scholarly journals Immortalized Parkinson's disease lymphocytes have enhanced mitochondrial respiratory activity

2016 ◽  
Vol 9 (11) ◽  
pp. 1295-1305 ◽  
Author(s):  
Sarah J. Annesley ◽  
Sui T. Lay ◽  
Shawn W. De Piazza ◽  
Oana Sanislav ◽  
Eleanor Hammersley ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Respiratory Activity ◽  
Mitochondrial Respiratory Activity ◽  
Mitochondrial Respiratory

Mitochondrial respiratory chain disorganization in Parkinson's disease-relevant PINK1 and DJ1 mutants

2017 ◽  
Vol 109 ◽  
pp. 101-105 ◽  
Author(s):  
Irene Lopez-Fabuel ◽  
Lucia Martin-Martin ◽  
Monica Resch-Beusher ◽  
Garikoitz Azkona ◽  
Rosario Sanchez-Pernaute ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Respiratory Chain ◽  
Mitochondrial Respiratory Chain ◽  
Mitochondrial Respiratory

Heme Biosynthetic Pathway is Functionally Linked to Adipogenesis via Mitochondrial Respiratory Activity

Obesity ◽  
2017 ◽  
Vol 25 (10) ◽  
pp. 1723-1733 ◽  
Author(s):  
José María Moreno-Navarrete ◽  
Amaia Rodríguez ◽  
Francisco Ortega ◽  
Sara Becerril ◽  
Jordi Girones ◽  
...  
Keyword(s):  
Biosynthetic Pathway ◽  
Respiratory Activity ◽  
Mitochondrial Respiratory Activity ◽  
Mitochondrial Respiratory

Effect of aging and dopaminomimetic therapy on mitochondrial respiratory function in Parkinson's disease

1992 ◽  
Vol 7 (3) ◽  
pp. 228-231 ◽  
Author(s):  
Daniele Bravi ◽  
Jeffrey J. Anderson ◽  
Fiorenzo Dagani ◽  
Thomas L. Davis ◽  
Rosaria Ferrari ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Respiratory Function ◽  
Mitochondrial Respiratory

Mitochondrial respiratory activity and superoxide radical generation in the liver, brain and heart after chronic ethanol intake

1994 ◽  
Vol 47 (10) ◽  
pp. 1827-1833 ◽  
Author(s):  
Catherine Ribiere ◽  
Isabelle Hininger ◽  
Chantal Saffar-Boccara ◽  
Dominique Sabourault ◽  
Roger Nordmann
Keyword(s):  
Superoxide Radical ◽  
Respiratory Activity ◽  
Ethanol Intake ◽  
Chronic Ethanol ◽  
Radical Generation ◽  
Mitochondrial Respiratory Activity ◽  
Mitochondrial Respiratory

scholarly journals Clock-genes and mitochondrial respiratory activity: Evidence of a reciprocal interplay

2016 ◽  
Vol 1857 (8) ◽  
pp. 1344-1351 ◽  
Author(s):  
Rosella Scrima ◽  
Olga Cela ◽  
Giuseppe Merla ◽  
Bartolomeo Augello ◽  
Rosa Rubino ◽  
...  
Keyword(s):  
Clock Genes ◽  
Respiratory Activity ◽  
Mitochondrial Respiratory Activity ◽  
Mitochondrial Respiratory

Detection of Mitochondrial Respiratory Dysfunction in Circulating Lymphocytes Using Resazurin

2005 ◽  
Vol 129 (10) ◽  
pp. 1295-1298
Author(s):  
Khaled K. Abu-Amero ◽  
Thomas M. Bosley
Keyword(s):  
Normal Control ◽  
Respiratory Activity ◽  
Mitochondrial Protein ◽  
Mitochondrial Disorders ◽  
Control Group ◽  
Respiratory Dysfunction ◽  
Control Subjects ◽  
Mitochondrial Fractions ◽  
Mitochondrial Respiratory Activity ◽  
Mitochondrial Respiratory

Abstract Context.—Laboratory methods currently available for detecting mitochondrial respiratory dysfunction are labor-intensive, require large amounts of isolated mitochondrial protein, invasive (require a skeletal muscle biopsy), and usually produce conflicting results. Objective.—To develop a rapid, reliable, and noninvasive method for detecting oxidative phosphorylation activity without the need to isolate mitochondrial fractions. Design.—Lymphocytes from 6 patients with mitochondrial disorders (3 with mitochondrial myopathy and 3 with Leber hereditary optic neuropathy) and 51 normal control subjects were incubated with 6μM resazurin without and with mitochondrial inhibition by amiodarone (200μM), and the fluorescence intensity resulting from resazurin reduction was monitored spectrofluorometrically over time. Mitochondrial respiratory activity was calculated as the difference between uninhibited and inhibited measurements. Results.—Mitochondrial respiratory activity was established for 51 normal control subjects and was decreased in all 6 patients with mitochondrial syndromes. Mitochondrial respiratory activity values for patients 1 through 6 compared to the control group after 240 minutes' incubation with resazurin were 55%, 71%, 49%, 61%, 68%, and 59%, respectively (mean mitochondrial respiratory activity of patients, 13.6% or 60.5% of control mean; P < .001). Conclusion.—This resazurin-based technique proved to be a fast and reproducible method for quantifying mitochondrial activity and identifying respiratory functional defects in patients with mitochondrial disorders.

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