Cell ◽  
Mitochondrial Membrane ◽  
Single Cell Analysis ◽  
Membrane Permeabilization ◽  
Outer Mitochondrial Membrane ◽  
Cell Analysis ◽  
Plasma Membrane Potential ◽  
Mitochondrial Membrane Permeabilization

scholarly journals Dynamics of outer mitochondrial membrane permeabilization during apoptosis

2009 ◽  
Vol 16 (4) ◽  
pp. 613-623 ◽  
Author(s):  
M Rehm ◽  
H J Huber ◽  
C T Hellwig ◽  
S Anguissola ◽  
H Dussmann ◽  
...  
Keyword(s):  
Mitochondrial Membrane ◽  
Membrane Permeabilization ◽  
Outer Mitochondrial Membrane ◽  
Mitochondrial Membrane Permeabilization

scholarly journals Single-cell analysis of pyroptosis dynamics reveals conserved GSDMD-mediated subcellular events that precede plasma membrane rupture

2018 ◽  
Vol 26 (1) ◽  
pp. 146-161 ◽  
Author(s):  
Nathalia M. de Vasconcelos ◽  
Nina Van Opdenbosch ◽  
Hanne Van Gorp ◽  
Eef Parthoens ◽  
Mohamed Lamkanfi
Keyword(s):  
Plasma Membrane ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Membrane Rupture

scholarly journals Energy transduction in intact synaptosomes. Influence of plasma-membrane depolarization on the respiration and membrane potential of internal mitochondria determined in situ

1980 ◽  
Vol 186 (1) ◽  
pp. 21-33 ◽  
Author(s):  
I D Scott ◽  
D G Nicholls
Keyword(s):  
Plasma Membrane ◽  
Membrane Potential ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Significant Proportion ◽  
Membrane Potentials ◽  
Simultaneous Estimation ◽  
Anaerobic Glycolysis ◽  
Mitochondrial Potential ◽  
Plasma Membrane Potential

A method is described, based on the differential accumulation of Rb+ and methyltriphenylphosphonium, for the simultaneous estimation of the membrane potentials across the plasma membrane of isolated nerve endings (synaptosomes), and across the inner membrane of mitochondria within the synaptosomal cytoplasm. These determinations, together with measurements of respiratory rates, and ATP and phosphocreatine concentrations, are used to define the bioenergetic behaviour of isolated synaptosomes under a variety of conditions. Under control conditions, in the presence of glucose, the plasma and mitochondrial membrane potentials are respectively 45 and 148mV. Addition of a proton translocator induces a 5-fold increase in respiration, and abolishes the mitochondrial membrane potential. The addition of rotenone to inhibit respiration does not affect the plasma membrane potential, and only lowers the mitochondrial membrane potential to 128mV. Evidence is presented that ATP synthesis by anaerobic glycolysis is sufficient under these conditions to maintain ATP-dependent processes, including the reversal of the mitochondrial ATP synthetase. Addition of oligomycin under non-respiring conditions leads to a complete collapse of the mitochondrial potential. Even under control conditions the plasma membrane (Na+ + K+)-dependent ATPase is responsible for a significant proportion of the synaptosomal ATP turnover. Veratridine greatly increases respiration, and depolarizes the plasma membrane, but only slightly lowers the mitochondrial membrane potential. High K+ and ouabain also lower the plasma membrane potential without decreasing the mitochondrial membrane potential. In non-respiring synaptosomes, anaerobic glycolysis is incapable of maintaining cytosolic ATP during the increased turnover induced by veratridine, and the mitochondrial membrane potential collapses. It is concluded that the internal mitochondria must be considered in any study of synaptosomal transport.

Author response for "Immunocyte single cell analysis of vaccine‐induced narcolepsy"

2020 ◽  
Author(s):  
Alexander Lind ◽  
Falastin Salami ◽  
Anne‐Marie Landtblom ◽  
Lars Palm ◽  
Åke Lernmark ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Author Response ◽  
Cell Analysis
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