scholarly journals Human embryoid bodies as a 3D tissue model of the extracellular matrix and α-dystroglycanopathies

2020 ◽  
Vol 13 (6) ◽  
pp. dmm042986
Author(s):  
Alec R. Nickolls ◽  
Michelle M. Lee ◽  
Kristen Zukosky ◽  
Barbara S. Mallon ◽  
Carsten G. Bönnemann
Keyword(s):  
Extracellular Matrix ◽  
Embryoid Bodies ◽  
Tissue Model

scholarly journals Patterning of Growth Factor Gradients in Embryoid Bodies via Extracellular Matrix-Assisted Glycocalyx Engineering

2020 ◽  
Author(s):  
Matthew R. Naticchia ◽  
Logan K. Laubach ◽  
honigfort Daniel J. ◽  
purcell Sean C. ◽  
Kamil Godula
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Growth Factor ◽  
Cell Surface ◽  
Surface Engineering ◽  
Embryoid Bodies ◽  
Cell Surface Engineering

Cell surface engineering with synthetic glycomimetic co-receptors for FGF2 was used to establish gradients of stem cells with enhanced FGF2 affinity in embryoid bodies (EBs). Gradient shape was controlled by pre-assembly of glycomimetics into nanoscale vesicles with tunable dimensions and EB penetrance. <br>

scholarly journals The versican-hyaluronan complex provides an essential extracellular matrix niche for Flk1+ hematoendothelial progenitors

2019 ◽  
Author(s):  
Sumeda Nandadasa ◽  
Anna O’Donnell ◽  
Ayako Murao ◽  
Yu Yamaguchi ◽  
Ronald J. Midura ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Embryonic Stem ◽  
Yolk Sac ◽  
Embryoid Bodies ◽  
Mouse Embryos ◽  
Indian Hedgehog ◽  
Vascular Endothelial ◽  
Angiogenic Response ◽  
Cell Lineages ◽  
Primitive Hematopoiesis

AbstractLittle is known about extracellular matrix (ECM) contributions to formation of the earliest cell lineages in the embryo. Here, we show that the proteoglycan versican and glycosaminoglycan hyaluronan are associated with emerging Flk1+ hematoendothelial progenitors at gastrulation. The mouse versican mutant Vcanhdf lacks yolk sac vasculature, with attenuated yolk sac hematopoiesis. CRISPR/Cas9-mediated Vcan inactivation in mouse embryonic stem cells reduced vascular endothelial and hematopoietic differentiation in embryoid bodies, which generated fewer blood colonies, and had an impaired angiogenic response to VEGF165. HA was severely depleted in Vcanhdf embryos, with corresponding increase in the HA-depolymerase TMEM2. Conversely, HA-deficient mouse embryos also had vasculogenic suppression but with increased versican proteolysis. VEGF165 and Indian hedgehog, crucial vasculogenic factors, utilized the versican-HA matrix, specifically versican chondroitin sulfate chains, for binding. Versican-HA ECM is an obligate requirement for vasculogenesis and primitive hematopoiesis, acts as an vasculogenic factor-enriching microniche for Flk1+ progenitors from their origin at gastrulation.

Microfluidic vascularized bone tissue model with hydroxyapatite-incorporated extracellular matrix

Lab on a Chip ◽  
2015 ◽  
Vol 15 (20) ◽  
pp. 3984-3988 ◽  
Author(s):  
Norhana Jusoh ◽  
Soojung Oh ◽  
Sudong Kim ◽  
Jangho Kim ◽  
Noo Li Jeon
Keyword(s):  
Extracellular Matrix ◽  
Bone Tissue ◽  
Microfluidic Device ◽  
Three Dimensional ◽  
Microvascular Networks ◽  
Tissue Model ◽  
Vascularized Bone

We propose three-dimensional microvascular networks in a hydroxyapatite-incorporated extracellular matrix for designing and manipulating a vascularized bone tissue model in a microfluidic device.

scholarly journals Extracellular Matrix and Integrin Expression Profiles in Fuchs Endothelial Corneal Dystrophy Cells and Tissue Model

