scholarly journals Next-generation RNA sequencing of FFPE subsections reveals highly conserved stromal reprogramming between canine and human mammary carcinoma

2019 ◽  
Vol 12 (8) ◽  
pp. dmm040444 ◽  
Author(s):  
Parisa Amini ◽  
Sina Nassiri ◽  
Julia Ettlin ◽  
Alexandra Malbon ◽  
Enni Markkanen
Keyword(s):  
Rna Sequencing ◽  
Mammary Carcinoma ◽  
Next Generation ◽  
Human Mammary Carcinoma

The Ultrastructure of Metastatic Human Mammary Carcinoma After Prolonged Estrogen Therapy

1967 ◽  
Vol 25 ◽  
pp. 180-181
Author(s):  
John H.L. Watson ◽  
John L. Swedo ◽  
R.W. Talley
Keyword(s):  
Mammary Carcinoma ◽  
Osmium Tetroxide ◽  
Estrogen Therapy ◽  
Ultrastructural Level ◽  
Uranyl Acetate ◽  
Human Mammary Carcinoma ◽  
Surgical Biopsy ◽  
Lead Hydroxide ◽  
Preliminary Study ◽  
Hormonal Therapies

A preliminary study of human mammary carcinoma on the ultrastructural level is reported for a metastatic, subcutaneous nodule, obtained as a surgical biopsy. The patient's tumor had responded favorably to a series of hormonal therapies, including androgens, estrogens, progestins, and corticoids for recurring nodules over eight years. The pertinent nodule was removed from the region of the gluteal maximus, two weeks following stilbestrol therapy. It was about 1.5 cms in diameter, and was located within the dermis. Pieces from it were fixed immediately in cold fixatives: phosphate buffered osmium tetroxide, glutaraldehyde, and paraformaldehyde. Embedment in each case was in Vestopal W. Contrasting was done with combinations of uranyl acetate and lead hydroxide.

scholarly journals Suitability of Bronchoscopic Biopsy Tissue Samples for Next-Generation Sequencing

Diagnostics ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 391
Author(s):  
Shuji Murakami ◽  
Tomoyuki Yokose ◽  
Daiji Nemoto ◽  
Masaki Suzuki ◽  
Ryou Usui ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Success Rate ◽  
Rna Sequencing ◽  
Surface Area ◽  
Endobronchial Ultrasound ◽  
Next Generation ◽  
Endobronchial Ultrasonography ◽  
Tissue Surface ◽  
The Impact ◽  
Generation Sequencing

A sufficiently large tissue sample is required to perform next-generation sequencing (NGS) with a high success rate, but the majority of patients with advanced non-small-cell lung cancer (NSCLC) are diagnosed with small biopsy specimens. Biopsy samples were collected from 184 patients with bronchoscopically diagnosed NSCLC. The tissue surface area, tumor cell count, and tumor content rate of each biopsy sample were evaluated. The impact of the cut-off criteria for the tissue surface area (≥1 mm2) and tumor content rate (≥30%) on the success rate of the Oncomine Dx Target Test (ODxTT) was evaluated. The mean tissue surface area of the transbronchial biopsies was 1.23 ± 0.85 mm2 when small endobronchial ultrasonography with a guide sheath (EBUS-GS) was used, 2.16 ± 1.49 mm2 with large EBUS-GS, and 1.81 ± 0.75 mm2 with endobronchial biopsy (EBB). The proportion of samples with a tissue surface area of ≥1 mm2 was 48.8% for small EBUS-GS, 79.2% for large EBUS-GS, and 78.6% for EBB. Sixty-nine patients underwent ODxTT. The success rate of DNA sequencing was 84.1% and that of RNA sequencing was 92.7% over all patients. The success rate of DNA (RNA) sequencing was 57.1% (71.4%) for small EBUS-GS (n = 14), 93.4% (96.9%) for large EBUS-GS (n = 32), 62.5% (100%) for EBB (n = 8), and 100% (100%) for endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) (n = 15). Regardless of the device used, a tissue surface area of ≥ 1 mm2 is adequate for samples to be tested with NGS.

scholarly journals Utility of next-generation RNA-sequencing in identifying chimeric transcription involving human endogenous retroviruses

Apmis ◽  
2016 ◽  
Vol 124 (1-2) ◽  
pp. 127-139 ◽  
Author(s):  
Martin Sokol ◽  
Karen Margrethe Jessen ◽  
Finn Skou Pedersen
Keyword(s):  
Rna Sequencing ◽  
Endogenous Retroviruses ◽  
Next Generation ◽  
Human Endogenous Retroviruses

scholarly journals Gestational Psittacosis Resulting in Neonatal Death Identified by Next-Generation RNA Sequencing of Postmortem, Formalin-Fixed Lung Tissue

2018 ◽  
Vol 5 (8) ◽  
Author(s):  
Litty Paul ◽  
Jessica Comstock ◽  
Kornelia Edes ◽  
Robert Schlaberg
Keyword(s):  
Rna Sequencing ◽  
Infant Death ◽  
Neonatal Death ◽  
Pathogen Detection ◽  
Severe Disease ◽  
Adverse Outcomes ◽  
Premature Delivery ◽  
Next Generation ◽  
Death Studies ◽  
Formalin Fixed

Abstract Psittacosis is a rare zoonosis that can cause severe disease and adverse outcomes during pregnancy. We identified a previously elusive case of psittacosis causing premature delivery and infant death by next-generation RNA sequencing of postmortem tissues. Hypothesis-free pathogen detection in postmortem specimens can increase the yield of epidemiologic and cause-of-death studies.

scholarly journals MicroRNA Expression Profile in Penile Cancer Revealed by Next-Generation Small RNA Sequencing

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0131336 ◽  
Author(s):  
Li Zhang ◽  
Pengfei Wei ◽  
Xudong Shen ◽  
Yuanwei Zhang ◽  
Bo Xu ◽  
...  
Keyword(s):  
Rna Sequencing ◽  
Expression Profile ◽  
Small Rna ◽  
Penile Cancer ◽  
Microrna Expression ◽  
Small Rna Sequencing ◽  
Next Generation ◽  
Microrna Expression Profile

scholarly journals Using next-generation RNA sequencing to identify imprinted genes

Heredity ◽  
2014 ◽  
Vol 113 (2) ◽  
pp. 156-166 ◽  
Author(s):  
X Wang ◽  
A G Clark
Keyword(s):  
Rna Sequencing ◽  
Imprinted Genes ◽  
Next Generation
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