2018 ◽  
Vol 24 (7-8) ◽  
pp. 607-615 ◽  
Author(s):  
Benjamin Goyer ◽  
Mathieu Thériault ◽  
Sébastien P. Gendron ◽  
Isabelle Brunette ◽  
Patrick J. Rochette ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Expression Profiles ◽  
Corneal Dystrophy ◽  
Integrin Expression ◽  
Tissue Model

scholarly journals A three-dimensional in vitro dynamic micro-tissue model of cardiac scar formation

2018 ◽  
Vol 10 (3) ◽  
pp. 174-183 ◽  
Author(s):  
Paola Occhetta ◽  
Giuseppe Isu ◽  
Marta Lemme ◽  
Chiara Conficconi ◽  
Philipp Oertle ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Three Dimensional ◽  
Scar Formation ◽  
Fibroblast Proliferation ◽  
Tissue Model ◽  
Matrix Deposition ◽  
Extracellular Matrix Deposition ◽  
Mechanical Stretching

Our 3D-scar-on-a-chip model resembles fibroblast proliferation and activation, extracellular matrix deposition and stiffening upon application of only cyclic mechanical stretching.

Expression of extracellular matrix-degrading metalloproteinases and metalloproteinase inhibitors is developmentally regulated during endoderm differentiation of embryonal carcinoma cells

Development ◽  
1990 ◽  
Vol 110 (1) ◽  
pp. 211-220 ◽  
Author(s):  
R.R. Adler ◽  
C.A. Brenner ◽  
Z. Werb
Keyword(s):  
Extracellular Matrix ◽  
Embryonal Carcinoma ◽  
Embryoid Bodies ◽  
Carcinoma Cells ◽  
Tissue Inhibitor Of Metalloproteinases ◽  
Embryonal Carcinoma Cells ◽  
Developmentally Regulated ◽  
F9 Cells ◽  
Metalloproteinase Inhibitors ◽  
Parietal Endoderm

The differentiation of F9 and PSA-1 embryonal carcinoma cells to embryoid bodies composed of a mixture of parietal and visceral endoderm was accompanied by changes in their secretion of metalloproteinases. Differentiation was induced by retinoic acid and dibutyryl cyclic AMP (for F9 cells) or by removing cells from a substrate of feeder cells to alter cell-cell interaction and adhesion (for PSA-1 cells). The embryoid bodies attached to gelatin-coated dishes, and the parietal endoderm cells spread out over the matrix. The differentiated cells secreted specific gelatin- and casein-degrading proteinases, including enzymes that comigrated with proenzyme forms of collagenase and stromelysin. Total proteinase activity as well as specific collagenase activity increased with the time of differentiation. All of the gelatin- and casein-degrading proteinases detectable by substrate gel zymography were inhibited by inhibitors of metalloproteinases but not by inhibitors of serine or cysteine proteinases, indicating that they were metalloproteinases. Both cell lines showed increased collagenolytic activity, which was activated by treatment with plasmin. In addition, both cell lines showed increased secretion of specific metalloproteinase inhibitors, including tissue inhibitor of metalloproteinases, with differentiation. Analysis of mRNA from undifferentiated and differentiated F9 cells by RNA blot analysis or reverse transcription coupled with the polymerase chain reaction showed that increased expression of genes for collagenase, stromelysin and tissue inhibitor of metalloproteinases is associated with differentiation of these cells. These results suggest that the expression of extracellular matrix-degrading metalloproteinases and their inhibitors is developmentally regulated during the differentiation and spreading of the parietal endoderm.

Preparation of Extracellular Matrix Paper and Construction of Multi‐Layered 3D Tissue Model

2020 ◽  
Vol 88 (1) ◽  
Author(s):  
Hirotaka Nakatsuji ◽  
Shiro Kitano ◽  
Shinji Irie ◽  
Michiya Matsusaki
Keyword(s):  
Extracellular Matrix ◽  
Tissue Model
Sign in / Sign up

Export Citation Format

Share Document